Isolation and characterization of Achromobacter sp. CX2 from symbiotic Cytophagales, a non-cellulolytic bacterium showing synergism with cellulolytic microbes by producing β-glucosidase

Chen, Xiaoyi; Wang, Ying; Yang, Fan; Qu, Yinbo; Li, Xianzhen

doi:10.1007/s13213-014-1009-6

Cited by 11 publications

(7 citation statements)

References 32 publications

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“…In vermicompost samples, 11.9–45.9% of the Bacteroidetes sequences were assigned to the genus Cytophagaceae_uncultured (order Cytophagales), which are cellulolytic [ 40 ]. The relative abundance of Bacteroidetes in aerobic compost samples increased with composting time from 30 d to 45 d and from 90 d to 150 d. The relative abundance of Cytophagaceae_uncultured was lower in aerobic compost (1.00–2.66%) than in vermicompost.…”

Section: Resultsmentioning

confidence: 99%

Comparison of chemical and microbiological changes during the aerobic composting and vermicomposting of green waste

et al. 2018

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This research was conducted to compare chemical and microbiological properties during aerobic composting (AC) and vermicomposting (VC) of green waste. Relative to AC, VC significantly decreased the pH and lignin and cellulose contents, and significantly increased the electrical conductivity and total N and available P contents. For AC, BIrii41_norank (order Myxococcales) was the major bacterial genus at 30 d and again became dominant genus from 90–150 d, with relative abundances of 2.88% and 4.77–5.19%, respectively; at 45 d and 60 d, the dominant bacterial genus was Nitrosomonadaceae_uncultured (order Nitrosomonadales) with relative abundances of 2.83–7.17%. For VC, the dominant bacterial genus was BIrii41_norank (except at 45 d), which accounted for 2.11–7.96% of the total reads. The dominant fungal class was Sordariomycetes in AC (relative abundances 39.2–80.6%) and VC (relative abundances 42.1–69.5%). The abundances of microbial taxa and therefore the bacterial and fungal community structures differed between VC and AC. The quality of the green waste compost product was higher with VC than with AC. These results will also help to achieve further composting technology breakthroughs in reducing the composting time and improving compost quality.

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Section: Resultsmentioning

confidence: 99%

Comparison of chemical and microbiological changes during the aerobic composting and vermicomposting of green waste

et al. 2018

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“…Specific mucolytic enzymes, such as glycosidase, sulfatase, and sialidase enzymes, were found in bacteria spanning several genera [41][42][43][44][45]. Strikingly, Chen et al previously isolated a novel species of Achromobacter that could produce βglucosidase [46,47], implicating the potential mechanism of A. pulmonis in mucous impairment. Genomic characterization of our A. pulmonis isolate will further help understand the mucolytic capacity of this bacterium.…”

Section: Discussionmentioning

confidence: 99%

Microbiota in mesenteric adipose tissue from Crohn’s disease promote colitis in mice

et al. 2021

Microbiome

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Background Mesenteric adipose tissue (mAT) hyperplasia, known as creeping fat is a pathologic characteristic of Crohn’s disease (CD). The reserve of creeping fat in surgery is associated with poor prognosis of CD patients, but the mechanism remains unknown. Methods Mesenteric microbiome, metabolome, and host transcriptome were characterized using a cohort of 48 patients with CD and 16 non-CD controls. Multidimensional data including 16S ribosomal RNA gene sequencing (16S rRNA), host RNA sequencing, and metabolome were integrated to reveal network interaction. Mesenteric resident bacteria were isolated from mAT and functionally investigated both in the dextran sulfate sodium (DSS) model and in the Il10 gene-deficient (Il10−/−) mouse colitis model to validate their pro-inflammatory roles. Results Mesenteric microbiota contributed to aberrant metabolites production and transcripts in mATs from patients with CD. The presence of mAT resident microbiota was associated with the development of CD. Achromobacter pulmonis (A. pulmonis) isolated from CD mAT could translocate to mAT and exacerbate both DSS-induced and Il10 gene-deficient (Il10−/−) spontaneous colitis in mice. The levels of A. pulmonis in both mAT and mucous layer from CD patients were higher compared to those from the non-CD group. Conclusions This study suggests that the mesenteric microbiota from patients with CD sculpt a detrimental microenvironment and promote intestinal inflammation.

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“…Strains of the genus Brevundimonas are opportunistic pathogens (Berg et al, 2009) and known to be involved in synergistic interactions with Anabaena and Ochrobactrum in plant growth promotion (Rana et al, 2015). Chen et al (2014) have shown the synergistic association of noncellulolytic Achromobacter sp. CX2 with cellulose-digesting Cytophagales producing β-glucosidase.…”

Section: O N L I N E C O P Ymentioning

confidence: 99%

Isolation of agarose digesting Achromobacter sp. and Brevundimonas diminuta using customized earthworm gut as an enrichment system

Pawar¹,

Mulye²,

Talker³

et al. 2023

JEB

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Aim: To isolate potential agarose digesting bacteria from gut of Eisenia fetida fed with agar agar as a major feed. Methodology: E. fetida was fed on agar agar for 17 days in a comprehensive vermicompost unit, and gut-washing was collected. A specially designed dilute nutrient medium containing agarose as a solidifying agent was used to grow and isolate agarose digesting bacteria. Agarose digestion was confirmed by iodine test and novel 'micro-tip’ assay. Results: A selected CFU comprising two bacterial species showed depressions on the media, indicative of agarose digestion. Subsequently, cloning based 16S rRNA gene identification revealed the two isolates to be Brevundimonas diminuta and Achromobacter sp. with 99% similarity. These two could not be separated as pure cultures, indicating a likely associative interaction. Interpretation: The present study is the first of its sort to demonstrate a probable associative interaction between Brevundimonas diminuta and Achromobacter sp. in agarose digestion, while using "Customized Earthworm Gut Enrichment System." Key words: Agarose digesters, Earthworm, Enrichment system, Gut microflora

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Isolation and characterization of Achromobacter sp. CX2 from symbiotic Cytophagales, a non-cellulolytic bacterium showing synergism with cellulolytic microbes by producing β-glucosidase

Cited by 11 publications

References 32 publications

Comparison of chemical and microbiological changes during the aerobic composting and vermicomposting of green waste

Comparison of chemical and microbiological changes during the aerobic composting and vermicomposting of green waste

Microbiota in mesenteric adipose tissue from Crohn’s disease promote colitis in mice

Isolation of agarose digesting Achromobacter sp. and Brevundimonas diminuta using customized earthworm gut as an enrichment system

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