Isolation and characterization of a new β-galactose-specific lectin from the sea worm Chaetopterus variopedatus

Mikheyskaya, Lidiya V.; Евтушенко, Е. В.; Оводова, Р. Г.; Belogortseva, Natalia; Ovodov, Yu. S.

doi:10.1016/0008-6215(95)00139-k

Cited by 13 publications

(10 citation statements)

References 12 publications

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“…The antiviral activities, structural classes, and glycan specificities are summarized in Tables 5 and 6 5.1. Lectin from marine worm Chaetopterus variopedatus (CVL) e structure unknown CVL was isolated from the marine worm Chaetopterus variopedatus (Mikheyskaya et al, 1995). The 30 kDa protein was reported to inhibit HIV-1 syncytium formation with an EC 50 of 4.3 nM and did not elicit any cytopathic effects in C8166 cells at the concentrations tested (0.003e1.67 mM) (Wang et al, 2006).…”

Section: Antiviral Lectins Obtained From Marine Eukaryotic Organismsmentioning

confidence: 99%

“…(kDa) Oligomeric status Structure class Specificity Glycoprotein bound References CVL 30 Unk Unk . Galactose Unk ( Mikheyskaya et al, 1995 , Wang et al, 2006 ) SVL-1 15.5 4 Unk . Mannose Unk ( Molchanova et al, 2007 , Lukyanov et al., 2007 ) SVL-2 12.7 4 Unk .…”

Section: Molecular Mechanisms Of Antiviral Lectinsmentioning

confidence: 99%

“…CVL was isolated from the marine worm Chaetopterus variopedatus ( Mikheyskaya et al., 1995 ). The 30 kDa protein was reported to inhibit HIV-1 syncytium formation with an EC 50 of 4.3 nM and did not elicit any cytopathic effects in C8166 cells at the concentrations tested (0.003–1.67 μM) ( Wang et al., 2006 ).…”

Section: Antiviral Lectins Obtained From Marine Eukaryotic Organismsmentioning

confidence: 99%

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Antiviral lectins: Selective inhibitors of viral entry

2017

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Many natural lectins have been reported to have antiviral activity. As some of these have been put forward as potential development candidates for preventing or treating viral infections, we have set out in this review to survey the literature on antiviral lectins. The review groups lectins by structural class and class of source organism we also detail their carbohydrate specificity and their reported antiviral activities. The review concludes with a brief discussion of several of the pertinent hurdles that heterologous proteins must clear to be useful clinical candidates and cites examples where such studies have been reported for antiviral lectins. Though the clearest path currently being followed is the use of antiviral lectins as anti-HIV microbicides via topical mucosal administration, some investigators have also found systemic efficacy against acute infections following subcutaneous administration.

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Section: Antiviral Lectins Obtained From Marine Eukaryotic Organismsmentioning

confidence: 99%

Section: Molecular Mechanisms Of Antiviral Lectinsmentioning

confidence: 99%

Section: Antiviral Lectins Obtained From Marine Eukaryotic Organismsmentioning

confidence: 99%

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Antiviral lectins: Selective inhibitors of viral entry

2017

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“…Despite extensive work on the development (e.g. Lillie 1902Lillie , 1906Irvine et al 1999;Irvine & Martindale 2000;Potenza et al 2003), ecology, physiology, life history (Enders 1905(Enders , 1907(Enders , 1909Petersen & Johansen 1967;Brown et al 1972;Brown 1977) and biochemistry (Mikheyskaya et al 1995;Wang et al 2006;Branchini et al 2013) of Chaetopterus, there have been only a few studies to date on the phylogenetic relationships within Chaetopteridae. Some of these have found a well-supported sistergroup relationship between Chaetopterus and Mesochaetopterus using 18S and COI data (Osborn et al 2007;Martin et al 2008), as has been previously proposed based on morphological similarity (Potts 1914).…”

Section: Introductionmentioning

confidence: 99%

Phylogenetic analyses of Chaetopteridae (Annelida)

2017

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Phylogenetic relationships within Chaetopteridae were assessed using parsimony cladistic analysis of 43 external morphological characters and molecular phylogenetic analysis of three genes: mitochondrial cytochrome oxidase subunit I DNA (COI) and nuclear 28S and 18S ribosomal DNA. The group currently comprises four accepted genera: Chaetopterus Cuvier, Spiochaetopterus Sars, Phyllochaetopterus Grube and Mesochaetopterus Potts, with 97 available species names. We selected 79 species and one subspecies with adequately detailed data for morphological comparison. Sixteen additional unidentified chaetopterid species were included to enhance representation of the morphological and genetic diversity of the family. The morphological matrix was coded chiefly from the original descriptions, as well as revisions, type specimens and newly collected specimens. Chaetopteridae was monophyletic in the molecular phylogeny, and united by a number of morphological features, including a single pair of grooved peristomial palps, three morphologically distinct tagmata (A, B, C) and the presence of stout, modified cutting chaetae on anterior chaetiger 4 (A4). Chaetopterus and Mesochaetopterus formed well‐supported sister clades based on sequence data; however, the reciprocal monophyly of these genera was not supported by morphological data. Spiochaetopterus and Phyllochaetopterus were paraphyletic/polyphyletic as currently defined, and the status of these and other available generic names is discussed.

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“…Chaetopterus variopedatus, a remarkable and specialized worm, has inspired numerous investigations [12][13][14][15][16][17][18]. It lives in Ushaped opaque parchment-like tubes and filters food from the water using a secreted mucous net.…”

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confidence: 99%

Absolute Configuration and Body Part Distribution of the Alkaloid 6-epi-Monanchorin from the Marine Polychaete Chaetopterus variopedatus

Shubina

Makarieva

Denisenko

et al. 2016

Natural Product Communications

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As a result of the first study on secondary metabolites from the cosmopolitan bioluminescent marine tube polychaete Chaetopterus variopedatus, a new bicyclic guanidine alkaloid, 6-epi-monanchorin (1), along with the previously known monanchorin (2) were isolated. The structure of 1 was elucidated by spectroscopic and chemical methods, including a cleavage of the C 1-O 7 bond to obtain a secondary alcohol (3), which was used to determine the absolute configurations by Mosher's method. It was found that 1 and 2 were mainly accumulated in a secreted mucus special organ of the worm (food net), where green and blue-green microalgae were detected. A biosynthetic pathway to 6-epi-monanchorin and monanchorin from dietary polyenic fatty acid precursors was proposed.

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Isolation and characterization of a new β-galactose-specific lectin from the sea worm Chaetopterus variopedatus

Cited by 13 publications

References 12 publications

Antiviral lectins: Selective inhibitors of viral entry

Antiviral lectins: Selective inhibitors of viral entry

Phylogenetic analyses of Chaetopteridae (Annelida)

Absolute Configuration and Body Part Distribution of the Alkaloid 6-epi-Monanchorin from the Marine Polychaete Chaetopterus variopedatus

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