Isolation and characterization of a copalyl diphosphate synthase gene promoter from Salvia miltiorrhiza

Szymczyk, Piotr; Skała, Ewa; Grąbkowska, Renata; Jeleń, Agnieszka; Żebrowska, Marta; Balcerczak, Ewa

doi:10.5586/asbp.3513

Cited by 8 publications

(11 citation statements)

References 69 publications

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“…The lack of these sequences in the seven tested S. mitiorrhiza genes may be related to the observed scarcity of metabolic response to auxin treatment (Table S4). Previous studies suggest that IAA 2.85 μM (0.5 mg L −1 ) treatment does not induce of SmHMGR2 gene expression (Szymczyk et al 2018); this is consistent with the lack of tested auxin-responsive cis-active motifs in SmHMGR2 or its A. thaliana homolog (Table S4) (Fig. 4).…”

Section: Distribution Of Auxin Responsive Cis-active Elements Within Proximal Promoters Of a Thaliana And S Mitiorrhizasupporting

confidence: 87%

“…It is possible that SA and NAA elicitation may influence the tanshinone biosynthesis rate though inhibiting the transcription activity of most ARFs in S. miltiorrhiza (Xu et al 2016). Also, the expression of certain genes, such as SmHMGR2, in S. miltiorrhiza is not induced by auxin IAA (Szymczyk et al 2018).…”

Section: Discussionmentioning

confidence: 99%

“…4). Among these three, the SmCPS and SmHMGR2 genes were verified experimentally by RT-PCR studies in transgenic plants (Szymczyk et al 2016(Szymczyk et al , 2018. Similarly, the S. miltiorrhiza genes SmIDI, SmGGPPS, SmHMGS, SmHDR and SmDXR have also been experimentally-confirmed to respond to SA treatment (Hua et al 2012;Hao et al 2013Hao et al , 2015Jiang et al 2019;Yan et al 2009;Zhang et al 2010Zhang et al , 2016.…”

Section: Distribution Of Sa Responsive Cis-active Elements Within Proximal Promoters Of a Thaliana And S Miltiorrhizamentioning

confidence: 97%

“…The IDP and DMADP moiety is used for the stepwise biosynthesis of a 20-carbon geranylgeranyl diphosphate (GGPP), which then serves as a substrate for copalyl diphosphate synthase 1 and 2, producing (+)-copalyl diphosphate. The (+)-copalyl diphosphate is transformed into miltiradiene, the first committed tanshinone biosynthesis pathway intermediate, by the kaurene-like synthase 1 (Bai et al 2018;Guo and Ma 2019;Szymczyk et al 2016).…”

Section: Introductionmentioning

confidence: 99%

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Elicitation of solid callus cultures of Salvia miltiorrhiza Bunge with salicylic acid and a synthetic auxin (1-naphthaleneacetic acid)

Szymczyk

Szymańska

Kochan

et al. 2021

Plant Cell Tiss Organ Cult

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The presented study analyses the influence of salicylic acid (SA) and the synthetic auxin 1-naphthaleneacetic acid (NAA) on total tanshinone level and on dihydrotanshinone (DHT), cryptotanshinone (CT), tanshinine I (TI) and tanshinone IIA (TIIA) level in Salvia miltiorrhiza callus cultures growing on solid Murashige and Skoog (MS) medium. The influence of SA and NAA was evaluated at 10-day intervals throughout a 80-day treatment period. SA was applied at 0.1, 0.2 and 0.4 mM, and NAA at 2.69, 13.43, 26.85 and 40.28 μM. DHT, CT, TI and TIIA concentrations were measured using HPLC. NAA did not increase the concentration of any tanshinone. SA increased content in a concentration- and time-dependent manner; however, the yields were relatively low, possibly due to the metabolic specificity of S. miltiorrhiza cultivars in Poland. Total tanshinone concentration reached 226.38 ± 37.33 μg g−1 DW after 50 days of 0.4 mM SA elicitation. After 50 days of SA elicitation, the following maximum tanshinone concentrations were observed for 0.4 mM SA: DHT (71.58 ± 12.72 μg g−1 DW), CT (108.54 ± 18.29 μg g−1 DW), TI (29.50 ± 4.13 μg g−1 DW) and TIIA (16.75 ± 2.74 μg g−1 DW). To account for these observed differences in tanshinone biosynthesis, the distribution of SA and auxin responsive cis-active motif in the proximal promoters of the mevalonic acid, methylerythritol-4-phosphate and tanshinone-precursor biosynthesis pathway genes was evaluated in A. thaliana and S. miltiorrhiza. Our findings indicate that the SA-responsive cis-active elements have a much broader distribution than those recognized by auxin-responsive transcription factors.

