Isolation and characterization of a complex polysaccharide from aged burley tobacco

Wright, H. E.; Burton, W.W.; Berry, R.C.

doi:10.1016/s0031-9422(00)82813-7

Cited by 7 publications

(1 citation statement)

References 5 publications

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“…It has long been known that 'quality' is positively correlated with total phenolic content (Bruckner, 1936). More recently some basis for this has been given by the finding (Wright, Burton & Berry, 1962) that cured tobacco leaf contains pigments of high molecular weight that are complexes of oxidized phenols, iron and protein. As a contribution to the chemical characterization ofleaf 'quality', the isolation ofthe pink-fluorescing phenol was undertaken.…”

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Isolation of 6-hydroxykynurenic acid from the tobacco leaf

Macnicol

1968

Biochemical Journal

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1. 6-Hydroxykynurenic acid (4,6-dihydroxyquinoline-2-carboxylic acid, 6-HKA) was isolated in crystalline form from both green and cured tobacco leaves. 2. A method for the determination of 6-HKA by paper chromatography and fluorimetry is described. 3. The content of 6-HKA in the flowers, stem and roots of the tobacco plant was much lower than that in the leaf. 4. The 6-HKA content increased throughout leaf development and senescence. 5. 6-HKA was detected in the leaves of plants representing 11 out of 27 families sampled. 6. 6-HKA was found to be devoid of antibacterial and antifungal activity, and was inactive in the Avena-coleoptile and cress-seed-germination tests. 7. The presence of 6-HKA is taken as evidence in plants of the tryptophan-catabolic pathway already known in mammals and micro-organisms.

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Isolation of 6-hydroxykynurenic acid from the tobacco leaf

Macnicol

1968

Biochemical Journal

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Fractionation of plant material II.—Amino acid composition of some fractions obtained from the broad bean plant (Vicia faba L.) and chromatographic differentiation of hydroxyproline isomers

Clarke¹,

Ellinger²

1967

J Sci Food Agric

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The amino acid compositions of three protein fractions obtained from broad bean leaves were estimated by ion exchange chromatography. Hydroxyproline was found present in the TCA-soluble, non-diffusible fraction and in the final residue after the further extraction with phenol-acetic acid-water (2 : 1 : 1, w/v/v), but not in the extract itself. By comparison with the behaviour of four authentic isomers of hydroxyproline, under two standard conditions of chromatographic analysis, the isomer found was trans-Chydroxyproline. These results are discussed in relation to other reports of hydroxyproline occurring in plant material.

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High Molecular Weight Materials of Tobacco

Chortyk

1972

The Chemistry of Tobacco and Tobacco Smoke

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Isolation and characterization of a complex polysaccharide from aged burley tobacco

Cited by 7 publications

References 5 publications

Isolation of 6-hydroxykynurenic acid from the tobacco leaf

Isolation of 6-hydroxykynurenic acid from the tobacco leaf

Fractionation of plant material II.—Amino acid composition of some fractions obtained from the broad bean plant (Vicia faba L.) and chromatographic differentiation of hydroxyproline isomers

High Molecular Weight Materials of Tobacco

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