Isolation and characterization of a novel tyrosinase produced by Sahara soil actinobacteria and immobilization on nylon nanofiber membranes

Harir, Mohammed; Bellahcene, Miloud; Baratto, Maria Camilla; Pollini, Simona; Rossolini, Gian María; Trabalzini, Lorenza; Fatarella, Enrico; Pogni, Rebecca

doi:10.1016/j.jbiotec.2017.11.004

Cited by 28 publications

(27 citation statements)

References 66 publications

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“…Under the research topic of Actinobacteria as a resource of novel potential therapeutic agents, a total of 134 articles were published starting from 2002 ( Figure 2). They cover different aspects, including the isolation and diversity of bacteria along with the investigation on secondary metabolite production targeting a broad spectrum of bioalogical activities (mainly antimicrobial but also cytotoxic and plant promoting agents) and with a look at potential biotechnological applications [54]. Table 1.…”

Section: Algerian Sampling Sites Providing Culturable Actinobacteriamentioning

confidence: 99%

Actinobacteria Derived from Algerian Ecosystems as a Prominent Source of Antimicrobial Molecules

et al. 2019

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Actinobacteria, in particular “rare actinobacteria” isolated from extreme ecosystems, remain the most inexhaustible source of novel antimicrobials, offering a chance to discover new bioactive metabolites. This is the first overview on actinobacteria isolated in Algeria since 2002 to date with the aim to present their potential in producing bioactive secondary metabolites. Twenty-nine new species and one novel genus have been isolated, mainly from the Saharan soil and palm groves, where 37.93% of the most abundant genera belong to Saccharothrix and Actinopolyspora. Several of these strains were found to produce antibiotics and antifungal metabolites, including 17 new molecules among the 50 structures reported, and some of these antibacterial metabolites have shown interesting antitumor activities. A series of approaches used to enhance the production of bioactive compounds is also presented as the manipulation of culture media by both classical methods and modeling designs through statistical strategies and the associations with diverse organisms and strains. Focusing on the Algerian natural sources of antimicrobial metabolites, this work is a representative example of the potential of a closely combined study on biology and chemistry of natural products.

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Section: Algerian Sampling Sites Providing Culturable Actinobacteriamentioning

confidence: 99%

Actinobacteria Derived from Algerian Ecosystems as a Prominent Source of Antimicrobial Molecules

et al. 2019

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“…In animals, black eumelanin and reddish-yellow pheomelanin are produced by the enzymatic oxidation of tyrosine via l -3,4-dihydroxyphenylalanine ( l -dopa) to dopa-quinone. The oxidative reaction involves enzymes like tyrosinase, laccase, or tyrosine hydroxylase, depending on the organism [ 12 , 13 , 14 , 15 ]. Subsequently, non-enzymatic reactions lead to the formation of the polymer-like structure.…”

Section: Introductionmentioning

confidence: 99%

Spectroscopic Characterization of Natural Melanin from a Streptomyces cyaneofuscatus Strain and Comparison with Melanin Enzymatically Synthesized by Tyrosinase and Laccase

et al. 2018

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An actinobacteria strain was isolated from Algerian Sahara soil and assigned to Streptomyces cyaneofuscatus Pridham et al. 1958 species. This strain was selected for its ability to produce melanin exopigments in liquid and solid media. Melanin synthesis was associated with tyrosinase activity and the enzyme from this strain was isolated and biochemically characterized. Synthetic melanin was then enzymatically produced using the S. cyaneofuscatus Pridham et al. 1958 tyrosinase. As this enzyme showed a higher diphenolase activity, a synthetic melanin from the enzymic oxidation of 3,4-dihydroxyphenylalanine (dopa) was obtained by the use of a Trametes versicolor (L.) Lloyd laccase for comparison. The natural and synthetic pigments were physico-chemically characterized by the use of ultraviolet (UV)-Visible, and Fourier transform infrared (FT-IR) and multifrequency electron paramagnetic resonance (EPR) spectroscopies. All the melanin samples displayed a stable free radical when analyzed by X-band EPR spectroscopy. Once the samples were recorded at Q-band EPR, a copolymer derived from a mixture of different constituents was evident in the natural melanin. All radical species were analyzed and discussed. The use of water-soluble melanin naturally produced by S. cyaneofuscatus Pridham et al. 1958 represents a new biotechnological alternative to commercial insoluble pigments.

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“…5). In a similar study, Harir et al [11] reported the enhancing effects of preincubation of tyrosinase with Cu 2+ and Mg 2+ among other cations, which had little adverse effects overall. Fig.…”

Section: Enzyme Purification and Characterizationmentioning

confidence: 83%

“…From the kinetics determined, it could be assumed that the fungal proteins displayed a high affinity for the substrate and could transform it at a rapid rate. Furthermore, the particular uniqueness of the tyrosinases reported in this study is their relatively higher substrate affinity, compared to other sources [11,32,[34][35][36], which might imply their potential precision and efficient conversion of substrates, if applied at industrial scale. Therefore, further investigations should be encouraged to fully elucidate the potentials of these reported strains.…”

Section: Enzyme Purification and Characterizationmentioning

confidence: 87%

“…Mushroom tyrosinases have attracted much biochemical interest due to the potentials already stated, and it has encouraged the exploratory search for novel fungal sources. A novel tyrosinase producing actinomycete strain has been isolated from Sahara soil [11]. Similarly, a study has been earlier conducted on two basidiomycetes, Agaricus bisporus and Pleurotus ostreatus, which had their tyrosinases purified and characterized [9].…”

Section: Introductionmentioning

confidence: 99%

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Optimization and Characterization of Tyrosinases from Multi-enzyme Producing Fusarium solani and Fumago sp.

Odeniyi

Ogunsanya

Unuofin

2019

Period. Polytech. Chem. Eng.

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Tyrosinase is a copper-containing metalloprotein that catalyzes the oxidation of tyrosine, in particular, L-DOPA to L-Dopaquinone, which are precursors of brown pigments in some wounded eukaryotic tissues. The present study focused on screening, production and characterization of tyrosinase from multi-enzyme producing Fusarium solani B1 and Fumago sp. A total of 25 strains were isolated from rotting wood samples and screened for hydrolytic and oxidative multi-enzyme potentials using different polymeric substrates. The two most consistent strains: Fusarium solani B1 and Fumago sp. B13 were further evaluated for tyrosinase production. Some media cultural parameters and physiological conditions were optimized in order to maximize tyrosinase production. Incubation of Fumago sp. B13 and Fusarium solani B1 for 96 and 144 h in medium containing 2 % and 0.2 % ratios of Glucose and NaNO3 with pH 6 and 7, respectively, was most suitable for tyrosinase production. Characterization of the partially purified tyrosinase from Fumago sp. B13 and Fusarium solani B1 exhibited optimal activities at pH 6-7, 30 °C, and 1 mM Cu2+, respectively, thereby suggesting their potentials for novel biotechnological applications.

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Isolation and characterization of a novel tyrosinase produced by Sahara soil actinobacteria and immobilization on nylon nanofiber membranes

Cited by 28 publications

References 66 publications

Actinobacteria Derived from Algerian Ecosystems as a Prominent Source of Antimicrobial Molecules

Actinobacteria Derived from Algerian Ecosystems as a Prominent Source of Antimicrobial Molecules

Spectroscopic Characterization of Natural Melanin from a Streptomyces cyaneofuscatus Strain and Comparison with Melanin Enzymatically Synthesized by Tyrosinase and Laccase

Optimization and Characterization of Tyrosinases from Multi-enzyme Producing Fusarium solani and Fumago sp.

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