“…Protein gel-blot analyses were carried out as described previously (Mitsuya et al, 2011). Primary [rabbit antiHvCMO peptides (Mitsuya et al, 2011) and anti-spinach BADH (Arakawa et al, 1992) IgGs] and secondary [peroxidase-conjugated donkey anti-rabbit IgG (GE Healthcare, Little Chalfont, UK)] antibodies were diluted in Can Get Signal solution (Toyobo, Osaka, Japan) at 1:1,000, 1:5,000, 1:5,000, respectively. Protein concentration was determined with the Bio-Rad Bradford Protein Assay (Bio-Rad, Hercules, CA, USA) using BSA as a standard.…”