Isolation and characterization of acidophilic bacteria from Patagonia, Argentina

Lavalle, L.; Chiacchiarini, P.; Pogliani, Cristina; Donati, Edgardo R.

doi:10.1016/j.procbio.2004.03.008

Cited by 27 publications

(17 citation statements)

References 15 publications

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“…Our research group has been working on the assessment of the biodiversity of Río Agrio for the last 20 years. At the beginning, we relied only on culture depending techniques which allowed the identification of several acidophilic iron and/or sulfur oxidizing species [32,33]. These results encourage us to perform more comprehensive assessments of prokaryotic biodiversity, such as cloning and sequencing of 16S rRNA genes of bacteria and archaea [10].…”

Section: Characteristics Of the Prokaryotic Community Of The Copahue mentioning

confidence: 99%

A Deeper Look into the Biodiversity of the Extremely Acidic Copahue volcano-Río Agrio System in Neuquén, Argentina

et al. 2019

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The Copahue volcano-Río Agrio system, on Patagonia Argentina, comprises the naturally acidic river Río Agrio, that runs from a few meters down the Copahue volcano crater to more than 40 km maintaining low pH waters, and the acidic lagoon that sporadically forms on the crater of the volcano, which is studied for the first time in this work. We used next-generation sequencing of the 16S rRNA gene of the entire prokaryotic community to study the biodiversity of this poorly explored extreme environment. The correlation of the operational taxonomic units (OTUs)s presence with physicochemical variables showed that the system contains three distinct environments: the crater lagoon, the Upper Río Agrio, and the Salto del Agrio waterfall, a point located approximately 12 km down the origin of the river, after it emerges from the Caviahue lake. The prokaryotic community of the Copahue Volcano-Río Agrio system is mainly formed by acidic bacteria and archaea, such as Acidithiobacillus, Ferroplasma, and Leptospirillum, which have been isolated from similar environments around the world. These results support the idea of a ubiquitous acidic biodiversity; however, this highly interesting extreme environment also has apparently autochthonous species such as Sulfuriferula, Acidianus copahuensis, and strains of Acidibacillus and Alicyclobacillus.

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Section: Characteristics Of the Prokaryotic Community Of The Copahue mentioning

confidence: 99%

A Deeper Look into the Biodiversity of the Extremely Acidic Copahue volcano-Río Agrio System in Neuquén, Argentina

et al. 2019

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“…At Río Tinto, which presents iron concentrations around 1,000 mg/l, Nitrospirae is one of the most abundant phylogenetic groups (up to 65%), together with Gammaproteobacteria [24]. Strains of Acidithiobacillus ferrooxidans, Acidithiobacillus thiooxidans and L. ferrooxidans not only have been detected by molecular biology techniques, but also have been isolated from different points of Río Agrio on 9K-agarose solid medium [35]. As for Betaproteobacteria, although not many species were detected by cloning and sequencing, it is worth noticing that they appeared downstream, as physicochemical conditions became less extreme.…”

Section: Discussionmentioning

confidence: 99%

“…Studies have also been done on its microbiology, mainly focusing on characterisation and isolation of native acidophilic microorganisms [11,35] and their use in specific processes [21], but its whole prokaryotic biodiversity has never been studied and reported before. Revealing this particular natural environment prokaryotic biodiversity by non-culture techniques may improve detection and isolation of microorganisms involved in iron and sulphur cycles that can be useful in biomining and bioremediation processes.…”

Section: Introductionmentioning

confidence: 99%

First Prokaryotic Biodiversity Assessment Using Molecular Techniques of an Acidic River in Neuquén, Argentina

et al. 2012

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Two acidic hot springs close to the crater of Copahue Volcano (Neuquén, Argentina) are the source of the Río Agrio. The river runs several kilometres before flowing into Caviahue Lake. Along the river, temperature, iron, other metal and proton concentrations decrease gradually with distance downstream. From the source to the lake and depending on the season, pH can rise from 1.0 (or even less) to about 4.0, while temperature values decrease from 70°C to 15°C. Water samples were taken from different stations on the river selected according to their physicochemical parameters. In order to assess prokaryotic biodiversity throughout the water column, different and complementary molecular biology techniques were used, mainly in situ hybridisation and 16S rRNA gene cloning and sequencing. All microorganisms found are typical of acidic environments. Sulphur-oxidizing bacteria like Acidithiobacillus thiooxidans and Acidithiobacillus albertensis were detected in every station. Moderately thermophile iron- and sulphur-oxidizing bacteria like members of Alicyclobacillus and Sulfobacillus genera were also ubiquitous. Strict iron-oxidizing bacteria like Leptospirillum and Ferrimicrobium were present at the source of the river, but disappeared downstream where iron concentrations were much lower. Iron-oxidizing, mesophilic Ferroplasma spp. were the main archaea found. The data presented in this work represent the first molecular assessment of this rare natural acidic environment.

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“…As bactérias nativas oxidantes de íon ferroso que participam na biolixiviação de minérios sulfatados são microrganismos que estão constantemente expostos a essas mudanças e podem apresentar vantagens sobre as bactérias comumente utilizadas na biolixiviação devido a que estão adaptadas a composição mineralógica e química dos minérios a serem tratados. Essas vantagens poderiam levar a um aumento nos valores de extração do metal durante o processo de biolixiviação [6]. Neste trabalho foram coletas amostras liquidas de drenagem acida de Mina (DAM) no distrito de Palca (Peru) e as mesmas utilizadas para isolar microrganismos com potencial para a oxidação de íon ferroso.…”

Section: Introductionunclassified

Isolamento De Bactérias Nativas Heterótrofas Oxidantes De Ferro De Uma Mina Abandonada De Cobre Em Palca (Peru) E Identificação Por Maldi-Tof

Moreno¹,

Espinosa²,

Sígolo³

2018

ABM Proceedings

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Iron oxidizing bacteria indigenous play a major role in the bioleaching processes of copper sulfides ores. The use do these bacteria could improve bioleaching process due to be adapted to the environmental conditions and the levels of metals present in the ore. The aim of this study was to isolate and identify ironoxidizing heterotrophic bacteria indigenous of acid mine drainage (AMD) from an abandoned copper mine located in Palca, Peru. Samples of AMD were enriched in FeTo cultivation medium and an inoculum was plated to obtain pure lines. In this study, seven bacterial strain iron-oxidizing were isolated and your cell morphology was observed by scanning electron microscope (SEM). Oxidation of ferrous iron by bacteria strain was studied qualitatively and detected by change in medium color. MALDI-TOF was used for the bacterial identification and allowed separating the bacterial strains according to their protein profile.

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Isolation and characterization of acidophilic bacteria from Patagonia, Argentina

Cited by 27 publications

References 15 publications

A Deeper Look into the Biodiversity of the Extremely Acidic Copahue volcano-Río Agrio System in Neuquén, Argentina

A Deeper Look into the Biodiversity of the Extremely Acidic Copahue volcano-Río Agrio System in Neuquén, Argentina

First Prokaryotic Biodiversity Assessment Using Molecular Techniques of an Acidic River in Neuquén, Argentina

Isolamento De Bactérias Nativas Heterótrofas Oxidantes De Ferro De Uma Mina Abandonada De Cobre Em Palca (Peru) E Identificação Por Maldi-Tof

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