Isolation and Characterization of an Invertase and Its Repressor Genes fromSchizosaccharomyces pombe

Tanaka, Naotaka; Ohuchi, Nobuhiro; Mukai, Yoshihiko; Ôsaka, Yukio; Ohtani, Yoshihiko; Tabuchi, Mitsuaki; Bhuiyan, M. Shah Alam; Fukui, Hiroshi; Harashima, Satoshi; Takegawa, Kaoru

doi:10.1006/bbrc.1998.8406

Cited by 63 publications

(64 citation statements)

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“…In this study, we found that gal10 + is efficiently expressed and involved in galactosylation of cell-surface proteins in cells grown in low (0.1 %) glucose medium, but not in galactose-containing medium (Figs 3b, 4 and 7a). We previously reported that the inv1 + gene that encodes invertase is repressed in the presence of glucose (Tanaka et al, 1998). The transcription of inv1 + is regulated by Scr1p, which is required for glucose repression of inv1 + (Tanaka et al, 1998) and fbp1 + (Neely & Hoffman, 2000).…”

Section: Discussionmentioning

confidence: 99%

“…We previously reported that the inv1 + gene that encodes invertase is repressed in the presence of glucose (Tanaka et al, 1998). The transcription of inv1 + is regulated by Scr1p, which is required for glucose repression of inv1 + (Tanaka et al, 1998) and fbp1 + (Neely & Hoffman, 2000). Similarly, Scr1p may have repressed the transcription of gal10 + in 2 % glucose medium.…”

Section: Discussionmentioning

confidence: 99%

“…In Sch. pombe, however, these control mechanisms have not been analysed in detail (Tanaka et al, 1998;Hoffman, 2005;Kig et al, 2005), especially for enzymes needed for utilization of galactose as an alternative carbon source. Interestingly, Sch.…”

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Characterization of two different types of UDP-glucose/-galactose4-epimerase involved in galactosylation in fission yeast

et al. 2010

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Schizosaccharomyces species are currently the only known organisms with two types of genes encoding UDP-glucose/-galactose 4-epimerase, uge1 + and gal10 + . A strain deleted for uge1 + exhibited a severe galactosylation defect and a decrease in activity and in UDP-galactose content when grown in glucose-rich medium (2 % glucose), indicating that Uge1p is a major UDPglucose/-galactose 4-epimerase under these growth conditions. In contrast, gal10 + was efficiently expressed and involved in galactosylation of cell-surface proteins in low-glucose medium (0.1 % glucose and 2 % glycerol), but not in galactose-containing medium. In a uge1Dgal10D strain, the galactosylation defect was suppressed and UDP-galactose content restored to wild-type levels in galactose-containing medium. Disruption of gal7 + , encoding galactose-1-phosphate uridylyltransferase, in the uge1Dgal10D strain reversed suppression of the galactosylation defect and reduced levels of UDP-galactose, indicating that galactose is transported from the medium to the cytosol and is converted into UDP-galactose via galactose 1-phosphate by Gal7p in Sch. pombe. INTRODUCTIONGlycoproteins in the fission yeast Schizosaccharomyces pombe contain a large amount of galactose in addition to both N-and O-linked mannan (Manners & Meyer, 1977;Moreno et al., 1985;Ikeda et al., 2009;Ohashi et al., 2009), indicating that Sch. pombe is equipped with mechanisms for glycoprotein galactosylation like animal cells. Research has demonstrated that the outer chain structure of galactomannan has an a-1,2-linked galactose or pyruvylated galactose attached to a poly-a-1,6-linked mannose backbone (Gemmill & Trimble, 1998), and that the pyruvylated galactose epitope bears remarkable structural resemblance to the N-acetylneuraminic acid-linked galactose epitope found in mammalian cells (Gemmill & Trimble, 1996). These galactose residues are enzymically modified by galactosyltransferases using UDP-galactose as substrate (Andreishcheva et al., 2004; Chappell et al., 1994;Yoko-o et al., 1998). UDP-galactose is transported to the Golgi lumen from the cytosol by the Golgi-localized UDPgalactose transporter (Gms1p) (Tabuchi et al., 1997;). In Sch. pombe, the precise manner in which UDP-galactose is synthesized in the cytosol has been unclear. In the cytosol, the key enzyme for UDP-galactose synthesis is UDP-galactose/-glucose 4-epimerase, which catalyses the interconversion of UDPgalactose and UDP-D-glucose. Interestingly, Schizosaccharomyces species have two types of UDP-glucose/-galactose 4-epimerase, while other organisms have only one. It is not known why two types of epimerase are needed and how they are regulated in Sch. pombe.In Saccharomyces cerevisiae, Gal10p is the sole UDPglucose/-galactose 4-epimerase, which consists of a UDPglucose/-galactose 4-epimerase domain and a galactose mutarotase domain (Majumdar et al., 2004;Scott & Timson, 2007;Thoden & Holden, 2005), and which catalyses mutarotation of a-galactose to b-galactose. Expression of the GAL10 gene is regulated by Gal4p, a DNA...

