Isolation and characterization of a novel podovirus which infects burkholderia pseudomallei

Gatedee, Jiraporn; Kritsiriwuthinan, Kanyanan; Galyov, Edouard E.; Shan, Jinyu; Dubinina, E. E.; Intarak, Narin; Clokie, Martha R. J.; Korbsrisate, Sunee

doi:10.1186/1743-422x-8-366

Cited by 24 publications

(44 citation statements)

References 17 publications

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“…At 77.8%, φX216 has one of the broadest strain infectivity ranges reported for a B . pseudomallei phage, comparing favorably with the Thai soil phages ST2 (78%, 49/63) and ST96 (67%, 42/63) [13,14]. φX216 plaques were 1–2 mm in diameter and mostly-clear on the majority of B .…”

Section: Resultsmentioning

confidence: 99%

φX216, a P2-like bacteriophage with broad Burkholderia pseudomallei and B. malleistrain infectivity

et al. 2012

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BackgroundBurkholderia pseudomallei and B. mallei are closely related Category B Select Agents of bioterrorism and the causative agents of the diseases melioidosis and glanders, respectively. Rapid phage-based diagnostic tools would greatly benefit early recognition and treatment of these diseases. There is extensive strain-to-strain variation in B. pseudomallei genome content due in part to the presence or absence of integrated prophages. Several phages have previously been isolated from B. pseudomallei lysogens, for example φK96243, φ1026b and φ52237.ResultsWe have isolated a P2-like bacteriophage, φX216, which infects 78% of all B. pseudomallei strains tested. φX216 also infects B. mallei, but not other Burkholderia species, including the closely related B. thailandensis and B. oklahomensis. The nature of the φX216 host receptor remains unclear but evidence indicates that in B. mallei φX216 uses lipopolysaccharide O-antigen but a different receptor in B. pseudomallei. The 37,637 bp genome of φX216 encodes 47 predicted open reading frames and shares 99.8% pairwise identity and an identical strain host range with bacteriophage φ52237. Closely related P2-like prophages appear to be widely distributed among B. pseudomallei strains but both φX216 and φ52237 readily infect prophage carrying strains.ConclusionsThe broad strain infectivity and high specificity for B. pseudomallei and B. mallei indicate that φX216 will provide a good platform for the development of phage-based diagnostics for these bacteria.

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Section: Resultsmentioning

confidence: 99%

φX216, a P2-like bacteriophage with broad Burkholderia pseudomallei and B. malleistrain infectivity

et al. 2012

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“…Afterward, each sample was diluted 100-fold, and the number of free phages was determined by plaque formation. Adsorption rate was calculated using pseudo first-order kinetics (Hyman and Abedon 2009;Lin et al 2010;Gatedee et al 2011). One-step growth curves were carried out according to Hyman and Abedon (2009) with a 3-min adsorption at 10 7 cfu/ml and multiplicity of infection of 0.1, followed by 2,500-fold dilution to impede second-generation infection and titering at 5-min intervals.…”

Section: Biological Propertiesmentioning

confidence: 99%

Characterization of lytic Pseudomonas aeruginosa bacteriophages via biological properties and genomic sequences

Karumidze

Thomas

Kvatadze

et al. 2012

Appl Microbiol Biotechnol

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Pseudomonas aeruginosa is an important cause of infections, especially in patients with immunodeficiency or diabetes. Antibiotics are effective in preventing morbidity and mortality from Pseudomonas infection, but because of spreading multidrug-resistant bacterial strains, bacteriophages are being explored as an alternative therapy. Two newly purified broad host range Pseudomonas phages, named vB_Pae-Kakheti25 and vB_Pae-TbilisiM32, were characterized as candidates for use in phage therapy. Morphology, host range, growth properties, thermal stability, serology, genomic sequence, and virion composition are reported. When phages are used as bactericides, they are used in mixtures to overcome the development of resistance in the targeted bacterial population. These two phages are representative of diverse siphoviral and podoviral phage families, respectively, and hence have unrelated mechanisms of infection and no cross-antigenicity. Composing bactericidal phage mixtures with members of different phage families may decrease the incidence of developing resistance through a common mechanism.

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“…To cover any remaining subset of resistant strains, one or more additional phages that infect these organisms must be isolated and characterized. B. pseudomallei phages are readily found in soil samples from endemic regions 30 , 31 …”

Section: Resultsmentioning

confidence: 99%

RapidBurkholderia pseudomalleiidentification and antibiotic resistance determination by bacteriophage amplification and MALDI-TOF MS

et al. 2014

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Phage amplification detected by MALDI-TOF MS was investigated for rapid and simultaneous Burkholderia pseudomallei identification and ceftazidime resistance determination. B. pseudomallei ceftazidime susceptible and resistant ΔpurM mutant strains Bp82 and Bp82.3 were infected with broadly targeting B. pseudomallei phage ϕX216 and production of the m/z 37.6 kDa phage capsid protein observed by MALDI-TOF MS over the course of 3 h infections. This allowed for repoducible phage-based bacterial ID within 2 h of the onset of infection. MALDI-TOF MS-measured time to detection correlated with in silico modeling, which predicted an approximate 2 h detection time. Ceftazidime susceptible strain Bp82, while detectable in the absence of the drug, owing to the reliance of phage amplification on a viable host, was not detectable when 10 μg/mL ceftazidime was added at the onset of infection. In contrast, resistant strain Bp82.3 was detected in the same 2 h timeframe both with and without the addition of ceftazidime.

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Isolation and characterization of a novel podovirus which infects burkholderia pseudomallei

Cited by 24 publications

References 17 publications

φX216, a P2-like bacteriophage with broad Burkholderia pseudomallei and B. malleistrain infectivity

φX216, a P2-like bacteriophage with broad Burkholderia pseudomallei and B. malleistrain infectivity

Characterization of lytic Pseudomonas aeruginosa bacteriophages via biological properties and genomic sequences

RapidBurkholderia pseudomalleiidentification and antibiotic resistance determination by bacteriophage amplification and MALDI-TOF MS

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