Isolation and Characterization of a Human Intestinal Bacterium <i>Eggerthella</i> sp. AUH-JLD49s for the Conversion of (−)-3′-Desmethylarctigenin

Wang, Ye; Yu, Fang; Liu, Mingyue; Zhao, Yi-Kai; Wang, Dongming; Hao, Qing‐Hong; Wang, Xiuling

doi:10.1021/acs.jafc.7b00114

Cited by 5 publications

(3 citation statements)

References 21 publications

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“…LMG11000, Pseudomonas oleovorans, Eggerthella and Burkholderia, but decreased that from Azoarcus. A recent study by Wang et al (2017) reported that the metabolite by Eggerthella showed an inhibitory effect on growth of human colon cancer line. In addition, the relative abundance of Eggerthella was reduced in Rett syndrome subjects as compared to healthy controls (Strati et al 2016).…”

Section: Genusmentioning

confidence: 99%

Diversity of intestinal bacterial lactase gene in antibiotics-induced diarrhea mice treated with Chinese herbs compound Qi Wei Bai Zhu San

Liu

Guo

et al. 2017

3 Biotech

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The current investigation is trying to study the impact of the mixture of Chinese herbs Qi Wei Bai Zhu San (QWBZS) on bacterial lactase gene from antibiotics-induced diarrhea (AAD) mice, as the good curative effect of QWBZS on diarrhea. Mice (6 mice per group) were randomly selected as control, model and treatment groups. To induce diarrhea, mice in both model and treatment groups were intragastrically injected with mixture of gentamycin sulfate and cefradine (23.33 mL kg day) twice per day and continuously for totally 5 days. After the success of establishing diarrhea model, the mice in treatment group were gavaged with QWBZS for 3 days. Intestinal contents in all three groups were then collected and DNA was extracted in aseptic environment for the following sequencing. The results showed that mice from QWBZS treatment group had obviously detectable levels of intestinal bacteria, such as Actinobacteria, Firmicutes and Proteobacteria, which produce phyla lactase specifically. In comparison with other groups, the mice in treatment group had more abundant expression of lactase gene from sp. KKs102, sp. LMG11000, , and . Interestingly, the Shannon index decreased significantly after the treatment with QWBZS ( < 0.01 or < 0.05). 63.1% of lactase genes detected in the mice in treatment group were unclassified, and 32.8% of them were non-homologous to any fragments in the gene bank, which means that most of lactase-producing bacteria are novel. Our results indicate that treatment with QWBZS did not increase the diversity of bacterial lactase gene. Its curative effect on diarrhea may be relevant to its role in facilitating the growth of novel or some key lactase-producing strains.

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Section: Genusmentioning

confidence: 99%

Diversity of intestinal bacterial lactase gene in antibiotics-induced diarrhea mice treated with Chinese herbs compound Qi Wei Bai Zhu San

Liu

Guo

et al. 2017

3 Biotech

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“…Colorectal cancer (CRC) is one of the most frequent cancers, with more than 1.2 million new cases and 500,00 deaths annually around the world, the cornerstones of therapy are surgery, radiotherapy (for patients with rectal cancer), and chemotherapy [ 1 ]. Triptolide, the major active component of Triptergium wilfordii Hook.…”

Section: Introductionmentioning

confidence: 99%

Mechanisms of action of triptolide against colorectal cancer: insights from proteomic and phosphoproteomic analyses

Song

Zhang

et al. 2022

Aging

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Triptolide is a potent anti-inflammatory agent that also possesses anticancer activity, including against colorectal cancer (CRC), one of the most frequent cancers around the world. In order to clarify how triptolide may be effective against CRC, we analyzed the proteome and phosphoproteome of CRC cell line HCT116 after incubation for 48 h with the drug (40 nM) or vehicle. Tandem mass tagging led to the identification of 403 proteins whose levels increased and 559 whose levels decreased in the presence of triptolide. We also identified 3,110 sites in proteins that were phosphorylated at higher levels and 3,161 sites phosphorylated at lower levels in the presence of the drug. Analysis of these differentially expressed and/or phosphorylated proteins showed that they were enriched in pathways involving ribosome biogenesis, PI3K−Akt signaling, MAPK signaling, nucleic acid binding as well as other pathways. Protein–protein interactions were explored using the STRING database, and we identified nine protein modules and 15 hub proteins. Finally, we identified 57 motifs using motif analysis of phosphosites and found 16 motifs were experimentally verified for known protein kinases, while 41 appear to be novel. These findings may help clarify how triptolide works against CRC and may guide the development of novel treatments.

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“…The same group has also reported that Blautia sp. AUH-JLD56 could biotransform arctin or arctigenin into 3′-DMAG (Liu et al, 2013;Wang, Yu, et al, 2017). They have evaluated the DPPH radical-scavenging activity of 3′-DMAG (Wang, Solorzano, et al, 2017), yet its anticancer activity remains unknown.…”

Section: Introductionmentioning

confidence: 99%

3′‐Desmethylarctigenin induces G2/M cell cycle arrest and apoptosis through reactive oxygen species generation in hepatocarcinoma cells

Zhang

Wang

Liu

et al. 2019

Phytotherapy Research

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Previous studies have shown that arctigenin is a promising chemopreventive or therapeutic agent against various cancers. However, less is known about anticancer activity of 3′‐desmethylarctigenin (3′‐DMAG), which is a biotransformed product from arctigenin or arctin. In this study, we compared the anticancer activity of 3′‐DMAG with its parent compound arctigenin and demonstrated that 3′‐DMAG exerted a more potent inhibitory effect on HepG2 cells than arctigenin. Mechanistically, reactive oxygen species generation played an apical role in 3′‐DMAG‐induced G2/M cell cycle arrest and apoptosis in HepG2 cells. Furthermore, the Chk2–Cdc25c–Cdc2–cyclin B1 cascade was found to contribute to the cell cycle arrest, whereas the activation of mitochondrial pathway was involved in the cell apoptosis by 3′‐DMAG. Additionally, a mouse xenograft hepatocellular carcinoma model was used to evaluate the antitumor effect of 3′‐DMAG in vivo, and the results indicated that 3′‐DMAG treatment significantly inhibited tumor growth without apparent toxicity. Taken together, 3′‐DMAG is highly effective against liver cancer both in vitro and in vivo. The findings of the present study suggest that this compound deserves to be further investigated for its potential anticancer activity.

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Isolation and Characterization of a Human Intestinal Bacterium Eggerthella sp. AUH-JLD49s for the Conversion of (−)-3′-Desmethylarctigenin

Cited by 5 publications

References 21 publications

Diversity of intestinal bacterial lactase gene in antibiotics-induced diarrhea mice treated with Chinese herbs compound Qi Wei Bai Zhu San

Diversity of intestinal bacterial lactase gene in antibiotics-induced diarrhea mice treated with Chinese herbs compound Qi Wei Bai Zhu San

Mechanisms of action of triptolide against colorectal cancer: insights from proteomic and phosphoproteomic analyses

3′‐Desmethylarctigenin induces G2/M cell cycle arrest and apoptosis through reactive oxygen species generation in hepatocarcinoma cells

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