Isolation and characterization of an anti-microbial biflavonoid from the chloroform-soluble fraction of methanolic root extract of Ochna schweinfurthiana (Ochnaceae)

Abdullahi, M. I.; Musa, Abdullahi; Haruna, Abdurrashid; Pateh, U. U.; Sule, Ibrahim M; Abdulmalik, I. A.; Abdullahi, M.; Abimiku, A. G.; Iliya, Ibrahim Abdullahi

doi:10.5897/ajpp2013.3520

Cited by 17 publications

(16 citation statements)

References 21 publications

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“…Plant extracts were prepared according to the method described by Ndjonka et al [ 17 ] and Abdullahi et al [ 18 ]. Briefly, 50 g of powdered plant organs were extracted in 500 mL of ethanol-distilled water (70:30) and MeOH-CH 2 Cl 2 (50:50 v /v) for 48 h at room temperature, centrifuged (3500×g, 10 min) and filtered over filter papers No.…”

Section: Methodsmentioning

confidence: 99%

“…For fractionation, dried powder of leaves (1.5 kg) and root bark (2 kg) were macerated with 4 L of MeOH-CH 2 Cl 2 (50:50 v /v) for 48 h then filtered with wattman paper No. 1 [ 18 ]. The organic solvents were concentrated under reduced pressure at 40 °C, using rotary evaporator (Buchi Rotavapor R-210, Germany) to yield crude extracts of leaves (5.24%) and root bark (3.94%) [ 19 ].…”

Section: Methodsmentioning

confidence: 99%

“…The organic solvents were concentrated under reduced pressure at 40 °C, using rotary evaporator (Buchi Rotavapor R-210, Germany) to yield crude extracts of leaves (5.24%) and root bark (3.94%) [ 19 ]. Each crude extract (78.61 g of leaves and 78.87 g of root barks’) was re-suspended in MeOH-CH 2 Cl 2 then partitioned with hexane (FH) (1:0 v /v), hexane: acetate (FHAE) (8:2 v /v), hexane: acetate (FHAEt) (6:4 v /v), acetate (FAE) (1:0 v /v), acetate: methanol (FAEM) (8:2 v /v), acetate: methanol (FAEMe) (7:3 v /v) and methanol (FM) (1:0 v /v) successively [ 18 ]. The partitions were concentrated under reduced pressure to dryness and stored at 4 °C.…”

Section: Methodsmentioning

confidence: 99%

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Phytochemical analysis and in vitro anthelmintic activity of Lophira lanceolata (Ochnaceae) on the bovine parasite Onchocerca ochengi and on drug resistant strains of the free-living nematode Caenorhabditis elegans

Kalmobé

Ndjonka

Djafsia

et al. 2017

BMC Complement Altern Med

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BackgroundOnchocerciasis is one of the tropical neglected diseases (NTDs) caused by the nematode Onchocerca volvulus. Control strategies currently in use rely on mass administration of ivermectin, which has marked activity against microfilariae. Furthermore, the development of resistance to ivermectin was observed. Since vaccine and safe macrofilaricidal treatment against onchocerciasis are still lacking, there is an urgent need to discover novel drugs. This study was undertaken to investigate the anthelmintic activity of Lophira lanceolata on the cattle parasite Onchocerca ochengi and the anthelmintic drug resistant strains of the free living nematode Caenorhabditis elegans and to determine the phytochemical profiles of the extracts and fractions of the plants.MethodsPlant was extracted in ethanol or methanol-methylene chloride. O. ochengi, C. elegans wild-type and C. elegans drug resistant strains were cultured in RPMI-1640 and NGM-agar respectively. Drugs diluted in dimethylsulphoxide/RPMI or M9-Buffer were added in assays and monitored at 48 h and 72 h. Worm viability was determined by using the MTT/formazan colorimetric method. Polyphenol, tannin and flavonoid contents were determined by dosage of gallic acid and rutin. Acute oral toxicity was evaluated using Swiss albino mice.ResultsEthanolic and methanolic-methylene chloride extracts killed O. ochengi with LC50 values of 9.76, 8.05, 6.39 μg/mL and 9.45, 7.95, 6.39 μg/mL respectively for leaves, trunk bark and root bark after 72 h. The lowest concentrations required to kill 50% of the wild-type of C. elegans were 1200 and 1890 μg/mL with ethanolic crude extract, 1000 and 2030 μg/mL with MeOH-CH2Cl2 for root bark and trunk bark of L. lanceolata, respectively after 72 h. Leave extracts of L. lanceolata are lethal to albendazole and ivermectin resistant strains of C. elegans after 72 h. Methanol/methylene chloride extracted more metabolites. Additionally, extracts could be considered relatively safe.ConclusionEthanolic and methanolic-methylene chloride crude extracts and fractions of L. lanceolata showed in vitro anthelmintic activity. The extracts and fractions contained polyphenols, tannins, flavonoids and saponins. The mechanism of action of this plant could be different from that of albendazole and ivermectin. These results confirm the use of L. lanceolata by traditional healers for the treatment of worm infections.Electronic supplementary materialThe online version of this article (doi:10.1186/s12906-017-1904-z) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Phytochemical analysis and in vitro anthelmintic activity of Lophira lanceolata (Ochnaceae) on the bovine parasite Onchocerca ochengi and on drug resistant strains of the free-living nematode Caenorhabditis elegans

Kalmobé

Ndjonka

Djafsia

et al. 2017

BMC Complement Altern Med

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“…After which the plates were observed for colony growth, the MBC/MFC was recorded as the lowest concentration of compounds showing no bacterial/fungal growth. 16…”

