Isolation and characterization of aLysinibacillusstrain B1-CDA showing potential for bioremediation of arsenics from contaminated water

Abstract

The main objective of this study was to identify and isolate arsenic resistant bacteria that can be used for removing arsenic from the contaminated environment. Here we report a soil borne bacterium, B1-CDA that can serve this purpose. B1-CDA was isolated from the soil of a cultivated land in Chuadanga district located in the southwest region of Bangladesh. The morphological, biochemical and 16S rRNA analysis suggested that the isolate belongs to Lysinibacillus sphaericus. The minimum inhibitory concentration (MIC) value of the isolate is 500 mM (As) as arsenate. TOF-SIMS and ICP-MS analysis confirmed intracellular accumulation and removal of arsenics. Arsenic accumulation in cells amounted to 5.0 mg g(-1) of the cells dry biomass and thus reduced the arsenic concentration in the contaminated liquid medium by as much as 50%. These results indicate that B1-CDA has the potential for remediation of arsenic from the contaminated water. We believe the benefits of implementing this bacterium to efficiently reduce arsenic exposure will not only help to remove one aspect of human arsenic poisoning but will also benefit livestock and native animal species. Therefore, the outcome of this research will be highly significant for people in the affected area and also for human populations in other countries that have credible health concerns as a consequence of arsenic-contaminated water.

Arsenic resistance and accumulation by two bacteria isolated from a natural arsenic contaminated site

“…). These results are in a good agreement with the previous reports on arsenic removal and accumulation by microorganisms . Although the underlying mechanisms are not yet clear, bioaccumulation of arsenic into the cell would be a result of higher uptake and lower efflux of arsenic.…”

Arsenic Removal and Biotransformation Potential of Exiguobacterium Isolated From an Arsenic‐Rich Soil of Chhattisgarh, India

“…It is also clear from the study that the strain As‐9 had great potential in removing both forms of arsenic from the aqueous environment. Arsenic removal by bacteria has been previously reported by various researchers , suggesting the use of microbial biomass as an effective method in the treatment of arsenic‐contaminated groundwater.…”

Role of soil associated Exiguobacterium in reducing arsenic toxicity and promoting plant growth in Vigna radiata

“…1). A number of reports are available which have shown the ability of the bacterial species including PGPR in removing arsenic from their surrounding environment [37,38]. This suggest the possible use of bacterial biomass for the treatment of arsenic contaminated water, soil and sediments.…”