Isolation and characterization of a novel mesophilic, fresh-water methanogen from river sediment Methanoculleus oldenburgensis sp. nov.

Blotevogel, Karl-Heinz; Gahl-Janßen, Renate; Jannsen, Sigrid; Fischer, Ulrich; Pilz, Frank; Auling, Georg; Macario, Alberto J. L.; Tindall, Brian J.

doi:10.1007/bf00245335

Cited by 27 publications

(7 citation statements)

References 28 publications

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“…3c), consisting of a cell wall and a cytoplasmic membrane. The cells were easily solubilized with 1% sodium dodecylsulfate (SDS) indicating that the cell envelope consisted of a protein S layer [35]. The cells were generally very sensitive to physical or chemical treatment.…”

Section: Resultsmentioning

confidence: 99%

Mesophilic syntrophic acetate oxidation during methane formation in biogas reactors

Schnürer

Zellner

Svensson

1999

FEMS Microbiology Ecology

122

116

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The reaction pathway for the formation of methane from acetate was investigated in sludge from 13 different biogas reactors. By following the conversion of [2‐14C]acetate and [14C]bicarbonate it was shown that methane formation by syntrophic acetate oxidation was the dominating mechanism for acetotrophic methanogenesis in sludge containing high levels of salts, mainly ammonium, and volatile fatty acids. In one biogas reactor the degree of syntrophic acetate oxidation increased as the concentrations of salts and volatile fatty acids increased. Statistical evaluation with principal component analysis showed a close correlation between the degree of syntrophic acetate oxidation and the concentrations of ammonium and potassium. The acetate degradation rate was lower (10–800 times) in biogas reactors in which methane was formed through syntrophic acetate oxidation than in biogas reactors where acetate was directly cleaved to methane and carbon dioxide. Microscopic observations revealed a predominance of one type of methanogen in the reactors with syntrophic acetate oxidation. Isolation and characterization, including substrate spectrum, mol% G+C, polyamine and 16S rRNA analysis, showed that the strains were hydrogenotrophic methanogens belonging to the genus Methanoculleus.

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Section: Resultsmentioning

confidence: 99%

Mesophilic syntrophic acetate oxidation during methane formation in biogas reactors

Schnürer

Zellner

Svensson

1999

FEMS Microbiology Ecology

122

116

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“…Each of them showed some specific characteristics in methanogenic metabolism. Methanoculleus bourgensis was reported to use H 2 –CO 2 or formate as a substrate for growth and methanogenesis, and is a hydrogenotrophic methanogen (Blotevogel et al. 1992).…”

Section: Discussionmentioning

confidence: 99%

The structure of the bacterial and archaeal community in a biogas digester as revealed by denaturing gradient gel electrophoresis and 16S rDNA sequencing analysis

Liu

Wang

Zhang

et al. 2009

Journal of Applied Microbiology

134

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“…The genus Methanoculleus is comprised of eight species (Romesser et al, 1979;Rivard & Smith, 1982;Corder et al, 1983;Ollivier et al, 1986;Maestrojuan et al, 1990;Blotevogel et al, 1991;Zellner et al, 1998;Dianou et al, 2001;Mikucki et al, 2003). However, the phenotypic and phylogenetic characteristics of M. bourgensis MS2 T , M. olentangyi RC/ER T and M. oldenburgensis CB-1 T suggest that they are subjective synonyms (Asakawa & Nagaoka, 2003).…”

Section: Discussionmentioning

confidence: 99%

“…The genus Methanoculleus, described by Maestrojuan et al (1990), is composed of eight species: Methanoculleus bourgensis, Methanoculleus thermophilus, Methanoculleus olentangyi, Methanoculleus marisnigri, Methanoculleus palmolei, Methanoculleus oldenburgensis, Methanoculleus chikugoensis and Methanoculleus submarinus (Romesser et al, 1979;Rivard & Smith, 1982;Corder et al, 1983;Ollivier et al, 1986;Maestrojuan et al, 1990;Blotevogel et al, 1991;Zellner et al, 1998;Dianou et al, 2001;Mikucki et al, 2003). All species can grow on H 2 /CO 2 or sodium formate as substrates for methanogenesis; only M. chikugoensis and M. palmolei use secondary alcohols.…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization ofMethanoculleus receptaculisp. nov. from Shengli oil field, China

Cheng

Qiu

et al. 2008

FEMS Microbiology Letters

102

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Three strictly anaerobic, thermophilic methanogens (ZC-2T, ZC-3 and ZC-6) were isolated from Shengli oil field, China. The 16S rRNA gene sequences of the three strains were nearly identical, possessing > 99.8% sequence similarity. They also possessed high sequence similarity, 97.4%, to Methanoculleus palmolei strain INSLUZ(T) (97.4% and 97.5%, respectively), indicating that they represented a novel species within the genus Methanoculleus. Cells of strain ZC-2T were nonmotile cocci, 0.8-1.7 microm in diameter, and always occurred singly or in pairs. The three strains used H2/CO2 or sodium formate as substrates for methanogenesis but not sodium acetate, trimethylamine, monomethylamine, ethanol, dimethyl sulfide, isopropanol, isobutanol, butan-2-ol or H2/CO. Optimum growth of strain ZC-2T occurred in the presence of 0.2 M NaCl, pH 7.5-7.8 and temperature 50-55 degrees C with a specific growth rate of 0.084 h(-1). The mol% G+C content of the genomic DNA was 55.2 mol%. Based on these phenotypic and phylogenetic characteristics, strains ZC-2T, ZC-3 and ZC-6 are proposed to represent a novel species in the genus Methanoculleus and named Methanoculleus receptaculi sp. nov. The type strain is ZC-2T (CGMCC 1.5087T=DSM 18860T).

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Isolation and characterization of a novel mesophilic, fresh-water methanogen from river sediment Methanoculleus oldenburgensis sp. nov.

Cited by 27 publications

References 28 publications

Mesophilic syntrophic acetate oxidation during methane formation in biogas reactors

Mesophilic syntrophic acetate oxidation during methane formation in biogas reactors

The structure of the bacterial and archaeal community in a biogas digester as revealed by denaturing gradient gel electrophoresis and 16S rDNA sequencing analysis

Isolation and characterization ofMethanoculleus receptaculisp. nov. from Shengli oil field, China

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