Isolation and characterization of a bacterium able to degrade high concentrations of iprodione

Abstract

A bacterial strain CQH-1 capable of mineralizing iprodione was isolated and characterized. On the basis of its morphological, physiological, and biochemical characteristics combined with phylogenetic analysis of its 16S rRNA gene sequence, strain CQH-1 was identified as a Microbacterium sp. CQH-1. It was able to use iprodione and 3,5-dichloroaniline as the sole source of carbon and energy for its growth. It completely degraded 100 mg·L iprodione within 96 h at 30 °C. During the degradation of iprodione by strain CQH-1, 2 compounds were detected in GC-MS analysis and were recognized as N-(3,5-dichlorophenyl)-2,4-dioxoimidazolidine and 3,5-dichloroaniline. So, the biodegradation pathway of iprodione by strain CQH-1 was proposed. This is the first report of an iprodione-mineralizing strain from the genus Microbacterium, and strain CQH-1 might be a promising candidate for application in the bioremediation of iprodione-contaminated environments.

An Amidase Gene, ipaH , Is Responsible for the Initial Step in the Iprodione Degradation Pathway of Paenarthrobacter sp. Strain YJN-5

“…Generally, a mass error between Ϫ5 ppm and 5 ppm is acceptable for the identification of compounds (15). Therefore, a putative degradation pathway of iprodione in strain YJN-5 was proposed based on the results of this study and previous reports (8)(9)(10)(11)(12)(13)16) (Fig. 2F).…”

“…strain C1 was able to degrade 60 M iprodione within 8 h (10), while Microbacterium sp. strain CQH-1 degraded 0.3 mM iprodione within 96 h (13). Previously, our laboratory reported that Microbacterium sp.…”

“…strain YJN-G, and Microbacterium sp. strain CQH-1 (8)(9)(10)(11)(12)(13). The degradation pathway of iprodione has been proposed and summarized based on intermediate metabolites produced during its degradation by different strains (see Fig.…”

Biopurification system as a source of pesticide-tolerant bacteria able to degrade the commonly used pesticides chlorpyrifos and iprodione

“…Although various researchers have reported that CHL and IPR in liquid media could be used as a source of C and energy for growth [24,25], metabolites, which are more toxic than the parent compound and with antimicrobial properties to inhibit microbial growth, can be produced during microbial treatment [34]. IPR is known to inhibit DNA and RNA synthesis, cell division, and cellular metabolism in fungi; however, there is limited information that IPR may inhibit environmental microbes [35]. According to our results, Achromobacter sp.…”

“…Similar results were reported by Cao et al . [35] for a Microbacterium sp. strain CQH-1 isolated via the enrichment culture technique from a soil with previous exposure to IPR.…”