Isolation and characterization of a fluorene-degrading bacterium: identification of ring oxidation and ring fission products

Grifoll, Magdalena; Casellas, M.; Bayona, Josep M.; Solanas, Anna M.

doi:10.1128/aem.58.9.2910-2917.1992

Cited by 90 publications

(43 citation statements)

References 34 publications

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“…Casellas et al [16,17] identified new metabolites and demonstrated key enzyme activities in fluorene metabolism by Arthrobacter sp. strain F101, and proposed a novel metabolic pathway for fluorene.…”

Section: Discussionmentioning

confidence: 99%

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3,4‐Dihydrocoumarin hydrolase with haloperoxidase activity from Acinetobacter calcoaceticus F46

Kataoka

Honda

Shimizu

2000

European Journal of Biochemistry

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A novel lactonohydrolase, an enzyme that catalyzes the hydrolysis of 3,4-dihydrocoumarin, was purified 375-fold to apparent homogeneity, with a 22.7% overall recovery, from Acinetobacter calcoaceticus F46, which was isolated as a fluorene-assimilating micro-organism. The molecular mass of the native enzyme, as estimated by high-performance gel-permeation chromatography, is 56 kDa, and the subunit molecular mass is 30 kDa. The enzyme specifically hydrolyzes 3,4-dihydrocoumarin, and the Km and Vmax for 3,4-dihydrocoumarin are 0.806 mM and 4760 U.mg-1, respectively. The N-terminal and internal amino acid sequences of the enzyme show high similarity to those of bacterial non-heme haloperoxidases. The enzyme exhibits brominating activity with monochlorodimedon in the presence of H2O2 and 3, 4-dihydrocoumarin or an organic acid, such as acetate and n-butyrate.

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Section: Discussionmentioning

confidence: 99%

“…Casellas et al [16,17] demonstrated a novel metabolic pathway for fluorene (o-biphenylenemethane), a tricyclic aromatic compound containing a five-membered ring, in Arthrobacter sp. In this pathway, initial dioxygenation may occur at either positions C-1, C-2 or C-3, C-4 of fluorene.…”

mentioning

confidence: 99%

3,4‐Dihydrocoumarin hydrolase with haloperoxidase activity from Acinetobacter calcoaceticus F46

Kataoka

Honda

Shimizu

2000

European Journal of Biochemistry

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“…Fluorene is a major component of fossil fuels and can be commonly identified in the atmosphere, fresh water, and both riverine and marine sediments (Grifoll et al, 1992(Grifoll et al, , 1994(Grifoll et al, , 1995. Three major degradative pathways for fluorine have been proposed (Casellas et al, 1997(Casellas et al, , 1998Wattiau et al, 2001;Habe et al, 2004).…”

Section: Low-mw Pah Dioxygenase Systemmentioning

confidence: 99%

Microbial biodegradation of polyaromatic hydrocarbons

et al. 2008

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Polycyclic aromatic hydrocarbons (PAHs) are widespread in various ecosystems and are pollutants of great concern due to their potential toxicity, mutagenicity and carcinogenicity. Because of their hydrophobic nature, most PAHs bind to particulates in soil and sediments, rendering them less available for biological uptake. Microbial degradation represents the major mechanism responsible for the ecological recovery of PAH-contaminated sites. The goal of this review is to provide an outline of the current knowledge of microbial PAH catabolism. In the past decade, the genetic regulation of the pathway involved in naphthalene degradation by different gram-negative and gram-positive bacteria was studied in great detail. Based on both genomic and proteomic data, a deeper understanding of some high-molecular-weight PAH degradation pathways in bacteria was provided. The ability of nonligninolytic and ligninolytic fungi to transform or metabolize PAH pollutants has received considerable attention, and the biochemical principles underlying the degradation of PAHs were examined. In addition, this review summarizes the information known about the biochemical processes that determine the fate of the individual components of PAH mixtures in polluted ecosystems. A deeper understanding of the microorganism-mediated mechanisms of catalysis of PAHs will facilitate the development of new methods to enhance the bioremediation of PAH-contaminated sites.

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“…The rise in actinobacterial transcript numbers in phenanthrene-amended soil largely represented an increase in the suborders Corynebacterineae and Micrococcineae, particularly of members of the genera Mycobacterium and Arthrobacter. Both Mycobacterium and Arthrobacter species have being implicated in PAH degradation, and a number of isolates of these organisms capable of PAH metabolism have been described in the literature (Guerin and Jones, 1988;Grifoll et al, 1992;Boldrin et al, 1993;Casellas et al, 1997;Samanta et al, 1999;Moody et al, 2001;van Herwijnen et al, 2003;Seo et al, 2006), most of which are capable of phenanthrene metabolism (Mallick et al, 2011). Mycobacterium spp.…”

Section: Figmentioning

confidence: 99%

Comparative metatranscriptomics reveals widespread community responses during phenanthrene degradation in soil

Menezes

Clipson

Doyle

2012

Environmental Microbiology

137

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Soil microbial community response to phenanthrene was evaluated by metatranscriptomics. A marked increase in transcripts involved in aromatic compound metabolism, respiration and stress responses, and concurrent decreases in virulence, carbohydrate, DNA metabolism and phosphorus metabolism transcripts was revealed. Phenanthrene addition led to a 1.8-fold to 33-fold increase in the abundance of dioxygenase, stress response and detoxification transcripts, whereas those of general metabolism were little affected. Heavy metal P-type ATPases and thioredoxin transcripts were more abundant in the phenanthrene-amended soil, and this is the first time these proteins have been associated with polycyclic aromatic hydrocarbon (PAH) stress in microorganisms. Annotation with custom databases constructed with bacterial or fungal PAH metabolism protein sequences showed that increases in PAH-degradatory gene expression occurred for all gene groups investigated. Taxonomic determination of mRNA transcripts showed widespread changes in the bacteria, archaea and fungi, and the actinobacteria were responsible for most of the de novo expression of transcripts associated with dioxygenases, stress response and detoxification genes. This is the first report of an experimental metatranscriptomic study detailing microbial community responses to a pollutant in soil, and offers information on novel in situ effects of PAHs on soil microbes that can be explored further.

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Isolation and characterization of a fluorene-degrading bacterium: identification of ring oxidation and ring fission products

Cited by 90 publications

References 34 publications

3,4‐Dihydrocoumarin hydrolase with haloperoxidase activity from Acinetobacter calcoaceticus F46

3,4‐Dihydrocoumarin hydrolase with haloperoxidase activity from Acinetobacter calcoaceticus F46

Microbial biodegradation of polyaromatic hydrocarbons

Comparative metatranscriptomics reveals widespread community responses during phenanthrene degradation in soil

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