Isolation and characterization of a novel polysaccharide fromBacillus licheniformis NCIB 11634

Ebube, N. K.; Udeala, O. K.; Ghobashy, A A

doi:10.1007/bf01569630

Cited by 5 publications

(1 citation statement)

References 20 publications

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“…The MS fragmentation patterns obtained from GC generated by each peak, were subsequently analyses it shows the characteristics of aldohexoses such as glucose, galactose and mannose in sugar alditol acetate form. 23 To identify each peak, the MS was generated and then compared with the available MS of different monomers. The MS fragmentation obtained for the peak at 13.…”

Section: Gas–mass Chromatography (Gc–ms)mentioning

confidence: 99%

Exopolysaccharide production from Bacillus velezensis KY471306 using statistical experimental design

Moghannem

Farag

Shehab

et al. 2018

Brazilian Journal of Microbiology

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Exopolysaccharide (EPS) biopolymers produced by microorganisms play a crucial role in the environment such as health and bio-nanotechnology sectors, gelling agents in food and cosmetic industries in addition to bio-flocculants in the environmental sector as they are degradable, nontoxic. This study focuses on the improvement of EPS production through manipulation of different culture and environmental conditions using response surface methodology (RSM). Plackett–Burman design indicated that; molasses, yeast extract and incubation temperature are the most effective parameters. Box–Behnken RSM indicated that; the optimum concentration for each parameter was 12% (w/v) for molasses, 6 g/L yeast extract and 30 °C for incubation temperature. The most potent bacterial isolate was identified as Bacillus velezensis KY498625. After production, EPS was extracted, purified using DEAE-cellulose, identified using Fourier transform infrared (FTIR), gel permeation chromatography (GPC) and gas chromatography–mass spectroscopy (GC–MS). The result indicated that; it has molecular weight 1.14 × 105 D consisting of glucose, mannose and galactose.

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Section: Gas–mass Chromatography (Gc–ms)mentioning

confidence: 99%

Exopolysaccharide production from Bacillus velezensis KY471306 using statistical experimental design

Moghannem

Farag

Shehab

et al. 2018

Brazilian Journal of Microbiology

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Proteinase production by halophilic isolates from marine sediments

Manachini

Fortina

1994

Folia Microbiol

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Optimization of water absorbing exopolysaccharide production on local cheap substrates by Bacillus strain CMG1403 using one variable at a time approach

Muhammadi¹,

Afzal²

2014

J Microbiol.

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Optimum culture conditions, and carbon and nitrogen sources for production of water absorbing exopolysaccharide by Bacillus strain CMG1403 on local cheap substrates were determined using one variable at a time approach. Carbon source was found to be sole substrate for EPS biosynthesis in the presence of yeast extract that supported the growth only and hence, indirectly enhanced the EPS yield. Whereas, urea only coupled with carbon source could enhance the EPS production but no effect on growth. The maximum yield of EPS was obtained when Bacillus strain CMG1403 was grown statically in neutral minimal medium with 25% volumetric aeration at 30°C for 10 days. Under these optimum conditions, a maximum yield of 2.71±0.024, 3.82±0.005, 4.33±0.021, 4.73±0.021, 4.85±0.024, and 5.52±0.016 g/L culture medium was obtained with 20 g (sugar) of sweet whey, glucose, fructose, sucrose, cane molasses and sugar beet the most efficient one respectively as carbon sources. Thus, the present study showed that under optimum culture conditions, the local cheap substrates could be superior and efficient alternatives to synthetic carbon sources providing way for an economical production of water absorbing EPS by indigenous soil bacterium Bacillus strain CMG1403.

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Isolation and characterization of a novel polysaccharide fromBacillus licheniformis NCIB 11634

Cited by 5 publications

References 20 publications

Exopolysaccharide production from Bacillus velezensis KY471306 using statistical experimental design

Exopolysaccharide production from Bacillus velezensis KY471306 using statistical experimental design

Proteinase production by halophilic isolates from marine sediments

Optimization of water absorbing exopolysaccharide production on local cheap substrates by Bacillus strain CMG1403 using one variable at a time approach

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