Isolation and characterization of a haemolysin from Trichophyton mentagrophytes

Schaufuss, P.; Müller, F.; Valentin‐Weigand, Peter

doi:10.1016/j.vetmic.2007.01.022

Cited by 12 publications

(6 citation statements)

References 28 publications

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“…The haemolytic activity of streptococcal culture supernatants due to the secreted toxin suilysin was determined as described by Schaufuss et al (2007), with some modifications. Briefly, strains 10, 10Δ ccpA and 10Δ sly were grown to the early exponential (P 0 ) growth phase and pelleted by centrifugation.…”

Section: Methodsmentioning

confidence: 99%

Role of glucose and CcpA in capsule expression and virulence of Streptococcus suis

et al. 2011

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Streptococcus suis is one of the most important pathogens in pigs and is also an emerging zoonotic agent. After crossing the epithelial barrier, S. suis causes bacteraemia, resulting in meningitis, endocarditis and bronchopneumonia. Since the host environment seems to be an important regulatory component for virulence, we related expression of virulence determinants of S. suis to glucose availability during growth and to the sugar metabolism regulator catabolite control protein A (CcpA). We found that expression of the virulence-associated genes arcB, representing arcABC operon expression, cps2A, representing capsular locus expression, as well as sly, ofs, sao and epf, differed significantly between exponential and early stationary growth of a highly virulent serotype 2 strain. Deletion of ccpA altered the expression of the surface-associated virulence factors arcB, sao and eno, as well as the two currently proven virulence factors in pigs, ofs and cps2A, in early exponential growth. Global expression analysis using a cDNA expression array revealed 259 differentially expressed genes in early exponential growth, of which 141 were more highly expressed in the CcpA mutant strain 10DccpA and 118 were expressed to a lower extent. Interestingly, among the latter genes, 18 could be related to capsule and cell wall synthesis. Correspondingly, electron microscopy characterization of strain 10DccpA revealed a markedly reduced thickness of the capsule. This phenotype correlated with enhanced binding to porcine plasma proteins and a reduced resistance to killing by porcine neutrophils. Taken together, our data demonstrate that CcpA has a significant effect on the capsule synthesis and virulence properties of S. suis.

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Section: Methodsmentioning

confidence: 99%

Role of glucose and CcpA in capsule expression and virulence of Streptococcus suis

et al. 2011

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“…In another study, Schaufuss et al . isolated and partially purified a soluble haemolysin from the culture supernatant of T . mentagrophytes CBS 388.58.…”

Section: Discussionmentioning

confidence: 99%

Haemolytic and co‐haemolytic (CAMP‐like) activity in dermatophytes

Döğen

Gümral

İlkit

2014

Mycoses

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Dermatophytes are some of the most common fungal pathogens in both humans and animals. These fungi release enzymes (e.g., keratinases) that play roles in their pathogenesis. Little is known about their haemolytic and co-haemolytic (CAMP-like) activities; however, in bacteria, these components play significant roles in pathogenesis. This study characterised these two factors in 45 dermatophyte strains (representing the genera Arthroderma, Epidermophyton, Microsporum and Trichophyton) using Columbia agar (CA) supplemented with 5% bovine, ovine and equine erythrocytes. Haemolysis was best observed on CA supplemented with ovine erythrocytes followed by equine and bovine erythrocytes, while CAMP-like reactions occurred using bovine and ovine but not equine erythrocytes. Haemolytic and CAMP-like activities were best observed using ovine and bovine erythrocytes in CA in 44 and 38 strains at 7 and 3 days respectively. Most dermatophytes recovered from both symptomatic and asymptomatic lesions had haemolytic and CAMP-like activities. We suggest that the haemolytic and CAMP-like activities are not correlated with ecological characteristics, isolation sites or clinical manifestations of dermatophytic fungi. We also believe that this study has the potential to contribute to the existing literature on dermatophytes and dermatophyte pathogenesis.

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“…Therefore, at this point, we propose that haemolysin produced by Malassezia during growth is a very unique and thermo‐stable molecule that is not sensitive to proteases. Recently, several studies have reported other fungal haemolysins with similar properties to Malassezia haemolysins, such as a haemolysin produced by the ascomycetes fungus T. mentagrophytes , which is assumed to be a very small peptide that is stable during protease treatment and high temperatures . However, this haemolysin also has not yet been identified.…”

Section: Discussionmentioning

confidence: 99%

Identification of the haemolytic activity of Malassezia species

Juntachai

Kummasook

Mekaprateep

et al. 2013

Mycoses

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Malassezia species are part of the normal skin flora and are associated with a number of human and animal skin diseases. However, the mechanisms that mediate infection and host-fungal interactions are poorly understood. The haemolytic activity of several microorganisms is considered a factor that contributes to pathogenicity of the organism to humans and animals. This virulence factor was previously identified in several pathogenic fungi that cause systemic mycoses, such as Aspergillus and Candida. In this study, the haemolytic activity of six major Malassezia species, including M. furfur, M. globosa, M. pachydermatis, M. restricta, M. slooffiae and M. sympodialis, was investigated. The haemolytic activity of these species was tested on tryptone soya agar with 5% sheep blood. All the examined Malassezia species produced a halo zone of complete haemolysis. A quantitative analysis of the haemolytic activity was performed by incubating sheep erythrocytes with the extraction from culture of each Malassezia species. Interestingly, M. globosa and M. restricta showed significantly high haemolytic activity compared with the other Malassezia species. In addition, M. globosa also exhibited stable haemolytic activity after treatment at 100 °C and in the presence of some proteases, indicating that this haemolytic factor is different from those of other fungi.

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Isolation and characterization of a haemolysin from Trichophyton mentagrophytes

Cited by 12 publications

References 28 publications

Role of glucose and CcpA in capsule expression and virulence of Streptococcus suis

Role of glucose and CcpA in capsule expression and virulence of Streptococcus suis

Haemolytic and co‐haemolytic (CAMP‐like) activity in dermatophytes

Identification of the haemolytic activity of Malassezia species

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