Isolation and characterization of a new bacterium capable of biotransformingcis-epoxysuccinic acid to d(−)-tartaric acid

Abstract

A bacterial strain 1-3 capable of enantioselectively hydrolyzing cis-epoxysuccinic acid to D(-)-tartaric acid was isolated from soil. Phenotypic characteristics, Biolog GN test and phylogenetic analysis of strain 1-3 indicated that it belongs to the genus Bordetella. cis-Epoxysuccinic acid could be used as a carbon source and inducer for production of cis-epoxysuccinic acid hydrolase. After the purification of the enzymatic biotransformed product, a colorless and scentless crystal was obtained, which was confirmed to be the tartaric acid with laevorotatory optical rotation by the (1)H nuclear magnetic resonance spectrum and optical rotation analysis.

Enantiomeric Tartaric Acid Production Using cis-Epoxysuccinate Hydrolase: History and Perspectives

“…The microbial production of L(+)-tartaric acid was successfully commercialized in the late 1990s [32]. Subsequently, new bacterial species were isolated for TA production in the 21st century [18,33,34,35,36,37,38,39], which demonstrates that CESHs exist broadly in bacteria.…”

“…strain 1-3 and Bordetella sp. BK-52), isolated from vegetable fields in Hangzhou, were able to transform cis -epoxysuccinate to d (−)-TA [35,36]. Unlike traditional Bordetella species that are exclusively associated with humans and warm-blooded animals, both of these strains are from the natural environment.…”

Production of a newly isolated Paenibacillus polymyxa biocontrol agent using monosodium glutamate wastewater and potato wastewater

“…Alignments were then performed against the 16S rRNA gene sequence of isolate EBL-06, where the pair-wise deletion option for gaps was employed. The alignment data were then used for neighbour-joining analysis with 1000 bootstrap replicates (MEGA version 4.0; Arizona State University, USA) (Li et al, 2007).…”

Utilization of Sweat Potato Starch Wastewater and Monosodium Glutamate Wastewater for Cultivation of an Anti-Fungal Biocontrol Agent Paenibacillus Polymyxa

“…Alignments were then performed against the 16S rRNA gene sequence of isolate EBL06, where the pair-wise deletion option for gaps was employed. The alignment data were then used for neighbour-joining analysis with 1000 bootstrap replicates (MEGA version 4.0; Arizona State University, USA) (Li et al, 2007).…”