Isolation and characterization of a carbon monoxide utilizing strain of the acetogen Peptostreptococcus productus

Geerligs, Gabriele; Aldrich, Henry C.; Harder, W.; Diekert, Gabriele

doi:10.1007/bf00456709

Cited by 50 publications

(21 citation statements)

References 31 publications

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“…Succinate levels were particularly enhanced with high xylose concentrations (13 mM) under CO 2 -limited conditions. Supplemental succinate, fumarate, and malate were not utilizable substrates (data not shown) (21,28).…”

Section: Effects Of Co 2 On the Metabolism Of Fructose And Xylosementioning

confidence: 96%

“…CO 2 limitation had no effect on pyruvate-dependent growth and product profiles of P. productus U-1 (Tables 1 and 2). (Note that pyruvate was not initially identified as a growthsupportive substrate for P. productus U-1 [28]; later studies demonstrated the capacity of this acetogen to utilize pyruvate [21].) However, CO 2 deprivation significantly affected glyceroldependent product profiles (Fig.…”

Section: Effects Of Co 2 On the Metabolism Of Fructose And Xylosementioning

confidence: 99%

“…Previous studies with P. productus U-1 and Marburg, which have 89% DNA homologies, have mostly concentrated on the capacities of these strains to utilize CO as an acetogenic substrate (21,28,29,41). The rumen isolates P. productus B-43 and B-116 are mixed-acid fermenters and form large amounts of acetate, lactate, and succinate from glucose under CO 2 -enriched conditions (7).…”

mentioning

confidence: 99%

“…In contrast to the rapid CO-dependent growth of strains U-1 and Marburg, strain B-43 grows poorly with CO (28). Likewise, the type strain of P. productus (ATCC 27340), isolated from a human septicemia, does not grow at the expense of CO and displays only 58% DNA homology with strain Marburg (21). On the basis of 16S ribosomal DNA analysis, the type strain has been recently reclassified as Ruminococcus productus (19).…”

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confidence: 99%

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Effect of CO2 on the fermentation capacities of the acetogen Peptostreptococcus productus U-1

Misoph

Drake

1996

J Bacteriol

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The fermentative capacities of the acetogenic bacterium Peptostreptococcus productus U-1 (ATCC 35244) were examined. Although acetate was formed from all the substrates tested, additional products were produced in response to CO 2 limitation. Under CO 2 -limited conditions, fructose-dependent growth yielded high levels of lactate as a reduced end product; lactate was also produced under CO 2 -enriched conditions when fructose concentrations were elevated. In the absence of supplemental CO 2 , xylose-dependent growth yielded lactate and succinate as major reduced end products. Although supplemental CO 2 and acetogenesis stimulated cell yields on fructose, xylose-dependent cell yields were decreased in response to CO 2 and acetogenesis. In contrast, glycerol-dependent growth yielded high levels of ethanol in the absence of supplemental CO 2 , and pyruvate was subject to only acetogenic utilization independent of CO 2 . CO 2 pulsing during the growth of CO 2 -limited fructose cultures stopped lactate synthesis immediately, indicating that CO 2 -limited cells were nonetheless metabolically poised to respond quickly to exogenous CO 2 . Resting cells that were cultivated at the expense of fructose without supplemental CO 2 readily consumed fructose in the absence of exogenous CO 2 and formed only lactate. Although the specific activity of lactate dehydrogenase was not appreciably influenced by supplemental CO 2 during cultivation, cells cultivated on fructose under CO 2 -enriched conditions displayed minimal capacities to consume fructose in the absence of exogenous CO 2 . These results demonstrate that the utilization of alternative fermentations for the conservation of energy and growth of P. productus U-1 is augmented by the relative availability of CO 2 and growth substrate.Acetogenic bacteria utilize CO 2 as a terminal electron acceptor and synthesize acetate by the acetyl coenzyme A (acetyl-CoA) Wood/Ljungdahl pathway (12,16,35,36,44). The reduction of CO 2 to acetate is coupled to respiratory conservation of energy via either membranous electron transport or sodium pumping systems (11,27,33). Although supplemental CO 2 can be essential or greatly stimulatory to acetogenesis under certain conditions, acetogens can use other respiratory reductant sinks (15,17,18), including aromatic acrylate groups (2,22,32,40), fumarate (13, 31), dimethyl sulfoxide (3), and nitrate (20,37).In contrast to the numerous studies that have evaluated the respiratory capacities of acetogens, the fermentation capacities of this bacteriological group have been minimally examined. Indeed, although certain acetogens can form fermentative end products such as ethanol (8, 39), acetogens are commonly thought to route the reductant derived from glycolysis toward the respiration of CO 2 to acetate (12,16,44). The acetogenic sewage isolate Peptostreptococcus productus U-1 has been reported to form lactate (28). Although lactate production by P. productus U-1 has not been quantitatively examined, preliminary studies in our laboratory indicated th...

