Isolation and Characterization of a Novel Broad-host-range Bacteriophage Infecting Salmonella enterica subsp. enterica for Biocontrol and Rapid Detection

Choi, In Young; Lee, Ju-Hoon; Kim, Hye-Jin; Park, Mi-Kyung

doi:10.4014/jmb.1711.11017

Cited by 15 publications

(3 citation statements)

References 9 publications

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“…However, antibiotic treatment may no longer be the most effective method for the control of E. coli O157:H7 due to the sharp increase in antibioticresistant infections and strains as a result of the acquisition of genetic capacity [8] and alteration of the microbiome composition of host by antibiotic exposure [9]. Recently, bacteriophage (phage) treatment has emerged as a promising alternative to antibiotics [10,11]. Phages are viruses that have the ability to infect and kill target bacteria [12].…”

Section: Electronic Supplementary Materialsmentioning

confidence: 99%

Isolation and characterization of a novel Escherichia coli O157:H7-specific phage as a biocontrol agent

Lee

Choi

Park

et al. 2020

J Environ Health Sci Engineer

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Purpose Escherichia coli O157:H7 is one of the major foodborne pathogens of global public concern. Bacteriophages (phages) have emerged as a promising alternative to antibiotics for controlling pathogenic bacteria. Here, a lytic E. coli O157:H7-specific phage (KFS-EC) was isolated, identified, and characterized to evaluate its potential as a biocontrol agent for E. coli O157:H7. Methods KFS-EC was isolated from slaughterhouse in Korea. Morphological analysis, genomic analysis and several physiological tests were performed to identify and characterize the KFS-EC. Results A specificity test indicated KFS-EC was strictly specific to E. coli O157:H7 strains among 60 bacterial strains tested. Morphological and phylogenetic analyses confirmed that KFS-EC belongs to the Rb49virus genus, Tevenvirinae subfamily, and the Myoviridae family of phages. KFS-EC genome consists of 164,725 bp and a total of 270 coding sequence features, of which 114 open reading frames (ORFs) were identified as phage functional genes. KFS-EC does not contain genes encoding lysogenic property and pathogenicity, which ensure its safe application. KFS-EC was relatively stable (~1 log decrease) under stressed conditions such as temperatures (20 °C-50 °C), pHs (3-11), organic solvents (ethanol and chloroform), and biocides (0.1% citric acid, 1% citric acid, and 0.1% peracetic acid). KFS-EC was able to inhibit E. coli O157:H7 efficiently at a multiplicity of infection (MOI) of 0.01 for 8 h with greater inhibitory effect and durability and was stable at 4 °C and 22 °C over a 12-week storage period. Conclusions Our results suggest that KFS-EC could be used as a biocontrol agent to E. coli O157:H7.

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Section: Electronic Supplementary Materialsmentioning

confidence: 99%

Isolation and characterization of a novel Escherichia coli O157:H7-specific phage as a biocontrol agent

Lee

Choi

Park

et al. 2020

J Environ Health Sci Engineer

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“…By contrast, the previously characterized C. rodentium phage phiCR1, which targets lipopolysaccharide (LPS) as a receptor, was shown to be highly species-specific infecting only C. rodentium and not closely related Enterobacteriaceae, including C. freundii and E. coli (30). Although, phiCR1 host range supports the general notion that phages are largely species-specific (38,39), several other phages infecting various Enterobacteriaceae (38,40,41) and staphylococci (42) have also been demonstrated to be polyvalent. Polyvalent phages infecting strains across genera raise a question as to their so called "optimal" host and whether a phage's host should be inferred by genomic relationships rather than bacterial strain plaquing in vitro (43).…”

Section: Discussionmentioning

confidence: 86%

Isolation and characterization of bacteriophages that infect Citrobacter rodentium, a model pathogen for investigating human intestinal diseases

Luong

Cedarstrom

et al. 2018

Preprint

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Citrobacter rodentium is a mouse-restricted pathogen that has long been used as an in vivo model for two important human intestinal pathogen enteropathogenic Escherichia coli (EPEC) and enterohaemorrhagic E. coli (EHEC). And yet, in contrast to E. coli, little is known about the bacteriophages (phages) -bacterial viruses -that infect C. rodentium, reflecting in part a need to isolate and compare phages associated with this bacterial species. Here, we isolated two novel virulent phages CrRp3 and CrRp10 that infect C. rodentium and conduct in vitro and comparative genomic studies with other, related phages. We show that CrRp3 should be considered as a new species within the genus Sp6virus in the family Podoviridae, having relatively close relations to E. coli phages K1-E and K1-5 in both gene content and genome organization. By contrast, CrRp10 genome is 98% identical to that of E. coli phage Ime09 and thus should be considered as a new strain of the latter phage. We provide evidence that genomes of CrRp3 and CrRp10 have been shaped by both horizontal genetic exchange and genetic drift. Furthermore, phylogenetic analyses showed that these phages are more closely related to E. coli phages than to those infecting members of the Citrobacter genus, suggesting that CrRp3 and CrRp10 may have evolved from E. coli phages following a host-switch event.

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“…Gencay et al (2019) determined that the combination of phage type and receptor is the main determinant of host spacing in their study of the characterization of Salmonella phages isolated from various sources [27]. Choi et al (2017) reported that two Salmonella phages isolated in their study degraded five Salmonella serotypes [28]. A total of 58 Salmonella phages were isolated from wastewater treatment plants, riverside wastewater, lakeside farm waste, and poultry in the Huang et al ( 2018) study [29].…”

Section: Discussionmentioning

confidence: 99%

In vitro bacteriolytic activity of Salmonella specific novel isolated bacteriophage

Basak EROL,

KASKATEPE

2024

jrp

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Salmonella is an important agent that causes foodborne gastrointestinal infections. Salmonella enterica serovar Enteritidis, serovar Typhimurium, and serovar Infantis are the main pathogenic agents of several enteric infections worldwide. Due to the emergence of multidrug resistant Salmonella strains, there is a need for new administration strategies alternative to antibiotics, such as bacteriophages, for effective control of them. The main objectives of this study were to isolate and characterize Salmonella spp. bacteriophage and compare its activity to commercial phage product and assess the effect of bacteriophages in vitro during 24-h. Because of the study, it was found that the newly isolated and named vB_SiM_12 phage could lyse 100% of the strains tested. Bacterial growth with dilutions of vB_SiM_12 phage (MOI ranging from 0.001 to 10) was measured during 24-h. The optic density values of the samples with added bacteriophage remained lower than the control sample during the 24-h incubation period. The results show that using phages may offer a promising alternative to combat biological control agents against Salmonella infection.

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Isolation and Characterization of a Novel Broad-host-range Bacteriophage Infecting Salmonella enterica subsp. enterica for Biocontrol and Rapid Detection

Cited by 15 publications

References 9 publications

Isolation and characterization of a novel Escherichia coli O157:H7-specific phage as a biocontrol agent

Isolation and characterization of a novel Escherichia coli O157:H7-specific phage as a biocontrol agent

Isolation and characterization of bacteriophages that infect Citrobacter rodentium, a model pathogen for investigating human intestinal diseases

In vitro bacteriolytic activity of Salmonella specific novel isolated bacteriophage

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