Isolation and characterization of a rhabdovirus from snakehead fish(<i>Ophicephalus striatus</i>)

WeiWei, Zeng; Wang, Qing; Wang, Yingying; Liu, Chun; Tan, Aiping; Shi, Chao; Shu-qin, WU

doi:10.3724/sp.j.1231.2013.38588

Cited by 7 publications

(4 citation statements)

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“…In contrast to most marine or freshwater rhabdovirus, MSRV displays age specificity in which it can induce serious disease solely in juvenile fish. Previous research has reported that the typical clinical symptoms (such as severe internal hemorrhaging) of rhabdovirus-infected fish, like C. maculata and Ophicephalus striatus, are observed (Luo et al, 2013;Zeng et al, 2013). In this research, M. salmoides fingerling fish with similar internal hemorrhage in gills and liver were sampled.…”

Section: Discussionmentioning

confidence: 90%

Isolation and characterization of a novel strain (YH01) of Micropterus salmoides rhabdovirus and expression of its glycoprotein by the baculovirus expression system

Lyu

Yuan

Zhang

et al. 2019

J. Zhejiang Univ. Sci. B

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As one of the most important aquatic fish, Micropterus salmoides suffers lethal and epidemic disease caused by rhabdovirus at the juvenile stage. In this study, a new strain of M. salmoides rhabdovirus (MSRV) was isolated from Yuhang, Zhejiang Province, China, and named MSRV-YH01. The virus infected the grass carp ovary (GCO) cell line and displayed virion particles with atypical bullet shape, 300-500 nm in length and 100-200 nm in diameter under transmission electron microscopy. The complete genome sequence of this isolate was determined to include 11 526 nucleotides and to encode five classical structural proteins. The construction of the phylogenetic tree indicated that this new isolate is clustered into the Vesiculovirus genus and most closely related to the Siniperca chuatsi rhabdovirus. To explore the potential for a vaccine against MSRV, a glycoprotein (1-458 amino acid residues) of MSRV-YH01 was successfully amplified and cloned into the plasmid pFastBac1. The high-purity recombinant bacmid-glycoprotein was obtained from DH10Bac through screening and identification. Based on polymerase chain reaction (PCR), western blot, and immunofluorescence assay, recombinant virus, including the MSRV-YH01 glycoprotein gene, was produced by transfection of SF9 cells using the pFastBac1-gE2, and then repeatedly amplified to express the glycoprotein protein. We anticipate that this recombinant bacmid system could be used to challenge the silkworm and develop a corresponding oral vaccine for fish.

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Section: Discussionmentioning

confidence: 90%

Isolation and characterization of a novel strain (YH01) of Micropterus salmoides rhabdovirus and expression of its glycoprotein by the baculovirus expression system

Lyu

Yuan

Zhang

et al. 2019

J. Zhejiang Univ. Sci. B

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“…Six cell lines derived from cyprinid fish, EPC, FHM, Koi proboscis cells, grass carp swim bladder (GSB) cells, Ctenopharyngodon idella kidney (CIK) cells and Ctenopharyngodon idella brain (CIB) cells, have been tested for their susceptibility to SHVV (Zeng et al, 2013). The results showed that Koi proboscis cells were not susceptible to the virus.…”

Section: Discussionmentioning

confidence: 99%

Breaking the host range: mandarin fish is susceptible to a vesiculovirus derived from snakehead fish

Liu

Wen

et al. 2015

Journal of General Virology

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Members of the genus Vesiculovirus, which belongs to the family Rhabdoviridae, can cause great economic loss in fish culture. In the present report, a vesiculovirus [named snakehead fish vesiculovirus (SHVV)] was isolated from diseased hybrid snakehead fish. SHVV shared 94 % nucleotide sequence identity at the genomic level with Siniperca chuatsi rhabdovirus (SCRV), which infects mandarin fish (S. chuatsi). We showed that SHVV was able to replicate and proliferate well in SSN-1 cells, which originate from striped snakehead fish (Channa striatus). Furthermore, mandarin fish was susceptible to SHVV by bath exposure, as well as by intraperitoneal injection. The infected fish showed typical clinical signs of rhabdovirus infection, including haemorrhage and oedema. Histopathological analysis revealed that extensive inflammation and necrosis were observed in the spleen, kidney, liver, heart and brain of the moribund mandarin fish. These results will shed new light on the epidemic of vesiculovirus infections among fish.

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“…Each sampled diseased channel catfish was externally disinfected with 75% alcohol and dissected. Before conducting a careful examination for parasites and viruses as described by Yang & Yang (2013) and Zeng et al (2013), 0.05g affected intestine of each diseased fish was removed and streaked onto nutrient agar (NA) plates (Sinopharm Chemical Reagent Co., Ltd.). After incubation for 24h at 28 0 C, the dominant uniform isolates were purified by streaking and re-streaking onto NA plates.…”

Section: Methodsmentioning

confidence: 99%

Enterobacter aerogenes: an Emerging Pathogen for Enteritis in Farmed Channel Catfish Ictalurus punctatus

Yang¹,

Ai²,

Lu³

et al. 2017

Israeli Journal of Aquaculture - Bamidgeh

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Enteritis has caused significant economic losses in farmed channel catfish Ictalurus punctatus. Yet information is limited on Enterobacter aerogenes as a potential causal agent for enteritis in channel catfish. In this study, a virulent strain, temporarily named HT2, was isolated from diseased channel catfish suffering from enteritis, identified phenotypically and molecularly as E. aerogenes. A phylogenetic tree was constructed to examine isolate HT2 and compare it to other known isolates. In addition, isolate HT2 is apparently susceptible to aminoglycosides and quinolones, drugs for veterinary use in aquaculture as seen when screened against a range of common antibiotics. To the best of our knowledge, this is the first report of E. aerogenes as a pathogen causing enteritis in farmed channel catfish. The IJA appears exclusively as a peer-reviewed on-line open-access journal at http://www.siamb.org.il. To read papers free of charge, please register online at registration form. Sale of IJA papers is strictly forbidden.

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Isolation and characterization of a rhabdovirus from snakehead fish(Ophicephalus striatus)

Cited by 7 publications

References 0 publications

Isolation and characterization of a novel strain (YH01) of Micropterus salmoides rhabdovirus and expression of its glycoprotein by the baculovirus expression system

Isolation and characterization of a novel strain (YH01) of Micropterus salmoides rhabdovirus and expression of its glycoprotein by the baculovirus expression system

Breaking the host range: mandarin fish is susceptible to a vesiculovirus derived from snakehead fish

Enterobacter aerogenes: an Emerging Pathogen for Enteritis in Farmed Channel Catfish Ictalurus punctatus

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