Isolation and Characterization of a Novel SOMATIC EMBRYOGENESIS RECEPTOR KINASE Gene Expressed in the Fern Adiantum capillus-veneris During Shoot Regeneration In Vitro

Li, Xia; Fang, Yuhan; Han, Jing-Dan; Bai, Shu-Nong; Rao, Guang‐Yuan

doi:10.1007/s11105-014-0769-2

Cited by 18 publications

(8 citation statements)

References 45 publications

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“…Then, an excessive cell division produced GGB with a large number of meristematic cells, suggesting that the meristematic cells originated GGB in D. roosii. Although GGB can be successfully induced on different explants in several fern species [9,11,14,15], the precise cell origin of GGB has not been addressed. We believe this cognition was developed largely since the explants used for GGB induction already had meristematic cells.…”

Section: Discussionmentioning

confidence: 99%

“…However, none of these results were found during the process of GGB formation on rhizome explants, suggesting that the formation of GGB on rhizome explants in D. roosii under our induction condition did not involve the somatic embryogenesis pathway. Recently, the marker gene that is expressed during plant somatic embryogenesis, SERK, was identi ed in GGBs of the fern Adiantum capillus-veneris [15,41], suggesting that the development of GGB shares similar characteristics of that of SE. However, the SERK gene is not unique to the somatic embryogenesis pathway, and it was also expressed during the organogenesis pathway in some plant species [42][43][44][45].…”

Section: Similarities and Distinctions Between Ggb And Other Propagules Ggb Vs Sementioning

confidence: 99%

“…Liao and Wu [14] found that GGBs of Platycerium bifurcatum could differentiate into numerous new shoots following a process similar to organogenesis of callus. However, because the SOMATIC EMBRYOGENESIS RECEPTOR KINASE (SERK) gene, which is used as a marker to screen somatic cells that are capable of forming embryos, was expressed in the GGB of Adiantum capillus-veneris, Li et al [15] suggested that GGB development followed the somatic embryogenesis pathway. In Cibotium barometz, GGBs were compared to protocorm-like bodies (PLBs) of orchids et al [11].…”

Section: Introductionmentioning

confidence: 99%

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Green Globular Body (GGB) Induction and Differentiation in the Medicinal fern Drynaria Roosii

Yang

Dong

et al. 2021

Preprint

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Background: The green globular body (GGB) of ferns is a special propagule induced in plant in vitro culture systems. Owing to its high proliferation efficiency, GGB is widely used in the in vitro propagation of important ornamental and medicinal ferns. In addition, propagation using GGB shows great development prospects in the conservation of rare or endangered ferns and the breeding of new fern varieties. However, due to the lack of systematic studies on GGB ontogenesis, the morphogenetic aspects of GGB during induction and differentiation remain unclear.Results: We characterized the response of five types of explants of Drynaria roosii to GGB inductive medium and further investigate morphological and anatomical changes of explants that developed GGBs. We found that the rhizome explants directly produced GGBs through cell proliferation of the shoot apical meristem and lateral meristem. The leaf and petiole explants produced GGBs indirectly through the proliferation of meristematic cells of somatic embryos derived from the epidermal cells of the explants. The root and gametophyte explants failed to produce GGB under our induction conditions. We further investigated the differentiation process of GGB. During GGB differentiation, shoot primordia and leaf primordia differentiate from meristematic cells on the epidermis, and the root primordia develop from an inner meristematic tissue with developing vascular tissue connecting all these primordia, which indicates the involvement of multiple organogenesis processes.Conclusions: Our results suggested that preexisting or reestablished meristematic cells were the direct source of GGB in D. roosii. Somatic embryogenesis and organogenesis were involved in GGB induction and differentiation, respectively. The comparison with other common propagules revealed that GGB in D. roosii was largely different from somatic embryos, callus, and protocorm or protocorm-like bodies.

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Section: Discussionmentioning

confidence: 99%

Section: Similarities and Distinctions Between Ggb And Other Propagules Ggb Vs Sementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Green Globular Body (GGB) Induction and Differentiation in the Medicinal fern Drynaria Roosii

Yang

Dong

et al. 2021

Preprint

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“…GGBs are unique tissue emerging from explants in the tissue culture of pteridophytes. They are usually induced with runner tips, leaves and leaf primordia of sporophytes as explants In vitro (Bertrand et al, 1999;Higuchi et al, 1987;Liao and Wu, 2011;Li et al, 2015;Yu et al, 2017;Yu et al, 2021). GGBs exhibit great potential to generate many sporophytes due to the presence of several meristems within a single GGB.…”

