Isolation and characterization of a new suite of microsatellite markers in the European White Stork, Ciconia ciconia

Shephard, Jill M.; Galbusera, Peter; Hellemans, Bart; Jusic, Arnela; Akhandaf, Yasmina

doi:10.1007/s10592-008-9784-x

Cited by 12 publications

(21 citation statements)

References 10 publications

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“…Wood stork breeding colonies in the Amapá and Pantanal wetlands have moderate microsatellite diversity (Table 1). within the range observed in jabiru storks (Jabiru mycteria) in the Brazilian Pantanal (Lopes et al, 2011), Oriental storks (Ciconia boyciana) in China (Huang & Zhou, 2011) and white storks (Ciconia ciconia) in Europe (Shephard, Galbusera, Hellemans, Jusic, & Akhandaf, 2009;Shephard, Ogden, Tryjanowski, Olsson, & Galbusera, 2013). Storks therefore appear to conform to the trend of low to moderate nuclear genetic diversity described for waterbirds (mean He: 0.44 to 0.83; see Table S1 in Eo, Doyle, & DeWoody, 2011).…”

Section: Genetic Diversity Effective Population Size and Recent Desupporting

confidence: 70%

Genetic differentiation and historical demography of wood stork populations in Brazilian wetlands: Implications for the conservation of the species and associated ecosystems

Avelar

Silva

Perez

et al. 2017

Aquatic Conservation

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Wetlands are increasingly threatened by human activities worldwide. Genetic monitoring of associated wildlife provides valuable data to support their conservation. Waterbirds such as the wood stork (Mycteria americana) are good bioindicators of wetland disturbance and destruction. This study investigated past and contemporary levels of genetic diversity, differentiation and demographic processes in 236 wood storks from two major wetlands in Brazil in which breeding colonies are concentrated, using nine microsatellite loci and a 237‐bp untranslated fragment of the mitochondrial Control Region. Amapá populations (northern region) showed slightly higher levels of genetic diversity than Pantanal populations (central western region) and both populations had a low number of effective breeders. Results from assignment tests, F‐statistics, AMOVA, spatial and non‐spatial Bayesian clustering analyses support the hypothesis of gene flow among colonies within regions, but significant differentiation between regions. The better supported Bayesian coalescent models based on both markers indicated that the northern population exchanged migrants with unsampled populations, and that the central western population was founded by individuals from the north. Mitochondrial estimates revealed that the timing of population divergence broadly overlapped the Last Glacial Maximum (LGM), and that the central western population expanded more recently than the northern population. The results support the hypothesis that the coastal wetlands in northern Brazil remained stable enough to shelter large wood stork populations during the LGM and storks colonized freshwater wetlands in the central western region following deglacial warming. Conservation policies and protective measures should consider Amapá and Pantanal wood stork populations as genetically differentiated units and priority should be given to Amapá populations that represent the source gene pool. Continuous genetic monitoring of wood storks would help detect genetic signs of changes in demographic trends that may reflect alterations or degradation in wetlands.

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Section: Genetic Diversity Effective Population Size and Recent Desupporting

confidence: 70%

Genetic differentiation and historical demography of wood stork populations in Brazilian wetlands: Implications for the conservation of the species and associated ecosystems

Avelar

Silva

Perez

et al. 2017

Aquatic Conservation

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“…; Shephard et al . ; A. Ohta, 2010, unpublished data), 25 (13 from the Humboldt penguin, three from the American white pelican, five from the white stork, and four from the great white pelican) succeeded in amplifying (out of the other 64 primer pairs, 18 showed multiple bands, but 46 showed no PCR products). Out of the 25 primer pairs, four (hSTR1‐78, hSTR1‐92 and hSTR1‐150 from the Humboldt penguin and PEL086 from the great white pelican) had PCR products with different sizes among the five founders (Table ).…”