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Characterization of two different types of UDP-glucose/-galactose4-epimerase involved in galactosylation in fission yeast

et al. 2010

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“…Emericella nidulans (13), Aspergillus niger (14), A. oryzae (GenBank TM accession number AAK 11189), A. aculeatus (GenBank TM accession number BAA 75519), H. jecorina (15)(16)(17), Trichoderma harzianum (17), Metarhizium anisopliae (18), Botrytis cinerea (GenBank TM accession number Y16625), Cochliobolus carbonum (GenBank TM accession number AAG 29826), Neurospora crassa (GenBank TM accession number AF055464), Acremonium chrysogenum (19), Gibberella fujikuroi (GenBank TM accession number Y16626), Humicola grisea (20), and Sclerotinia sclerotiorum (21). It is intriguing that the respective gene products share a high degree of similarity with each other and with the Mig1 homologue from the fission yeast Schizosaccharomyces pombe (22) throughout several parts of the entire amino acid sequence, whereas a high degree of similarity with Mig1 is only apparent in the N-terminal DNA-binding zinc finger domain. As the Cre protein C-terminal part exhibits several features characteristic for transactivation domains (13,15) one may speculate that the regulation of Cre-mediated repression is different from that exerted by Mig1 in S. cerevisiae.…”

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Phosphorylation Positively Regulates DNA Binding of the Carbon Catabolite Repressor Cre1 of Hypocrea jecorina(Trichoderma reesei)

Cziferszky¹,

Mach²,

Kubicek

2002

Journal of Biological Chemistry

113

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Cre1 of the ascomyceteCarbon catabolite repression is a means by which cells manage priority use of easy and fast metabolizable carbon sources over more complex ones. In Saccharomyces cerevisiae, mutations in MIG1 or in respective binding sites in Mig1 regulated promoters partially relieve the yeast from carbon catabolite repression (1-4). To form an active repressor complex, Mig1 requires the co-repressors Ssn6 (Cyc8)-Tup1 (5-8). Mig1 function is regulated by glucose-dependent nuclear import/export, Mig1 being nuclear when glucose is present and being transported to the cytoplasm when glucose becomes limiting (9). Concomitantly, Mig1 has been shown to be phosphorylated under derepressing conditions (10, 11). Present genetic evidence suggests that both of these events are mediated by the Snf1 kinase complex: a mutation in MIG1 suppresses the snf1 mutant defects in SUC2 and GAL1 expression (3, 4), indicating that SNF1 negatively regulates repression by Mig1. Furthermore, Mig1 is permanently localized in the nucleus in snf1

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“…inv1 ϩ is transcriptionally controlled by the zinc finger transcription repressor Scr1 (Fig. 3A) (34). The subcellular localization of Scr1 is regulated, and the protein is quickly exported from the nucleus to the cytoplasm immediately after glucose starvation.…”

Section: Med15 But Not Hrp1 Is Required For Transcriptional Activatmentioning

confidence: 99%

A Chromatin-remodeling Protein Is a Component of Fission Yeast Mediator

Khorosjutina

Wanrooij

Walfridsson

et al. 2010

Journal of Biological Chemistry

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Isolation and Characterization of an Invertase and Its Repressor Genes fromSchizosaccharomyces pombe

Cited by 63 publications

References 45 publications

Characterization of two different types of UDP-glucose/-galactose4-epimerase involved in galactosylation in fission yeast

Characterization of two different types of UDP-glucose/-galactose4-epimerase involved in galactosylation in fission yeast

Phosphorylation Positively Regulates DNA Binding of the Carbon Catabolite Repressor Cre1 of Hypocrea jecorina(Trichoderma reesei)

A Chromatin-remodeling Protein Is a Component of Fission Yeast Mediator

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