Section: Minimum Bactericidal/fungicidal Concentration (Mbc/mfc)mentioning

confidence: 99%

Synthesis and Antimicrobial Evaluation of N,N'-(4-Nitro-1,2-phenylene)diamide Derivatives

Iwuala¹,

Habila²,

Sallau³

2018

TJNPR

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medicinal chemists as amide derivatives are associated with broad spectrum of biological activities including anti-tuberculosis, 7 anticonvulsant, 8 analgesic-anti-inflammatory, 9 insecticidal, 10 antifungal, 11 and antitumor properties. 12 There is a need for discovery of new compounds which can be used clinically and has antibacterial and antifungal activities in which their mechanism of action are distinct from existing antimicrobial agents and do not show resistance compared to the existing antimicrobial agents. 13 Materials and Methods General experimental procedures All chemicals were of analytical grade and purchased from Sigma-Aldrich (Germany) through Bristol Scientific Company and commercial suppliers. Analytical thin layer chromatography (TLC) was performed using aluminum plates coated with silica gel 60F254 (Merck). Spots were visualized using UV lamp or by saturation with Iodine vapour. NMR spectra were recorded using 400 MHz spectrometer at room temperature in DMSO-d6. The infrared spectra of the solid samples were recorded on Agilent FTIR 400s Fourier Transform Infra-Red Spectrophotometer. Melting point determination was carried out on Stuart Automatic Melting Point /SMP40/ apparatus. Synthesis of N,N'-(4-Nitro-1,2-phenylene)diacetamide (3a) An intimate mixture of Acetic anhydride (0.04 mol), 4-Nitro-1,2phenylenediamine (0.02 mol) and 20 mL of toluene was stirred at room temperature for 20 minutes. The reaction was monitor with TLC until completion, the solvent was evaporated using rotary evaporator and the separated solid was filtered, washed with toluene and dried. The crude products were recrystallized from ethanol for further purification (Scheme 1).

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“…Ochna schweinfurthiana F. Hoffm is a tropical small tree that measures upto 4 metres and belongs to the Ochnaceae family. It is used by the population of North Cameroon to treat different metabolic diseases involving mechanisms of oxidation or inflammation such as rubella, burns, stomach ache, and multiple sclerosis [11]. Several studies revealed that the Ochnaceae family is a rich source of complex metabolites such as flavonoids, chalcones, steroids, terpenoids, and alkaloids [12–14].…”

Section: Introductionmentioning

confidence: 99%

Phytochemical Study of Aqueous Extract of Ochna schweinfurthiana F. Hoffm Powder Bark and Evaluation of Their Anti-Inflammatory, Cytotoxic, and Genotoxic Properties

Djova

Nyegue

Messi

et al. 2019

Evidence-Based Complementary and Alternative Medicine

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Ochna schweinfurthiana has been used in traditional medicine to treat pain, inflammation, and arthritis. It is a rich source of complex dimers of flavonoids with potential use as templates for the development of therapeutic drugs. Hence, the aim of this study was to study the phytochemical content and evaluate the in vitro cytotoxic, genotoxic, and anti-inflammatory activities of the aqueous extract of Ochna schweinfurthiana bark (OSE). Phytochemical study was carried out according to LC-MS procedures, while isolation was carried out using thin layer and column chromatographies. Cytotoxicity was investigated by the mitochondrial viability [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] (MTT) method while genotoxicity potential of the extract was ascertained using the Salmonella typhimurium test strains TA98 and TA100. The anti-inflammatory effect of OSE was evaluated by the in vitro inhibition of 15-lipooxygenase enzyme and bovine serum albumin denaturation (BSA) assays. The investigation of compounds extracted from OSE led to the identification and isolation of six known compounds, namely, hemerocallone (9), 6,7-dimethoxy-3’-4’-dimethoxyisoflavone (10), lithospermoside (13), amentoflavone (14), agathisflavone (15), and β-D-fructofuranosyl-α-D-glucopyranoside (17). In the anti-inflammatory assay, aqueous extracts of the bark showed selective inhibition of 15-lipooxygenase with IC50 value of 32.2±0.36 μg/mL and the result of the bovine serum albumin denaturation assay with IC50 value of 130± 5.78 μg/mL showed moderate activity. The toxicity assay indicated that OSE are noncytotoxic on Vero cell line with LC50 value of 50 mg/mL and nongenotoxic toward Salmonella typhimurium tester strain TA98 and TA100. Result from this study supports the traditional use of the selected medicinal plants in Cameroon for the treatment of inflammatory conditions. Noncytotoxicity and nongenotoxicity of OSE suggest that this plant is safe for use.

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Isolation and characterization of an anti-microbial biflavonoid from the chloroform-soluble fraction of methanolic root extract of Ochna schweinfurthiana (Ochnaceae)

Cited by 17 publications

References 21 publications

Phytochemical analysis and in vitro anthelmintic activity of Lophira lanceolata (Ochnaceae) on the bovine parasite Onchocerca ochengi and on drug resistant strains of the free-living nematode Caenorhabditis elegans

Phytochemical analysis and in vitro anthelmintic activity of Lophira lanceolata (Ochnaceae) on the bovine parasite Onchocerca ochengi and on drug resistant strains of the free-living nematode Caenorhabditis elegans

Synthesis and Antimicrobial Evaluation of N,N'-(4-Nitro-1,2-phenylene)diamide Derivatives

Phytochemical Study of Aqueous Extract of Ochna schweinfurthiana F. Hoffm Powder Bark and Evaluation of Their Anti-Inflammatory, Cytotoxic, and Genotoxic Properties

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