Isolation and characterization of a cDNA clone from Lolium temulentum L. encoding for a sucrose hydrolytic enzyme which shows alkaline/neutral invertase activity

Gallagher, Joe; Pollock, Christopher J.

doi:10.1093/jxb/49.322.789

Cited by 41 publications

(37 citation statements)

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“…NI regulates sucrose metabolism in many plants, including Saccharum officinarum, Beta vulgaris, and Citrus reticulata (Hubbard et al, 1991), and plays an important role in monosaccharide-dependent biosynthesis (Nonis et al, 2007). In order to investigate the role of NI in polysaccharide-synthesis regulation, several NI cDNAs have been cloned from Solanum lycopersicum, Hevea brasiliensis, Saccharum officinarum, and Oryza sativa (Gallagher and Pollock, 1998;Murayama and Handa, 2007;Bocock et al, 2008;Liu et al, 2015), and differential NI expression has been reported in different plant tissues, e.g., in tomato, higher mRNA levels have been detected in the fruit and leaves than in the flowers and stems. In contrast, in Hevea brasiliensis, the highest mRNA levels were detected in the bark and leaves.…”

Section: Introductionmentioning

confidence: 99%

Characterization of the alkaline/neutral invertase gene in Dendrobium officinale and its relationship with polysaccharide accumulation

Gao¹,

Xf²,

Jp³

et al. 2016

Genet. Mol. Res.

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ABSTRACT. Dendrobium officinale is one of the most wellknown traditional Chinese medicines, and polysaccharide is its main active ingredient. Many studies have investigated the synthesis and accumulation mechanisms of polysaccharide, but until recently, little was known about the molecular mechanism of how polysaccharide is synthesized because no related genes have been cloned. In this study, we cloned an alkaline/neutral invertase gene from D. officinale (DoNI) by the rapid amplification of cDNA ends (RACE) method. DoNI was 2231 bp long and contained an open reading frame that predicted a 62.8-kDa polypeptide with 554-amino acid residues. An alkaline/neutral invertase conserved domain was predicted from this deduced amino acid sequence, and DoNI had a similar deduced amino acid sequence to Setaria italica and Oryza brachyantha. We also found that DoNI expression in different tissues was closely related to DoNI activity, and more importantly, polysaccharide level. Our results indicate that DoNI is associated with polysaccharide accumulation in D. officinale.

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Section: Introductionmentioning

confidence: 99%

Characterization of the alkaline/neutral invertase gene in Dendrobium officinale and its relationship with polysaccharide accumulation

Gao¹,

Xf²,

Jp³

et al. 2016

Genet. Mol. Res.

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“…The neutral/alkaline invertases have pH optima at neutral or alkaline pH and reside in the cytoplasm (Copeland, 1990;Ricardo and ap Rees, 1970). At the sequence level, they show little homology with acid invertases and fructosyltransferases (Gallagher and Pollock, 1998). The acid invertases which account for the majority of invertase activity in plants, have an acid pH optimum and can be subdivided into two classes, the cell-wall-bound (insoluble) and the vacuolar (soluble).…”

Section: Introductionmentioning

confidence: 99%

Cloning and characterization of a putative fructosyltransferase and two putative invertase genes from the temperate grass Lolium temulentum L.

Gallagher

Cairns

Pollock

2004

Journal of Experimental Botany

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The invertases of Lolium temulentum have been characterized at the enzyme level. However, studies on the expression of the genes coding for these enzymes have been lacking. To elucidate the role of acid`invertase-like' genes in sucrose metabolism and carbon partitioning in Gramineae further, threè invertase-like' homologous clones were isolated from L. temulentum cDNA expression libraries based on leaf tissue, using maize soluble invertase probes. The effect of developmental stage and alterations in carbohydrate status on the expression and tissue distribution of these genes was investigated. The three highly homologous genes (Inv 1:2, Inv 1:4, and FT 2:2) show different patterns of expression and different tissue distribution. Inv 1:2 was predominantly expressed in root tissue. Expression increased during the dark in root and tiller base tissue. Minimal variations in gene expression were observed in leaf tissue following changes in carbohydrate status. Inv 1:4 was predominantly expressed in tiller bases, leaf sheath, and leaf base, with increased expression in tissue samples in the dark period. FT 2:2 was also predominantly expressed in tiller bases, leaf sheath, and leaf base. Higher expression was observed in leaf tissue following increases in carbohydrate content, in a manner that paralleled the regulation and spatial occurrence of fructan in the leaf tissue. Whilst invertases and fructosyltransferases are dif®cult to distinguish at the level of the whole sequence, analysis of 5¢ sequence and speci®c amino acids allows discrimination which correlates with patterns of expression within the tissue. Based on expression patterns and sequence characteristics, it is proposed that Inv 1:2 and Inv 1:4 code for soluble acid invertases, whilst FT 2:2 codes for a fructosyltransferase.