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Section: Effects Of Co 2 On the Metabolism Of Fructose And Xylosementioning

confidence: 96%

Section: Effects Of Co 2 On the Metabolism Of Fructose And Xylosementioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Effect of CO2 on the fermentation capacities of the acetogen Peptostreptococcus productus U-1

Misoph

Drake

1996

J Bacteriol

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“…The dominant acetogens in the feces of horses and the ceca of rabbits were both affiliated with the Blautia group, in which the known strains Blautia hydrogenotrophica, Blautia coccoides, and Blautia schinkii can utilize H2 and CO2 to synthesize acetate as the main product [36,37]. In addition, the minimum doubling time of Blautia product with H2 and CO2 or CO as substrate is only 5 or 1.5 h [38,39], suggesting that guts of herbivores are a promising resource for harvesting novel acetogen strains with potential industrial applications.…”

Section: Discussionmentioning

confidence: 99%

Acetogen Communities in the Gut of Herbivores and Their Potential Role in Syngas Fermentation

Yang

2018

Fermentation

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Abstract:To better understand the effects of host selection on gut acetogens and their potential role in syngas fermentation, the composition and hydrogenotrophic features of acetogen populations in cow and sheep rumens, rabbit ceca, and horse feces were studied. The acetogens detected in horses and rabbits were more phylogenetically diverse than those in cows and sheep, suggesting that the host species plays an important role in shaping gut acetogen populations. Acetogen enrichments from these animals presented good capacities to use hydrogen, with acetate as the major end product. Minor propionate, butyrate, and isovalerate were also produced. During 48 h of incubation, acetogen enrichments from horse consumed 4.75 moles of H 2 to every 1 mole of acetate-significantly lower than rabbits, cows, and sheep (5.17, 5.53, and 5.23 moles, respectively) (p < 0.05)-and produced significantly more butyrate (p < 0.05). Enrichments from cows and sheep produced significantly higher amounts of propionate when compared to rabbits or horses (p < 0.05); enrichments from sheep produced the highest amounts of isovalerate (p < 0.05). These short chain fatty acids are important precursors for the synthesis of biofuel products, suggesting that gut contents of herbivores may be promising sources for harvesting functional acetogens for biofuel production.

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CO_xFixation to Elementary Building Blocks: Anaerobic Syngas Fermentation vs. Chemical Catalysis

Perret

Campos

Delgado

et al. 2022

Chemie Ingenieur Technik

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Heterogeneous catalysis and anaerobic syngas fermentation represent two different approaches for the conversion of synthesis gas into chemicals and fuels. This review provides a unique comparison of different reaction paths for the fixation of CO 2 , CO and H 2 into elementary building blocks such as methanol, acetic acid and ethanol. Operating conditions, reactor engineering, influence of gas impurities, yields, conversion efficiencies as well as downstream product recovery are compared. It was found that mass-specific productivity ranges in the same order of magnitude for both technologies, while space-time yield of heterogeneous catalysis is up to three orders of magnitude higher.

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Isolation and characterization of a carbon monoxide utilizing strain of the acetogen Peptostreptococcus productus

Cited by 50 publications

References 31 publications

Effect of CO2 on the fermentation capacities of the acetogen Peptostreptococcus productus U-1

Effect of CO2 on the fermentation capacities of the acetogen Peptostreptococcus productus U-1

Acetogen Communities in the Gut of Herbivores and Their Potential Role in Syngas Fermentation

CO_xFixation to Elementary Building Blocks: Anaerobic Syngas Fermentation vs. Chemical Catalysis

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Isolation and characterization of a carbon monoxide utilizing strain of the acetogen Peptostreptococcus productus

Cited by 50 publications

References 31 publications

Effect of CO2 on the fermentation capacities of the acetogen Peptostreptococcus productus U-1

Effect of CO2 on the fermentation capacities of the acetogen Peptostreptococcus productus U-1

Acetogen Communities in the Gut of Herbivores and Their Potential Role in Syngas Fermentation

COxFixation to Elementary Building Blocks: Anaerobic Syngas Fermentation vs. Chemical Catalysis

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CO_xFixation to Elementary Building Blocks: Anaerobic Syngas Fermentation vs. Chemical Catalysis