Isolation and characterization of a T4‐like phage with a relatively wide host range within <i>Escherichia coli</i>

Xu, Juntian; Chen, Mianmian; He, Lingchen; Zhang, Shuqing; Ding, Tianyun; Yao, Huochun; Lu, Chengping; Zhang, Wei

doi:10.1002/jobm.201500440

Cited by 23 publications

(14 citation statements)

References 45 publications

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“…But it is also used to describe a bacteriophage that can infect multiple strains of the same species of bacteria (Vinod et al, 2006; Gupta and Prasad, 2010; Anand et al, 2015; Xu et al, 2016). As well, the term polyvalent (or polyvalence) is sometime used equivalently to broad host range although polyvalent was previously more specifically reserved for “phages active on different bacterial genera” (Ackermann and DuBow, 1987).…”

Section: Host Range Determinationmentioning

confidence: 99%

“…The basic method is to obtain an environmental sample that is likely to contain or have been in contact with the targeted host bacteria. This can be raw fecal matter (Markel and Eklund, 1974; Jensen et al, 2015; Xu et al, 2016), sewage samples (Lin et al, 2010; Khan and Nilsson, 2015; Yu et al, 2016), water samples (Uchiyama et al, 2008; Lin et al, 2011), soil samples (Campos et al, 1978; Greene and Goldberg, 1985; Anand et al, 2015), samples taken from infected or healthy humans (Ackermann et al, 1975; Ronda et al, 1981), etc. Broth media or buffer is added to the samples and they are then filtered to remove the bacteria and other solid material.…”

Section: Bacteriophage Isolation Protocolsmentioning

confidence: 99%

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More Is Better: Selecting for Broad Host Range Bacteriophages

2016

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Bacteriophages are viruses that infect bacteria. In this perspective, we discuss several aspects of a characteristic feature of bacteriophages, their host range. Each phage has its own particular host range, the range of bacteria that it can infect. While some phages can only infect one or a few bacterial strains, other phages can infect many species or even bacteria from different genera. Different methods for determining host range may give different results, reflecting the multiple mechanisms bacteria have to resist phage infection and reflecting the different steps of infection each method depends on. This makes defining host range difficult. Another difficulty in describing host range arises from the inconsistent use of the words “narrow” and especially “broad” when describing the breadth of the host range. Nearly all bacteriophages have been isolated using a single host strain of bacteria. While this procedure is fairly standard, it may more likely produce narrow rather than broad host range phage. Our results and those of others suggest that using multiple host strains during isolation can more reliably produce broader host range phages. This challenges the common belief that most bacteriophages have a narrow host range. We highlight the implications of this for several areas that are affected by host range including horizontal gene transfer and phage therapy.

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Section: Host Range Determinationmentioning

confidence: 99%

Section: Bacteriophage Isolation Protocolsmentioning

confidence: 99%

More Is Better: Selecting for Broad Host Range Bacteriophages

2016

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“…DNA isolation, genome sequencing, function prediction, and comparative genomic analysis. The phage DNA isolation of WG01 was performed according to the protocol described previously (41). The phage supernatant was collected by centrifugation at 10,000 ϫ g for 10 min and filtered through a 0.22-m Millipore filter.…”

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confidence: 99%

Alterations in gp37 Expand the Host Range of a T4-Like Phage

Chen

Zhang

Abdelgader

et al. 2017

Appl Environ Microbiol

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The use of phages as antibacterial agents is limited by their generally narrow host range. The aim of this study was to make a T4-like phage, WG01, obtain the host range of another T4-like phage, QL01, by replacing its host determinant gene region with that of QL01. This process triggered a direct expansion of the WG01 host range. The offspring of WG01 obtained the host ranges of both QL01 and WG01, as well as the ability to infect eight additional host bacteria in comparison to the wildtype strains. WQD had the widest host range; therefore, the corresponding QD fragments could be used for constructing a homologous sequence library. Moreover, after a sequencing analysis of gene37, we identified two different mechanisms responsible for the expanded host range: 1) the first generation of WG01 formed chimeras without mutations; and 2) the second generation of WG01 mutants formed from the chimeras. The expansion of the host range indicated that regions other than the C-terminal region may indirectly change the receptor specificity by altering the supportive capacity of the binding site. Additionally, we also found that the subsequent generations acquired a novel means of expanding the host range through acquiring a wider temperature range for lysis by exchanging gene37. The method developed in this work offers a quick way to change or expand the host range of a phage. Future clinical applications for screening phages against a given clinical isolate could be achieved after acquiring more suitable homologous sequences. T4-like phages have been established as safe in numerous phage therapy applications. The primary drawbacks to the use of phages as therapeutic agents include their highly specific host range. Thus, changing or expanding the host range of T4-like phages is beneficial for selecting phages for phage therapy. In this study, the host range of one T4-like phage WG01 was expanded using genetic manipulation. The WG01 derivatives acquired a novel means of expanding their host range through acquiring a wider temperature range for lysis. A region was located that had the potential to be used as a sequence region for homologous sequence recombination.

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“…Therefore, in order to understand the viral abundance, it is necessary to determine where most of their hosts are located. The most common locations of bacteria are human and animal intestines, sewage, soil, wastewater and marine environments (Abedon, 2008;Khan and Nilsson, 2015;Xu et al, 2016;Yu et al, 2016;Clokie et al, 2019).…”

Section: Resultsmentioning

confidence: 99%

Gida Kaynakli Patojen Escherichia Coli O157:h7’ye Karşi Etki̇li̇ Bakteri̇yofajlar

Şahin

Urganci

Yıldırım

2020

Gıda

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Bacteriophages are considered as alternative antibacterial agents in the food industry and phage therapy due to the widespread of multidrug-resistant pathogen bacteria. The objective of this study was to isolate lytic bacteriophages target to foodborne pathogen Escherichia coli O157:H7. For screening of bacteriophages, 51 samples were collected from sewage, wastewaters of fish farms, slaughterhouses and food factories, and 18 bacteriophages were isolated. The titer of the purified phages samples were changed among 1.7×10 4-1.02×10 8 PFU/ml. All of the isolated phages had lytic activity against E. coli O157:H7 strains and thus formed clear plaques. Four of 18 phages were found to have inhibitory effects against other E. coli strains including CFAI, ATCC 25922 and DSα in addition to E. coli O157:H7. It was observed that only two phages were infective against Salmonella Kentucky DMC35. The Eco-OH-phages were highly infection ability with EOP values from 0.5 to 0.1 against E. coli O157:H7 strains.

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Isolation and characterization of a T4‐like phage with a relatively wide host range within Escherichia coli

Cited by 23 publications

References 45 publications

More Is Better: Selecting for Broad Host Range Bacteriophages

More Is Better: Selecting for Broad Host Range Bacteriophages

Alterations in gp37 Expand the Host Range of a T4-Like Phage

Gida Kaynakli Patojen Escherichia Coli O157:h7’ye Karşi Etki̇li̇ Bakteri̇yofajlar

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