Isolation and characterization of a major allergen from the fish parasite Anisakis simplex

Moneo, Ignacio; Caballero, María Luisa; Ortega, Eva; Alonso, María José

doi:10.1067/mai.2000.106732

Cited by 137 publications

(124 citation statements)

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“…The protein content of the supernatant was 2 mg/ml, as measured by the method of Bradford (5) with bovine serum albumin as a standard. A. simplex major allergen Ani s 1 was isolated from larvae and purified from the crude parasite extract by ethanol precipitation and reversed-phase high-performance liquid chromatography according to the method described in a previous work (14).…”

Section: Methodsmentioning

confidence: 99%

“…In an attempt to improve the diagnosis, we purified and characterized a major immunoglobulin E (IgE)-binding protein from the parasite. This major parasite allergen, named Ani s 1, is a protein from the excretory gland that shows no homology to a previously described protein (14). Recently, we have demonstrated that the detection of IgE specifically directed against Ani s 1 has a high accuracy in the diagnosis of anisakidosis (sensitivity, 86%; specificity, 90%) (6).…”

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confidence: 99%

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High Prevalence of Seropositivity to a Major Allergen of Anisakis simplex , Ani s 1, in Dyspeptic Patients

Toro

Caballero

Baquero

et al. 2004

Clin Vaccine Immunol

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Finding evidence of anisakidosis requires invasive methods. We have developed a serological assay based on the detection of an immunoglobulin E (IgE) specifically directed against Ani s 1 protein, a major parasite allergen of Anisakis simplex, which has shown a high level of accuracy in the diagnosis of anisakidosis. We used this tool to determine the prevalence of anti-Ani s 1 IgE in dyspeptic patients and to investigate if its seropositivity could be related to epidemiological factors other than raw fish consumption. A total of 174 dyspeptic patients who submitted to upper digestive tract endoscopy were studied. Specific IgE against Ani s 1 was determined by immunoblotting. Quantitative information on smoking, alcohol consumption, and fish consumption as well as a history of gastric surgery was recorded. Twenty-four (13.8%) patients were seropositive for Ani s 1 protein. The seroprevalence of anti-Ani s 1 IgE increased with age in patients who were less than 62 years old (P ‫؍‬ 0.047). Seropositivity to Ani s 1 was associated with the consumption of fish in vinegar (P < 0.001), raw fish (P ‫؍‬ 0.001), and smoked fish (P ‫؍‬ 0.007). There was no relationship between anti-Ani s 1 IgE seropositivity and the number of cigarettes smoked (P ‫؍‬ 0.098) or alcohol intake (P ‫؍‬ 0.179). Five patients had undergone previous gastric surgery, and three of those patients were seropositive for Ani s 1 (P ‫؍‬ 0.019). In multivariate analysis, the consumption of fish in vinegar (P ‫؍‬ 0.006), raw fish (P ‫؍‬ 0.017), and smoked fish (P ‫؍‬ 0.002) and a history of gastric surgery (P ‫؍‬ 0.005) were independent factors associated with anti-Ani s 1 IgE detection. In conclusion, at present, anisakidosis might frequently be underdiagnosed, and it might have a clinical role in patients with upper dyspeptic symptoms. Uncooked-fish ingestion and previous gastric surgery were associated with seropositivity for Ani s 1 protein.

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Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

High Prevalence of Seropositivity to a Major Allergen of Anisakis simplex , Ani s 1, in Dyspeptic Patients

Toro

Caballero

Baquero

et al. 2004

Clin Vaccine Immunol

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“…It is not yet known to which family of proteins it belongs because, although it showed certain similarities in amino acid sequence (30 to 40% identity) to Kunitz-type serine protease inhibitors, it lacks appropriate inhibitory activity (247). Ani s 1 (described by Moneo et al and Shimakura et al) is a major allergen (47,187,230,247) that should not be confused with another 21-kDa minor allergen (recognized by only 20% of allergic patients), the encoding cDNA of which was cloned, and this protein was also named Ani s 1 at the same time (18) and is a protein of different structure (belonging to the nematode troponin family).…”

Section: Anisakis Allergensmentioning

confidence: 99%

Anisakis simplex: from Obscure Infectious Worm to Inducer of Immune Hypersensitivity

2008

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SUMMARY Infection of humans with the nematode worm parasite Anisakis simplex was first described in the 1960s in association with the consumption of raw or undercooked fish. During the 1990s it was realized that even the ingestion of dead worms in food fish can cause severe hypersensitivity reactions, that these may be more prevalent than infection itself, and that this outcome could be associated with food preparations previously considered safe. Not only may allergic symptoms arise from infection by the parasites (“gastroallergic anisakiasis”), but true anaphylactic reactions can also occur following exposure to allergens from dead worms by food-borne, airborne, or skin contact routes. This review discusses A. simplex pathogenesis in humans, covering immune hypersensitivity reactions both in the context of a living infection and in terms of exposure to its allergens by other routes. Over the last 20 years, several studies have concentrated on A. simplex antigen characterization and innate as well as adaptive immune response to this parasite. Molecular characterization of Anisakis allergens and isolation of their encoding cDNAs is now an active field of research that should provide improved diagnostic tools in addition to tools with which to enhance our understanding of pathogenesis and controversial aspects of A. simplex allergy. We also discuss the potential relevance of parasite products such as allergens, proteinases, and proteinase inhibitors and the activation of basophils, eosinophils, and mast cells in the induction of A. simplex-related immune hypersensitivity states induced by exposure to the parasite, dead or alive.

