2008
DOI: 10.1111/j.1574-6968.2007.00996.x
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Isolation and characterization of a novelEnterococcus faecalisbacteriophage φEF24C as a therapeutic candidate

Abstract: Vancomycin-resistant Enterococcus faecalis (VRE) has become a significant threat in nosocomial settings. Bacteriophage (phage) therapy is frequently proposed as a potential alternative therapy for infections caused by this bacterium. To search for candidate therapeutic phages against Enterococcus faecalis infections, 30 Enterococcus faecalis phages were isolated from the environment. One of these, virulent phage phiEF24C, which has a broad host range, was selected for analysis. The plaque-forming ability of ph… Show more

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Cited by 95 publications
(108 citation statements)
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References 31 publications
(40 reference statements)
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“…ORFs 13 and 11 had the second and third greatest quantities, so they were also considered structural proteins. N-terminal processing of the major structural protein, as seen in the major structural protein of the other tailed phage, has not been observed in ORF14 of phage KHP30 (43,(63)(64)(65), although the first 3 amino acid residues of ORF13 seemed to be removed. In contrast, ORFs 3, 12, 17, and 29 were present in minor amounts.…”
Section: Resultsmentioning
confidence: 98%
See 1 more Smart Citation
“…ORFs 13 and 11 had the second and third greatest quantities, so they were also considered structural proteins. N-terminal processing of the major structural protein, as seen in the major structural protein of the other tailed phage, has not been observed in ORF14 of phage KHP30 (43,(63)(64)(65), although the first 3 amino acid residues of ORF13 seemed to be removed. In contrast, ORFs 3, 12, 17, and 29 were present in minor amounts.…”
Section: Resultsmentioning
confidence: 98%
“…Genomic DNA was extracted from the phage particles essentially as described elsewhere (43,44). Moreover, H. pylori strain NY43, which releases phage KHP30, was cultured in BEV medium and then pelleted.…”
mentioning
confidence: 99%
“…[49], vancomycin-resistant Enterococci [50][51][52], antibiotic-resistant Staphylococci [53,54], multidrugresistant Klebsiella pneumonia [55], imipenem-resistant [56,57] and multidrug-resistant Pseudomonas aeruginosa [58,59], antibiotic-resistant strains of Escherichia coli [60], and methicillin-resistant Staphylococcus aureus [61]. Today, there are several different antibacterial strategies derived from phages including enzybiotics (cloned host-specific, phageencoded lytic enzymes introduced to combat bacteria without the whole phage) and whole-phage therapy (introducing whole, viable phage to attack the infecting bacteria) [8,[62][63][64].…”
Section: Medical and Therapeutic Application Of Bacteriophagesmentioning
confidence: 99%
“…Electron microscopy, genome size estimation and nucleoside analysis were conducted to characterize the phage, according to published methods (Uchiyama et al, 2008(Uchiyama et al, , 2012Takemura-Uchiyama et al, 2013). Phage S6 was shown to belong to the family Myoviridae with a genome size of ca.…”
mentioning
confidence: 99%