Isolation and Characterization of a Bacteriophage That Hosts on Avian-pathogenic Escherichia coli (APEC)

Fakhouri, Haya; Juneidi, Orjowan; Mohtaseb, Hana Al; Ishnaiwer, Murad; Razem, Fawzi Al-

doi:10.3923/ijps.2019.223.230

Cited by 3 publications

(8 citation statements)

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“…The larger the size of the host cell and the longer the incubation period of the phage tend to result in a larger burst size. For example, The phage SA has 30 min incubation period with 1,000 PFU/cell burst size; the phage MS2 has 45 min latent period with 2,000 PFU/cell burst size; the Avian-pathogenic E. coli phage has 24 h incubation period and 2.4 × 10 4 PFU/mL burst size ( Horiuchi and Adelberg, 1965 ; Hamza et al, 2016 ; Fakhouri et al, 2019 ). In this study, phage swi2 has a short incubation period of 25 min with a high to 1,000 PFU/cell burst size, which indicates that swi2 has potential development value for clinical application.…”

Section: Discussionmentioning

confidence: 99%

Characterization of a Novel Bacteriophage swi2 Harboring Two Lysins Can Naturally Lyse Escherichia coli

Sui

Wang

et al. 2021

Front. Microbiol.

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The novel virulent Siphoviridae bacteriophage swi2 was isolated from a pig farm, and its biological characteristics, genome architecture, and infection-related properties were characterized. Phage swi2 has a high titer of 1.01 × 1012 PFU/mL with good tolerance to UV rays and remains stable in the pH range of 6–10 and at temperatures less than 50°C. One-step growth analysis revealed that phage swi2 had a 25 min latent period with a large burst size (1,000 PFU/cell). The biological characteristics indicated that swi2 had good host infectivity and effective lytic activities. The genome of phage swi2 is composed of 47,611 bp with a G + C content of 46.50%. Eighty-nine orfs were predicted, and only 18 of them have known functions. No virulence genes or drug resistance genes were found in the genome. Genome sequence comparison of phage swi2 showed that there were a total of 10 homologous phages in the database with low similarity (less than 92.51% nucleotide identity and 66% query coverage). The predicted host lysis-related genes of phage swi2 consist of one holin, two endolysins, and Rz/Rz1 equivalents. Antibacterial activity assays showed that both endolysins could naturally reduce the host Escherichia coli 51 titers by -1 log unit both in vitro and in vivo, EDTA showed no obvious synergistic action, and holin had no lytic effects on the host cell. These results provide necessary information for the development of antibiotic alternatives for the treatment of multidrug-resistant Escherichia coli infection.

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Section: Discussionmentioning

confidence: 99%

Characterization of a Novel Bacteriophage swi2 Harboring Two Lysins Can Naturally Lyse Escherichia coli

Sui

Wang

et al. 2021

Front. Microbiol.

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“…For the isolation of S. Gallinarum specific phages , a protocol from Fakhouri et al, 2019 was followed with modifications as follows. Two ml of the above filtrate were mixed with 12 ml of LB-broth, 1 ml of an overnight S. Gallinarum culture and incubated in shaker incubator set at 160-rpm for 7 h at 37 °C.…”

Section: Methodsmentioning

confidence: 99%

“…In older flocks, the clinical signs of fowl typhoid include dehydration, diarrhea, anemia, depression, laboured breathing, decreased egg production and hatchability. Pathological changes appear in the subacute and chronic stages with small lesions, severe swelling, and discoloration in liver and spleen ( Kwon et al, 2010 , Hong et al, 2013 , Fakhouri et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

“…Antiviral and antifungal agents are rarely used in poultry as viruses and fungal diseases are usually controlled by vaccination. Bacterial diseases are controlled through the use of dozens of different antibacterial chemicals that have become common in livestock farms throughout much of the twentieth century ( Page and Gautier, 2012 , Fakhouri et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

“…It is highly possible that the inappropriate use of antibiotics in some animal husbandries may threaten their efficacy as well since antibiotics are also used to treat human pathogens ( Page and Gautier, 2012 , Fakhouri et al, 2019 , Sánchez-Salazar et al, 2020 ). For example, ceftriaxone antibiotic is used to treat Salmonella infections in children.…”

Section: Introductionmentioning

confidence: 99%

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