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“…The presence of larval stages of Anisakid nematodes in fish is a problem for commercial fishing industries due to the potential risk of transmission of zoonotic diseases such as anisakiasis (6)(7)(8), and foodborne allergenicity (9,10). Currently, fish diseases, in particular those caused by parasites, constitute one of the most important problems and challenges confronting fish farming (11).…”

Section: Introductionmentioning

confidence: 99%

Parasites and hepatic histopathological lesions in lisa (Mugil incilis) from Totumo mash, North of Colombia

Olivero

2013

Rev MVZ Córdoba

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Objective. To assess the levels of parasitism by nematodes and trematodes, as well as the hepatic histopathological alterations present in Mugil incilis (Lisa) from Totumo marsh, North of Colombia. Materials and methods. Between July 2004 and June 2005, 500 fish were collected at Totumo Marsh (75°16'W and 10°44´N), North of Colombia. Morphometric and parasitic parameters were determined for each specimen, and the hepatic histopathological status of the liver was assessed by analyzing liver slides stained with hematoxylin and eosin. Results. Nematode larvae isolated from Mugil incilis corresponded to Contracaecum spp. Parasite prevalence was 60.49%. Parasitic mean abundance and mean intensity were 4.8±1.05 and 7.02±1.49, nematodes per fish, respectively. The correlation between nematode mean abundance and fish length was significant and positive (r=0.525, p<0.0001), but negative for condition factor (r=-0.109, p=0.014). Hepatic histopathological analysis revealed the presence of encapsulated trematode larvae as the main finding. However, the presence of inflammation, granulomas, steatosis and necrosis, were also registered as secondary alterations. Conclusions. Lisas collected at Totumo Marsh are parasitized with nematodes and trematodes. These fish have different histopathological lesions in the liver tissue, being the most important the presence of trematode encapsulated cyst that generate inflammatory reactions, and negatively correlate with morphometric markers of fish health.Key words: Anisakiasis, fishes, nematode, trematode, zoonoses (Source: CAB). RESUMENObjetivo. Evaluar los niveles de parasitismo por nemátodos y tremátodos, así como las alteraciones histopatológicas hepáticas presentes en Mugil incilis (Lisa) de la Ciénaga del Totumo, Norte de Colombia. Materiales y métodos. Entre Julio de 2004 y Junio de 2005, 500 peces fueron recolectados en la ciénaga del Totumo (75°16'W y 10°44´N), norte de Colombia. Índices morfométricos y parasitarios fueron determinados para cada espécimen, y el estatus histopatológico hepático fue evaluado analizando cortes de hígado teñidos con hematoxilina y eosina. Resultados. Los nemátodos aisladosRev.MVZ Córdoba 18(1):3288-3294, 2013. ORIGINAL 3289de Mugil incilis correspondieron a Contracaecum spp. La prevalencia del parásito fue 60.49%. La abundancia media y la intensidad media parasitaria fueron 4.8±1.05 y 7.02±1.49, larvas por pez, respectivamente. La correlación entre la abundancia media de nemátodos y la longitud de las lisas fue significativa y positiva (r=0.525, p<0.0001), pero negativa para el factor de condición (r=-0.109, p=0.014). El análisis de la histopatología hepática reveló la presencia de larvas encapsuladas de tremátodos, como el hallazgo principal. Sin embargo, la presencia de inflamación, granulomas, esteatosis y necrosis, también fueron registradas como alteraciones secundarias. Conclusiones. Las Lisas colectadas en la ciénaga del Totumo están parasitadas con nemátodos y tremátodos. Estos peces poseen diversas lesiones histopatológicas en el te...

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Isolation and characterization of a major allergen from the fish parasite Anisakis simplex

Cited by 137 publications

References 28 publications

High Prevalence of Seropositivity to a Major Allergen of Anisakis simplex , Ani s 1, in Dyspeptic Patients

High Prevalence of Seropositivity to a Major Allergen of Anisakis simplex , Ani s 1, in Dyspeptic Patients

Anisakis simplex: from Obscure Infectious Worm to Inducer of Immune Hypersensitivity

Parasites and hepatic histopathological lesions in lisa (Mugil incilis) from Totumo mash, North of Colombia

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