Isolation and characterization of aerobic culturable arsenic-resistant bacteria from surfacewater and groundwater of Rautahat District, Nepal

Shakya, Siddhartha; Pradhan, B.; Smith, Linda S.; Shrestha, Jayana N; Tuladhar, Sampurna

doi:10.1016/j.jenvman.2011.08.001

Cited by 42 publications

(33 citation statements)

References 19 publications

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“…Screening for As (III) oxidation and As (V) reduction activity The bacteria were tested on the ability to oxidize As (III) or reduce As (V) using a qualitative AgNO 3 screening method [10,11]. The bacteria were grown on nutrient broth with either 129.91 mg/L NaAsO 2 (sodium arsenite) or 312.01 mg/L Na 2 HAsO 4 (sodium arsenate).…”

Section: Sensitivity To Antibioticsmentioning

confidence: 99%

“…E. coli grow at 0.01 mM mercury concentration (a), 1 mM zinc concentration (b), and 1 mM nickel (c). Screening for As (III) oxidation and As (V) reduction activity The bacteria were tested on the ability to oxidize As (III) or reduce As (V) using a qualitative AgNO 3 screening method [10,11]. A light yellow color in mixtures containing arsenate indicates positive arsenate oxidation reaction (oxidation of the arsenate in the mixture to arsenite).…”

Section: Sensitivity To Antibioticsmentioning

confidence: 99%

“…This means that the arsenate supplied was transformed into arsenite. Although absence of any brown coloring shows that arsenite was not transformed to arsenate [10,11].…”

Section: Sensitivity To Antibioticsmentioning

confidence: 99%

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Isolation of Arsenic Resistant Escherichia coli from Sewage Water and Its Potential in Arsenic Biotransformation

Bista

Shakya

2017

JTLS

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Arsenic contamination in drinking water from ground water poses a threat to the health of a large population in developing countries in Asia. This has sparked great interests in the potential of different microbes in arsenic resistance and removal from water. This study involves isolation of arsenic resistant Escherichia coli from sewage water from Kathmandu University and investigation of its attributes. Arsenic resistant E. coli was successfully isolated which could survive in high concentration of arsenic. The maximum tolerance of arsenite was 909.79 mg/L (sodium arsenite) and 3120.1 mg/L arsenate (sodium arsenate) which is well above most natural concentration of arsenic in ground water. This particular E. coli tolerated multiple heavy metal like silver nitrate, cobalt sulphate, cadmium chloride, nickel chloride, mercury chloride, copper sulphate, and zinc chloride at concentration 20 µM, 1 mM, 0.5mM, 1mM, 0.01 mM, 1 mM, and 1 mM respectively which are concentrations known to be toxic to E. coli. Biotransformation of arsenite to arsenate was also checked for by a qualitative silver nitrate technique. This E. coli was able to transform arsenate to arsenite. It showed some sensitivity to Ciprofloxacin, Gentamicin and Nalidixic Acid. As E. coli and its genome are very widely studied, these particular properties have a lot of potential in microbial remediation or microbial recovery of metals and possible recombination approaches.

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Section: Sensitivity To Antibioticsmentioning

confidence: 99%

Section: Sensitivity To Antibioticsmentioning

confidence: 99%

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Isolation of Arsenic Resistant Escherichia coli from Sewage Water and Its Potential in Arsenic Biotransformation

Bista

Shakya

2017

JTLS

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“…Finally, the pellet was air dried for a day in 25 o C, treated with 500 μl of conc. HNO 3 and Unauthenticated Download Date | 5/13/18 4:57 AM bioaccumulation of arsenic in cell mass was estimated using atomic absorption spectrophotometry (AAS, Philips model PU 9100) 5 .…”

Section: Bioaccumulation Assaymentioning

confidence: 99%

“…Arsenate generally is the dominant form in oxic waters, while arsenite dominates in sulfi dic and methanic waters including most geothermal water. Both forms are toxic; comparatively arsenite is the most toxic form 4, 5 . Chronic arsenic poisoning in the general population has been widely reported in many areas of the world today 9 .…”

Section: Introductionmentioning

confidence: 99%

Optimal conditions for the biological removal of arsenic by a novel halophilic archaea in different conditions and its process optimization

Taran¹,

Safari²,

Monaza³

et al. 2013

Polish Journal of Chemical Technology

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Recently, concerns about arsenic have been increased due to its high acute toxicity to human and serious environmental problems. In this study, the ability of Halorcula sp. IRU1, a novel halophilic archaea isolated from Urmia lake, Iran for arsenic bioaccumulation was investigated and optimized by Taguchi experimental design. The optimum conditions for high arsenic bioaccumulation by Haloarcula sp. IRU1 could be achieved in the presence temperature 40 o C, pH 8 and NaAsO 2 at 90 mg/L. Under optimum conditions, the microorganism was able to perform their desired function with a 60.89 percent removal of arsenic. In conclusion, Haloarcula sp. IRU1 is resistant to arsenic and removes it in different conditions.

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Bioremediation of Water: A Sustainable Approach

Chakraborty

Sikder

Mukherjee

et al. 2013

Sustainable Development in Chemical Engineering Innovative Technologies

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Isolation and characterization of aerobic culturable arsenic-resistant bacteria from surfacewater and groundwater of Rautahat District, Nepal

Cited by 42 publications

References 19 publications

Isolation of Arsenic Resistant Escherichia coli from Sewage Water and Its Potential in Arsenic Biotransformation

Isolation of Arsenic Resistant Escherichia coli from Sewage Water and Its Potential in Arsenic Biotransformation

Optimal conditions for the biological removal of arsenic by a novel halophilic archaea in different conditions and its process optimization

Bioremediation of Water: A Sustainable Approach

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