Isolation and characterization of a novel phenanthrene (PHE) degrading strain Psuedomonas sp. USTB-RU from petroleum contaminated soil

Masakorala, Kanaji; Yao, Jun; Cai, Minmin; Chandankere, Radhika; Yuan, Haiyan; Chen, Huilun

doi:10.1016/j.jhazmat.2013.10.007

Cited by 51 publications

(28 citation statements)

References 34 publications

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“…Phe-1, with high degradation efficiency was selected by screening bacteria collected from a site highly contaminated by PAHs near a refinery in Qingyuan, Guangdong province. According to the literature [40], soil suspension was prepared by placing 10 g fresh soil in 90 mL Mill-Q water and shaking for 30 min at 180 rpm at 30 °C. Then 1 mL soil suspension was inoculated into 250 mL Erlenmeyer flask containing 99 mL of sterilized MM supplemented with 1 mg of phenanthrene and incubated aerobically for 5 d at 30 °C at 160 rpm.…”

Section: Bacteriummentioning

confidence: 99%

Effects of dissolved organic matter derived from forest leaf litter on biodegradation of phenanthrene in aqueous phase

Cai

Yang

Wang

et al. 2017

Journal of Hazardous Materials

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Dissolved organic matter (DOM) released from forest leaf litter is potentially effective for the degradation of polycyclic aromatic hydrocarbons (PAHs), yet the inherent mechanism remains insufficiently elucidated. In this study, we investigated the effects of DOM derived from Pinus elliottii and Schima superba leaf litter on the degradation of phenanthrene by the phenanthrene degrading bacterium Sphingobium sp. Phe-1. DOM from different origins and at a large range of concentrations enhanced the degradation rate of phenanthrene. DOM derived from P. elliottii leaf litter decomposed for 12 months used at a concentration of 100mg/L yielded the highest degradation rate (16.9% in 36h) and shortened the degradation time from 48h to 24h. Changes in the composition of DOM during degradation as measured by EEMs-FRI showed that proteins and tyrosine in the DOM supplied readily available nutrients that stimulated biological activity of Phe-1, increasing its growth rate and catechol 2,3-dioxygenase activity. Simultaneously, fulvic acid and humic acid in the DOM enhanced phenanthrene bioavailability by increasing the solubility and mass transfer of phenanthrene, enhancing the uptake kinetics of Phe-1, and increasing the bacteria's direct access to DOM-associated phenanthrene. Humic acid was co-metabolized by Phe-1, resulting in further stimulation of phenanthrene degradation.

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Section: Bacteriummentioning

confidence: 99%

Effects of dissolved organic matter derived from forest leaf litter on biodegradation of phenanthrene in aqueous phase

Cai

Yang

Wang

et al. 2017

Journal of Hazardous Materials

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“…strain SMN-1 (Mulla et al 2013) PAH Arthrobacter sp. strain Sphe3 is capable of achieving 90 % removal of PAH (400 mg/l) in 4 days at 30°C in the shaking flask (180 RPM) (Kallimanis et al 2007) Phenanthrene Pseudomonas vesicularis achieves 87 % degradation of phenanthrene (100 mg/l) in 8 days at the temperature of 30°C and pH of 7.0 (Masakorala et al 2013) hydrocarbon-contaminated soils could occur at the temperature as low as 5°C (Paudyn et al 2008). The potential of oil bioremediation in cold terrestrial and marine ecosystems has also been reported (Margesin and Schinner 2001).…”

Section: -Nitrotoluenementioning

confidence: 99%

Diesel degradation in soil catalyzed by the addition of a bioagent

Lin

Lay

Shieh

2015

Int. J. Environ. Sci. Technol.

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The supernatant harvested from a mesophilic, molasses-fed, non-methanogenic bioreactor, which is rich in nitrogen, phosphorous, metals, free amino acids, Sporolactobacillus sp., Prevotella sp., and Clostridium sp., is diluted with tap water and tested as the bioagent to catalyze diesel degradation in soil. Outdoor experiments are performed under the following conditions to assess the effectiveness of the bioagent: diesel doses: 9-15 mg TPH/g soil; bioagent concentrations and dose: 1-5 % at 60 ml/day; soil sample size: 600 g; ambient temperatures: 28-32°C, and relative humidity: 40-82 % (TPH: total petroleum hydrocarbons). Diesel degradation in soil treated with 3 % bioagent, which proceeds at the rates of 1.04-1.55 mg TPH/g soil-day, is completed in about a week with up to 83 % efficiencies. In contrast, diesel degradation in soil sprinkled with water (60 ml/day) proceeds at the rate of 0.3 mg TPH/g soil-day that achieves 15-22 % degradation efficiencies. The addition of 3 % bioagent yields desired soil moisture content (10-15 %), soil pH (6.8-8.2), and nutrition inputs. Both Sporolactobacillus sp. and Prevotella sp. grown on molasses are robust, tolerant high diesel doses, able to utilize hydrophobic hydrocarbons, and readily adaptable to the soil environment. Most notably, prior acclimatization is not required to enable these properties.

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“…Oil sludge also contains aliphatic, aromatic and poliaromatic hydrocarbons (PAHs) that are carcinogenic, mutagenic, and immuno-toxic, and also have the potential to pollute the environment. The Environmental Protection Agency in the United States categorizes 16 types of PAHs as the main environmental polluters (Skupinska et al, 2004;Seo et al, 2012;Masakorala et al, 2013). Naphthalene and phenanthrene are types of PAHs that have two or three rings.…”

Section: Introductionmentioning

confidence: 99%

Growth Response Test on Potential Indigenous Bacteria to Degrade Naphthalene and Phenanthrene on various pH

Hardianti

Lusiana

Sawitri

2019

BSc

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This study aims to understand the growth response of indigenous bacteria originating from oil sludge in Dumai which has the potential as Naphthalene and Fenantren degrading agents based on variations in substrate pH. The isolates used in this study were Isolate A, Isolate E, and Isolate F as a result of isolation from oil sludge in Dumai. Based on the results of the growth response test on various concentration in previous studies, we chose E isolates to be tested for its degradation ability. The bacterial growth response data was obtained based on OD measurements every 24 hours using a spectrophotometer and TPC at 72 hours using Nutrient Agar media. Based on these data, we determined the optimum pH for indigenous bacterial growth potential to degrade naphthalene or phenanthrene. In all variations of pH, bacterial isolate E experienced growth. This shows that bacterium E can use naphthalene and fenantren at normal pH range (pH 5-9).

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Isolation and characterization of a novel phenanthrene (PHE) degrading strain Psuedomonas sp. USTB-RU from petroleum contaminated soil

Cited by 51 publications

References 34 publications

Effects of dissolved organic matter derived from forest leaf litter on biodegradation of phenanthrene in aqueous phase

Effects of dissolved organic matter derived from forest leaf litter on biodegradation of phenanthrene in aqueous phase

Diesel degradation in soil catalyzed by the addition of a bioagent

Growth Response Test on Potential Indigenous Bacteria to Degrade Naphthalene and Phenanthrene on various pH

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