Isolation and Characterization of a Plasmid fromLactobacillus fermentumConferring Erythromycin Resistance

Inhibition of HIV infectivity by a natural human isolate of Lactobacillus jensenii engineered to express functional two-domain CD4

“…Shuttle vector pOSEL144 was created by first subcloning the E. coli origin of replication from pBluescript into pVE5528 (14), and then removing the fulllength M6 coding region (PstI). This plasmid was digested with SmaI, partially digested with NdeI, filled-in by DNA polymerase I (Klenow fragment) and self-ligated.…”

“…The resulting plasmid, pOSEL144 ( Fig. 1), contains a lactobacillus-compatible origin of replication (repA) and an erythromycin resistance gene (erm) from pLEM7 (14), and has been used for the expression of heterologous proteins in a variety of lactobacillus species.…”

“…Strain Xna of L. jensenii was among a few strains selected for the expression of recombinant CD4 molecules based on these characteristics. To express heterologous proteins in this strain, an expression vector derived from a natural L. reuteri plasmid pVE5528 (14) was constructed by using a modular approach (Fig. 1).…”

Transposition in Lactobacillus delbrueckii subsp. bulgaricus: identification of two thermosensitive replicons and two functional insertion sequences

“…Plasmids used in this work were pG + host4 (Maguin et al, 1992), pG + host9 (Maguin et al, 1996), pGB305D (Le Chatelier et al, 1993, pLEM3 (Fons et al, 1997), pRL1 (Le Bourgeois et al, 1992), pRTK371 (Walker & Klaenhammer, 1994), pIL895 (Tailliez et al, 1994), pNST73 (Guedon et al, 1995) and pSKII (Stratagene). pVI1055 (M. van de Guchte, unpublished) is an E. coli-L. bulgaricus shuttle which contains the ColE1 origin, the ampicillin-resistance gene and the multicloning site derived from pSKII and the replication functions and the erythromycin-resistance gene of pGB3631 (Brantl et al, 1994).…”

Antibiotic Susceptibility of Probiotic Bacteria

“…At least 25 species of lactobacilli contain native plasmids (Wang & Lee, 1997), and often appear to contain multiple (from 1 to 16) different plasmids in a single strain. R-plasmids encoding tetracycline, erythromycin, chloramphenicol, or macrolidelincomycin-streptogramin resistance have been reported in L. reuteri (Lin et al, 1996;Tannock et al, 1994), L. fermentum (Fons et al, 1997;Ishiwa & Iwata, 1980), L. acidophilus (Vescovo et al, 1982), and L. plantarum (Danielsen, 2002) isolated from raw meat, silage and faeces. The reported prevalence of antibiotic resistance genes such as erythromycin, vancomycin, tetracycline, chloramphenicol, and gentamicin resistance genes, on transferable genetic elements in enterococci is more extensive, both on plasmids (Murray et al, 1988) and transposons (Clewell et al, 1995;Perreten et al, 1997a;Rice & Marshall, 1994;).…”