Isolation and characterization of a novel geminivirus from parsley

Hasanvand, Vahid; Heydanejad, Jahangir; Massumi, Hossain; Kleinow, Tatjana; Jeske, Holger; Varsani, Arvind

doi:10.1016/j.virusres.2020.198056

Cited by 10 publications

(11 citation statements)

References 38 publications

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“…ML phylogenetic analysis of the utkilio virus Rep protein together with those of other geminiviruses shows that the sequence is basal to a cluster of sequences of the genera Begomovirus , Curtovirus , Eragrovirus , Grablovirus , Opunvirus , Topocuvirus and Turncurtovirus , and the currently unclassified viruses, Opuntia virus 2 [20], Polygala garcinii-associated virus [21] and parsley yellow leaf curl virus [35] ().…”

Section: Resultsmentioning

confidence: 99%

Novel circular DNA virus identified in Opuntia discolor (Cactaceae) that codes for proteins with similarity to those of geminiviruses

Fontenele

Köhler

Majure

et al. 2021

Journal of General Virology

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Viral metagenomic studies have enabled the discovery of many unknown viruses and revealed that viral communities are much more diverse and ubiquitous than previously thought. Some viruses have multiple genome components that are encapsidated either in separate virions (multipartite viruses) or in the same virion (segmented viruses). In this study, we identify what is possibly a novel bipartite plant-associated circular single-stranded DNA virus in a wild prickly pear cactus, Opuntia discolor, that is endemic to the Chaco ecoregion in South America. Two ~1.8 kb virus-like circular DNA components were recovered, one encoding a replication-associated protein (Rep) and the other a capsid protein (CP). Both of the inferred protein sequences of the Rep and CP are homologous to those encoded by members of the family Geminiviridae. These two putatively cognate components each have a nonanucleotide sequence within a likely hairpin structure that is homologous to the origins of rolling-circle replication (RCR), found in diverse circular single-stranded DNA viruses. In addition, the two components share similar putative replication-associated iterative sequences (iterons), which in circular single-stranded DNA viruses are important for Rep binding during the initiation of RCR. Such molecular features provide support for the possible bipartite nature of this virus, which we named utkilio virus (common name of the Opuntia discolor in South America) components A and B. In the infectivity assays conducted in Nicotiana benthamiana plants, only the A component of utkilio virus, which encodes the Rep protein, was found to move and replicate systemically in N. benthamiana. This was not true for component B, for which we did not detect replication, which may have been due to this being a defective molecule or because of the model plants (N. benthamiana) used for the infection assays. Future experiments need to be conducted with other plants, including O. discolor, to understand more about the biology of these viral components.

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Section: Resultsmentioning

confidence: 99%

Novel circular DNA virus identified in Opuntia discolor (Cactaceae) that codes for proteins with similarity to those of geminiviruses

Fontenele

Köhler

Majure

et al. 2021

Journal of General Virology

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“…At the time of writing, there remain four geminivirus species that have not been assigned to any established genera: Common bean curly stunt virus, Limeum africanum associated virus, Parsley yellow leaf curl virus, and Polygala garcinii associated virus [5,15,39]. These four species are either singletons or do not group with other known geminiviruses (Figs.…”

Section: Discussionmentioning

confidence: 99%

Establishment of five new genera in the family Geminiviridae: Citlodavirus, Maldovirus, Mulcrilevirus, Opunvirus, and Topilevirus

et al. 2021

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Geminiviruses are plant-infecting, circular single-stranded DNA viruses that have a geminate virion morphology. These viruses infect both cultivated and non-cultivated monocotyledonous and dicotyledonous plants and have a wide geographical distribution. Nine genera had been established within the family Geminiviridae (Becurtovirus, Begomovirus, Capulavirus, Curtovirus, Eragrovirus, Grablovirus, Mastrevirus, Topocuvirus, and Turncurtovirus) as of 2020. In the last decade, metagenomics approaches have facilitated the discovery and identification of many novel viruses, among them numerous highly divergent geminiviruses. Here, we report the establishment of five new genera in the family Geminiviridae (Citlodavirus, Maldovirus, Mulcrilevirus, Opunvirus, and Topilevirus) to formally classify twelve new, divergent geminiviruses.

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“…As a result, it is now possible to perform exhaustive analysis of geminiviruses from cultivated and wild hosts. Divergent geminiviruses with distinct properties from most viruses in the family have thus been identified and characterised (Bernardo et al, 2013; Fontenele et al, 2020; Hasanvand et al, 2020; Varsani et al, 2009). Here we report the characterisation of malvaviscus yellow mosaic virus (MvYMV), a M. arboreus ‐infecting begomovirus containing a distinct nonanucleotide and a stem‐loop structure at the origin of replication unique in the family Geminiviridae .…”

Section: Discussionmentioning

confidence: 99%

Malvaviscus yellow mosaic virus, a divergent begomovirus carrying a nanovirus‐like nonanucleotide and a modified stem‐loop structure

Lima

Orílio

Almeida

et al. 2021

Annals of Applied Biology

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Begomoviruses (family Geminiviridae) have a circular, ssDNA genome encapsidated in twinned icosahedral particles. Numerous begomoviruses infecting noncultivated malvaceous plants have been described, and evidence suggests that they may have given rise to some of the viruses currently found in crop plants. Here we describe an atypical begomovirus infecting ornamental Turk's hat plants (Malvaviscus arboreus, Malvaceae). Total DNA was extracted, and the viral genome was amplified by rolling‐circle amplification (RCA), cloned and sequenced. Sequence and phylogenetic analyses indicated that the virus corresponds to a new begomovirus phylogenetically related to other malvaceous‐infecting begomoviruses from Brazil, and for which the name malvaviscus yellow mosaic virus (MvYMV) is proposed. M. arboreus plants inoculated with MvYMV infectious clones showed bright yellow mosaic and the virus progeny was identical to the isolate inoculated, thus fulfilling Koch's postulates. Strikingly, MvYMV has a nanovirus/alphasatellite‐like nonanucleotide sequence (5′‐TAGTATTAC‐3′). Moreover, a short sequence located 5′ of the nonanucleotide potentially forms a minor hairpin structure embedded in the major hairpin. Intramolecular interactions involving the sequence of the atypical hairpin structure were predicted and functional analyses were conducted to assess its biological relevance, revealing that the MvYMV Rep protein recognises this unique replication origin but not the typical begomovirus origin. Thus, MvYMV is a begomovirus with an atypical origin of replication and naturally found in noncultivated malvaceous plants.

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Isolation and characterization of a novel geminivirus from parsley

Cited by 10 publications

References 38 publications

Novel circular DNA virus identified in Opuntia discolor (Cactaceae) that codes for proteins with similarity to those of geminiviruses

Novel circular DNA virus identified in Opuntia discolor (Cactaceae) that codes for proteins with similarity to those of geminiviruses

Establishment of five new genera in the family Geminiviridae: Citlodavirus, Maldovirus, Mulcrilevirus, Opunvirus, and Topilevirus

Malvaviscus yellow mosaic virus, a divergent begomovirus carrying a nanovirus‐like nonanucleotide and a modified stem‐loop structure

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