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Section: Distribution Of Auxin Responsive Cis-active Elements Within Proximal Promoters Of a Thaliana And S Mitiorrhizasupporting

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Section: Distribution Of Sa Responsive Cis-active Elements Within Proximal Promoters Of a Thaliana And S Miltiorrhizamentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

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Elicitation of solid callus cultures of Salvia miltiorrhiza Bunge with salicylic acid and a synthetic auxin (1-naphthaleneacetic acid)

Szymczyk

Szymańska

Kochan

et al. 2021

Plant Cell Tiss Organ Cult

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“…This result indicated that there may be relationships between the gene expression of the CPS promoter and the demethylation effect of 5-Az. Based on the demonstration by Szymczyk et al (2016), many TF binding sites can be observed in the CPS promoter region. We found that 5-Az promoted a demethylation effect on some TF binding sites, and approximately thirty-six different TFs were recognized by PLANTPAN3.0.…”

Section: Discussionmentioning

confidence: 99%

5-Azacytidine Increases Tanshinone Production in Salvia miltiorrhiza Hairy Roots Through Epigenetic Modulation

Yang¹,

Lee²,

Lin³

et al. 2021

Preprint

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Recent studies have indicated strong connections between epigenetic modulation and secondary metabolites in plants. It is vital to understand the roles of epigenetics in the production of secondary metabolites. In this study, the DNA demethylation agent 5-azacytidine (5-Az) was used on the hairy roots of the medicinal crop Salvia miltiorrhiza to investigate its effect on secondary metabolite production, gene expression, and methylation levels in gDNA and promoter regions. Our results showed that the contents of tanshinones in S. miltiorrhiza hairy roots increased by 1.5 to 5 times, and most genes in the MEP pathway showed an upward trend. According to our NGS analysis, the methylation pattern in the copalyl diphosphate synthase (CPS) promoter was altered, and 51 out of 145 cytosines were demethylated during 5-Az treatment. A total of 36 different transcription factors (TFs) were identified in these demethylation sites. Among these TFs, NF-Y and MYB were frequently found in our results. This is the first report to demonstrate a possible mechanism of DNA methylation participating in tanshinone biosynthesis in S. miltiorrhiza hairy roots by modulating the CPS promoter and TFs.

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Identification and functional characterization of the CVOMTs and EOMTs genes promoters from Ocimum basilicum L.

Khakdan

Shirazi

Ranjbar

2022

Plant Cell Tiss Organ Cult

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Methyl chavicol and methyl eugenol are important phenylpropanoid compounds previously puri ed from basil. These compounds are signi cantly enhanced by the water de cit stress-dependent mechanism. Here, for the rst time, pObCVOMT and pObEOMT promoters were extracted by the genome walking method. They were then cloned into the upstream of the β-glucuronidase (GUS) reporter gene to identify the pattern of GUS water de cit stress-speci c expression. Histochemical GUS assays showed in transgenic tobacco lines bearing the GUS gene driven by pObCVOMT and pObEOMT promoters, GUS was strongly expressed under water de cit stress. qRT-PCR analysis of pObCVOMT and pObEOMT transgenic plants con rmed the histochemical assays, indicating that the GUS expression is also signi cantly induced and up-regulated by increasing density of water de cit stress. This indicates these promoters are able to drive inducible expression. The cis-acting elements analysis showed that the pObCVOMT and pObEOMT promoters contained dehydration or water de cit-related transcriptional control elements.

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Isolation and characterization of a copalyl diphosphate synthase gene promoter from Salvia miltiorrhiza

Cited by 8 publications

References 69 publications

Elicitation of solid callus cultures of Salvia miltiorrhiza Bunge with salicylic acid and a synthetic auxin (1-naphthaleneacetic acid)

Elicitation of solid callus cultures of Salvia miltiorrhiza Bunge with salicylic acid and a synthetic auxin (1-naphthaleneacetic acid)

5-Azacytidine Increases Tanshinone Production in Salvia miltiorrhiza Hairy Roots Through Epigenetic Modulation

Identification and functional characterization of the CVOMTs and EOMTs genes promoters from Ocimum basilicum L.

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