Isolation and characterization of an Aux/IAA gene (LaIAA2) from Larix

Feng, Jian; Liu, Qi; Sun, Xiaomei; Lin, Yongqi; Dong, Jian; Zhang, Shougong

doi:10.5897/ajb10.1009

Afr. J. Biotechnol.

2012

DOI: 10.5897/ajb10.1009

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Isolation and characterization of an Aux/IAA gene (LaIAA2) from Larix

Jian Feng¹,

Qi Liu²,

Xiaomei Sun³

et al.

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2020

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A full-length transcriptome and gene expression analysis reveal genes and molecular elements expressed during seed development in Gnetum luofuense

Deng¹,

Hou

et al. 2020

BMC Plant Biol

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Background Gnetum is an economically important tropical and subtropical gymnosperm genus with various dietary, industrial and medicinal uses. Many carbohydrates, proteins and fibers accumulate during the ripening of Gnetum seeds. However, the molecular mechanisms related to this process remain unknown. Results We therefore assembled a full-length transcriptome from immature and mature G. luofuense seeds using PacBio sequencing reads. We identified a total of 5726 novel genes, 9061 alternative splicing events, 3551 lncRNAs, 2160 transcription factors, and we found that 8512 genes possessed at least one poly(A) site. In addition, gene expression comparisons of six transcriptomes generated by Illumina sequencing showed that 14,323 genes were differentially expressed from an immature stage to a mature stage with 7891 genes upregulated and 6432 genes downregulated. The expression of 14 differentially expressed transcription factors from the MADS-box, Aux/IAA and bHLH families was validated by qRT-PCR, suggesting that they may have important roles in seed ripening of G. luofuense. Conclusions These findings provide a valuable molecular resource for understanding seed development of gymnosperms.

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A full-length transcriptome and gene expression analysis reveal genes and molecular elements expressed during seed development in Gnetum luofuense

Deng¹,

Hou

et al. 2020

BMC Plant Biol

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show abstract

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Isolation and characterization of an Aux/IAA gene (LaIAA2) from Larix

Cited by 1 publication

References 42 publications

A full-length transcriptome and gene expression analysis reveal genes and molecular elements expressed during seed development in Gnetum luofuense

A full-length transcriptome and gene expression analysis reveal genes and molecular elements expressed during seed development in Gnetum luofuense

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