Section: Introductionmentioning

confidence: 99%

Green Globular Body Induction and Plantlet Regeneration of Endangered Fern Adiantum reniforme var. sinense

Lin,

Shih,

Chang

et al. 2024

IJARe

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Background: Adiantum reniforme var. sinense is a pteridophyte with considerable ornamental importance and medicinal value. However, the wild population of A. reniforme var. sinense has gradually become scarce due to sparse distribution and human activities. Green Globular Bodies (GGBs) are specific tissue which use to propagate ferns in vitro. The study uses GGBs induced from sporophytic embryos to preserve and to proliferate this pteridophyte. Methods: The present study was carried out during year 2018-2020 at National Ilan University. The developing sporophyte of Adiantum reniforme var. sinense were used to induce GGBs. The optimal medium for GGBs proliferation and differentiation in terms of basal salts, plant growth regulators, sugar concentrations and gelling agents was examined. The structure of GGBs and the embryos were analyzed by paraffin section as well. Result: The result indicates the 100% GGBs induction rate from sporophytic embryos for the first time could be obtained under BA (0.1 mg L-1) and NAA (0.15 mg L-1). For subsequent proliferation of GGBs required a higher concentration of NAA (10 mg L-1). Cytokinins such BA, KT and TDZ, inhibited GGBs proliferation. In vitro optimal amount of basal salts, plant growth regulators, sugar concentrations and gelling agents for GGB proliferation were obtained as 2 g L-1 Hyponex, 10 mg L-1 NAA, 15 g L-1 sucrose and 4 g L-1 gelrite, respectively. Paraffin sections revealed histological characteristics of the sporophytic embryo and GGB.

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“…Compared with the conventional propagation via spore culture, in vitro propagation via GGB system is more e cient, for example 160,000 plantlets of N. cordifolia could be obtained from one runner tips via GGB system in 6 months (Higuchi et al 1987). GGBs, a special structure of ferns that is different from callus, is used not only for in vitro propagation, but also for cryopreservation (Pence 2015) and somatic embryogenesis (Li et al 2015).…”

Section: Introductionmentioning

confidence: 99%

In vitro propagation and long-term observation of acclimated plants in endangered tree fern Alsophila costularis

Song

Wang

et al. 2022

Plant Cell Tiss Organ Cult

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Alsophila costularis Barker (Cyathea costularis), an endangered tree fern with tree-like erect stem, attracts gardening enthusiasts as a special ornamental plant. In vitro propagation can be advantageous for germplasm conservation and commercial application of A. costularis. Here, we described in vitro propagation of A. costularis via spore culture and green globular bodies (GGBs) system, as well as the long-term observation of acclimated plants regenerated from GGBs. In spore culture, the low concentration of mineral salt (1/8 MS) was bene cial for sporophyte formation on gametophytes, but sporophytes per conical ask was only 8 plantlets. In GGB system, cytokinin thidiazuron (TDZ) was essential for GGB induction and multiplication. The maximum of GGB induction frequency (93.33%) was obtained on 1/2MS medium with 2.0 mg/l TDZ by using juvenile sporophytes as explants, and the same medium was optimal for GGB multiplication. 1/4 MS supplemented with 0.1% (w/v) activated carbon (AC) was appropriate for plantlet regeneration from GGB, GGB differentiation frequency was 100%, and 42.40 plantlets could be regenerated from one piece of GGBs. The maximum of plantlet height (4.64 cm) was obtained on 1/2 MS with 0.1% (w/v) AC. After 6 years of acclimatization cultivation for plantlets regenerated from GGBs, plants in the plastic pots with diameter of 60 cm showed the excellent vegetative and reproductive growth, and the mature spores of these plants could produce sporophytes. Morphological and histological observation demonstrated that A. costularis GGBs was a green structure consisting of multiple single GGBs with hair-like structures. One single GGB could develop into one plantlet.* Plantlet regeneration e ciency indicates the number of plantlets regenerated from one piece of GGBs, and the initial diameter and fresh weight of GGBs was 3.0 mm and 5.0 mg, respectively.

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Isolation and Characterization of a Novel SOMATIC EMBRYOGENESIS RECEPTOR KINASE Gene Expressed in the Fern Adiantum capillus-veneris During Shoot Regeneration In Vitro

Cited by 18 publications

References 45 publications

Green Globular Body (GGB) Induction and Differentiation in the Medicinal fern Drynaria Roosii

Green Globular Body (GGB) Induction and Differentiation in the Medicinal fern Drynaria Roosii

Green Globular Body Induction and Plantlet Regeneration of Endangered Fern Adiantum reniforme var. sinense

In vitro propagation and long-term observation of acclimated plants in endangered tree fern Alsophila costularis

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