Section: Resultsmentioning

confidence: 99%

“…; we used only the B3‐2 marker for the African penguin), the white stork ( Ciconia ciconia ) (Shephard et al . ), the American white pelican ( Pelecanus erythrorhynchos ) (Hickman et al . ), and the great white pelican ( Pelecanus onocrotalus ) (De Ponte Machado et al .…”

Section: Methodsmentioning

confidence: 99%

Identification of novel genetic markers and evaluation of genetic structure in a population of Japanese crested ibis

Tsubono

Taniguchi

Matsuda

et al. 2013

Animal Science Journal

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Japanese population of the Japanese crested ibis Nipponia nippon was founded by five individuals gifted from the People's Republic of China. In order to exactly evaluate genetic structure, we first performed development of novel genetic makers using 89 microsatellite primer pairs of related species for cross-amplification. Of these, only three primer pairs were useful for the genetic markers. Additionally, we sequenced allelic PCR products of these three markers together with 10 markers previously identified. Most markers showed typical microsatellite repeat units, but two markers were not simple microsatellites. Moreover, over half of the markers did not have the same repeat units as those of the original species. These results suggested that development of novel genetic markers in this population by cross-amplification is not efficient, partly because of low genetic diversity. Furthermore, the cluster analysis by STRUCTURE program using 17 markers showed that the five founders were divided into two clusters. However, the genetic relationships among the founders indicated by the clustering seemed to be questionable, because the analysis relied largely on a small number of triallelic markers, in spite of the addition of the three useful markers. Therefore, more efficient methods for identifying large numbers of single nucleotide polymorphisms are desirable.

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“…Following extraction, each individual was genotyped at 18 polymorphic loci, seven from Shephard et al 47. ( Cc01, Cc03, Cc06, Cc07, WS03, WS14 , and WS17; 2009) and 11 discovered for this project ( Cc10, Cc15, Cc18, Cc37, Cc42, Cc44, Cc50, Cc58, Cc61, Cc69 , and Cc72 39) using PCR (for conditions see3947).…”

Section: Methodsmentioning

confidence: 99%

Extra-pair paternity in the socially monogamous white stork (Ciconia ciconia) is fairly common and independent of local density

Turjeman

Centeno‐Cuadros

Eggers

et al. 2016

Sci Rep

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Although many birds are socially monogamous, most (>75%) studied species are not strictly genetically monogamous, especially under high breeding density. We used molecular tools to reevaluate the reproductive strategy of the socially monogamous white stork (Ciconia ciconia) and examined local density effects. DNA samples of nestlings (Germany, Spain) were genotyped and assigned relationships using a two-program maximum likelihood classification. Relationships were successfully classified in 79.2% of German (n = 120) and 84.8% of Spanish (n = 59) nests. For each population respectively, 76.8% (n = 73) and 66.0% (n = 33) of nests contained only full-siblings, 10.5% (n = 10) and 18.0% (n = 9) had half-siblings (at least one nestling with a different parent), 3.2% (n = 3) and 10.0% (n = 5) had unrelated nestlings (at least two nestlings, each with different parents), and 9.5% (n = 9) and 6.0% (n = 3) had “not full-siblings” (could not differentiate between latter two cases). These deviations from strict monogamy place the white stork in the 59th percentile for extra-pair paternity among studied bird species. Although high breeding density generally increases extra-pair paternity, we found no significant association with this species’ mating strategies. Thus although genetic monogamy is indeed prominent in the white stork, extra-pair paternity is fairly common compared to other bird species and cannot be explained by breeding density.

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Isolation and characterization of a new suite of microsatellite markers in the European White Stork, Ciconia ciconia

Cited by 12 publications

References 10 publications

Genetic differentiation and historical demography of wood stork populations in Brazilian wetlands: Implications for the conservation of the species and associated ecosystems

Genetic differentiation and historical demography of wood stork populations in Brazilian wetlands: Implications for the conservation of the species and associated ecosystems

Identification of novel genetic markers and evaluation of genetic structure in a population of Japanese crested ibis

Extra-pair paternity in the socially monogamous white stork (Ciconia ciconia) is fairly common and independent of local density

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