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“…However, there is still a need for further improvement in this trait, and there are a number of genes that could be targeted as candidates, particularly those involved in fructan biosynthesis or breakdown and enzymes involved in sugar metabolism such as invertases (Gallagher et al 2004;Chalmers et al 2005). Here we use a cytosolic neutral/alkaline invertase (Gallagher and Pollock 1998), which has been mapped to a QTL on chromosome 6 for glucose and fructose content in L. perenne . This LpcAI gene encodes an enzyme that hydrolyses sucrose to produce glucose and fructose, the substrates for respiration and biosynthesis of primary and secondary compounds as well as regulation of gene expression by sugars (Gallagher and Pollock 1998;Gallagher et al 2004).…”

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“…Here we use a cytosolic neutral/alkaline invertase (Gallagher and Pollock 1998), which has been mapped to a QTL on chromosome 6 for glucose and fructose content in L. perenne . This LpcAI gene encodes an enzyme that hydrolyses sucrose to produce glucose and fructose, the substrates for respiration and biosynthesis of primary and secondary compounds as well as regulation of gene expression by sugars (Gallagher and Pollock 1998;Gallagher et al 2004). Expression of this neutral/alkaline invertase gene is more or less constant in response to a number of perturbations including variation in sucrose substrate concentration, light, and position in the leaf i.e., age of tissue (Gallagher and Pollock 1998).…”

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Association of Candidate Genes With Flowering Time and Water-Soluble Carbohydrate Content in Lolium perenne (L.)

Skøt

Humphreys

et al. 2007

Genetics

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We describe a candidate gene approach for associating SNPs with variation in flowering time and watersoluble carbohydrate (WSC) content and other quality traits in the temperate forage grass species Lolium perenne. Three analysis methods were used, which took the significant population structure into account. First, a linear mixed model was used enabling a structured association analysis to be incorporated with the nine populations identified in the structure analysis as random variables. Second, a within-population analysis of variance was performed. Third, a tree-scanning method was used, in which haplotype trees were associated with phenotypes on the basis of inferred haplotypes. Analysis of variance within populations identified several associations between WSC, nitrogen (N), and dry matter digestibility with allelic variants within an alkaline invertase candidate gene LpcAI. These associations were only detected in material harvested in one of the two years. By contrast, consistent associations between the L. perenne homolog (LpHD1) of the rice photoperiod control gene HD1 and flowering time were identified. One SNP, in the immediate upstream region of the LpHD1 coding sequence (C-4443-A), was significant in the linear mixed model. Within-population analysis of variance and tree-scanning analysis confirmed and extended this result to the 2118 polymorphisms in some of the populations. The merits of the tree-scanning method are compared to the single SNP analysis. The potential usefulness of the 4443 SNP in marker-assisted selection is currently being evaluated in test crosses of genotypes from this work with turf-grass varieties.

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Isolation and characterization of a cDNA clone from Lolium temulentum L. encoding for a sucrose hydrolytic enzyme which shows alkaline/neutral invertase activity

Cited by 41 publications

References 3 publications

Characterization of the alkaline/neutral invertase gene in Dendrobium officinale and its relationship with polysaccharide accumulation

Characterization of the alkaline/neutral invertase gene in Dendrobium officinale and its relationship with polysaccharide accumulation

Cloning and characterization of a putative fructosyltransferase and two putative invertase genes from the temperate grass Lolium temulentum L.

Association of Candidate Genes With Flowering Time and Water-Soluble Carbohydrate Content in Lolium perenne (L.)

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