1976
DOI: 10.1016/0003-9861(76)90347-7
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Isolation and characterization of a liver plasma membrane fraction enriched in glucagon-sensitive adenylate cyclase

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1977
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Cited by 15 publications
(7 citation statements)
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“…For comparison, purified preparations were made of the two well characterized enzymes which degrade insulin: insulin-glucagon protease from rat skeletal muscle (9,17) and glutathione insulin transhydrogenase from rat liver (18). These enzyme preparations were electrophoresed and assayed under conditions identical to those used for the membrane preparations.…”
Section: Polyacrylamide Gel Electrophoresis Of Solubilized Membrane Pmentioning
confidence: 99%
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“…For comparison, purified preparations were made of the two well characterized enzymes which degrade insulin: insulin-glucagon protease from rat skeletal muscle (9,17) and glutathione insulin transhydrogenase from rat liver (18). These enzyme preparations were electrophoresed and assayed under conditions identical to those used for the membrane preparations.…”
Section: Polyacrylamide Gel Electrophoresis Of Solubilized Membrane Pmentioning
confidence: 99%
“…Three membrane fractions are obtained, at sucrose densities of 1.23, 1.16, and 1.03. The peak 2 (density, 1.16) membrane fraction possesses the adenylate cyclase activity most responsive to hormonal stimulation (9). This fraction will be referred to hereafter as the purified membrane preparation.…”
Section: Membrane Preparationmentioning
confidence: 99%
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“…Purified rat liver plasma membranes were isolated as described earlier (3). Adenylate cyclase was assayed as described previously (4).…”
Section: Methodsmentioning
confidence: 99%
“…In liver, as well as in most other tissues examined, adenylyl cyclase is associated almost exclusively with the particulate fraction and is considered to be localized mainly in plasma membranes (79,145,149,194). Adenylyl cyclase is situated on the inner surface of plasma membranes (145,161) and is referred to as the catalytic component; other components of the adenylyl cyclase system include: the regulatory (discriminatory or receptor) component, located on the outer surface of the plasma membrane, and the coupling (transducer) component within the membrane, interposed between the regulatory and catalytic subunits.…”
Section: Metabolism Of Cyclic Nucleotides Synthesis Adenylyl Cyclase mentioning
confidence: 99%