Isolation and characterization of a novel nicotinophilic bacterium, <i>Arthrobacter</i> sp. aRF‐1 and its metabolic pathway

Ruan, Aidong; Gao, Yuan; Fang, Chao; Xu, Yaofei

doi:10.1002/bab.1682

Cited by 11 publications

(5 citation statements)

References 40 publications

(44 reference statements)

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“…strain SJY1 (Yu et al 2015 ), Arthrobacter sp. aRF- 1 (Ruan et al 2018 ), P. putida (Hu et al 2019 ), and Pseudomonas spp (Li et al 2010 ).…”

Section: Discussionmentioning

confidence: 99%

Bacillus sp. YC7 from intestines of Lasioderma serricorne degrades nicotine due to nicotine dehydrogenase

Zhang,

Yin,

Lei

et al. 2023

AMB Expr

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A large number of nicotine-containing wastes produced during the tobacco manufacturing process are seriously harmful to the environment and human health. The degradation and transformation of nicotine-containing environmental contaminants to harmless substances has become an urgent requirement. Lasioderma serricorne can grow and reproduce in nicotine-rich sources, and their intestinal microbiota show promising potential to degrade and utilize nicotine. The purpose of this study is to screen and identify nicotine-degrading bacteria from the intestines of L. serricorne and explore their degradation characteristics. A dominant strain, YC7, with significant nicotine degradation capabilities was isolated from the intestines of L. serricorne. The strain was identified as Bacillus using a polyphasic approach. The test results showed it can produce multiple enzymes that include β-glucosidase, cellulase, proteases, and amylases. The nicotine-degrading bacteria were functionally annotated using databases. Nicotine dehydrogenase (NDH) was found by combining an activity tracking test and protein mass spectrometry analysis. The YC-7 NDH in the pathway was molecularly docked and functionally verified via the gene knockdown method. The binding ability of nicotine to nicotine-degrading enzymes was investigated using molecular docking. A high-efficiency nicotine-degrading bacteria, YC-7, was isolated and screened from tobacco, and the gene functions related to degradation were verified. This investigation provides a new hypothesis for screening nicotine-degrading bacteria and increases our knowledge of potential nicotine-degrading microbial sources.

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“…strain SJY1 (Yu et al 2015 ), Arthrobacter sp. aRF- 1 (Ruan et al 2018 ), P. putida (Hu et al 2019 ), and Pseudomonas spp (Li et al 2010 ).…”

Section: Discussionmentioning

confidence: 99%

Bacillus sp. YC7 from intestines of Lasioderma serricorne degrades nicotine due to nicotine dehydrogenase

Zhang,

Yin,

Lei

et al. 2023

AMB Expr

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“…Arthrobacter was associated with spring, Kocuria and Deinococcus were associated with summer, Acinetobacter was associated with autumn, and Streptococcus was associated with winter. These bacterial taxa have been isolated from the environment as well as animals and physiologically characterized (25)(26)(27)(28)(29)(30)(31)(32). Streptococcus is a common resident of human skin.…”

Section: Discussionmentioning

confidence: 99%

Skin Microbiota of the Captive Giant Panda (Ailuropoda Melanoleuca) and the Distribution of Opportunistic Skin Disease-Associated Bacteria in Different Seasons

et al. 2021

Front. Vet. Sci.

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The giant panda is one of the rarest animals in the world. Skin diseases seriously endanger the health of giant panda and are considered the second major cause of its morbidity. Skin microbiota is a complex ecosystem, and the community structure and the pathogenic potential of bacteria on giant panda skin remain largely unclear. In order to understand the skin bacterial flora of captive giant pandas, the microbiota in giant panda skin samples collected during different seasons was profiled via 16S rRNA gene sequencing. In total, 522 genera from 53 bacterial phyla were detected, with Proteobacteria (40.5%), Actinobacteria (23.1%), Firmicutes (21.1%), Bacteroidetes (9.5%), Cyanobacteria (2.1%), and Thermi (1.2%) as the predominant phyla and Streptococcus (13.9%), Acinetobacter (9.2%), Staphylococcus (2.9%), Pseudomonas (5.9%), Dermacoccus (4.8%), Brachybacterium (2.9%), Escherichia (2.7%), Chryseobacterium (2.1%), Arthrobacter (1.6%), Kocuria (1.5%), Psychrobacter (1.2%), Deinococcus (1.1%), and Flavobacterium (1.1%) as the predominant genera. The results indicated that the diversity was lower in winter than in other seasons and higher in autumn than in other seasons, and the abundance in spring was significantly higher than that in other seasons. Several skin disease-associated bacteria were detected as opportunists in the skin microbiota of healthy giant pandas. In this study, the results indicated that the high diversity and abundance of the skin bacteria may have enhanced the occurrence of skin disease in autumn and spring and that skin disease-associated bacteria are the normal components of the skin microbiota.

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“…It is hence no surprise that this ecological niche was occupied by microorganisms that have evolved genes and pathways to utilize this compound to sustain cell growth. Bacteria that have evolved NIC degradation pathways include strains of Paenarthrobacter [103,104], Arthrobacter [105][106][107], Pseudomonas [108][109][110][111][112][113][114][115][116][117], Agrobacterium [118,119], Nocardioides [120,121], Ochrobactrum [122][123][124], Shinella [125,126], Acinetobacter [127,128], Bacillus [129,130], Sphingomonas [131,132], Pusillimonas [133], Rhodococus [121,134,135], and Ensifer [15,136].…”

Section: -Hydroxy-l-nicotine 41 Nicotine Catabolism In Bacteria As a ...mentioning

confidence: 99%

Insights into Pharmacological Activities of Nicotine and 6-Hydroxy-L-nicotine, a Bacterial Nicotine Derivative: A Systematic Review

Boiangiu,

Brinza,

Honceriu

et al. 2023

Biomolecules

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The purported cognitive benefits associated with nicotine and its metabolites in the brain are a matter of debate. In this review, the impact of the pharmacologically active metabolite of a nicotine derivative produced by bacteria named 6-hydroxy-L-nicotine (6HLN) on memory, oxidative stress, and the activity of the cholinergic system in the brain was examined. A search in the PubMed, Science Direct, Web of Science, and Google Scholar databases, limiting entries to those published between 1992 and 2023, was conducted. The search focused specifically on articles about nicotine metabolites, memory, oxidative stress, and cholinergic system activity, as well as enzymes or pathways related to nicotine degradation in bacteria. The preliminary search resulted in 696 articles, and following the application of exclusion criteria, 212 articles were deemed eligible for inclusion. This review focuses on experimental studies supporting nicotine catabolism in bacteria, and the chemical and pharmacological activities of nicotine and its metabolite 6HLN.

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Isolation and characterization of a novel nicotinophilic bacterium, Arthrobacter sp. aRF‐1 and its metabolic pathway

Cited by 11 publications

References 40 publications

Bacillus sp. YC7 from intestines of Lasioderma serricorne degrades nicotine due to nicotine dehydrogenase

Bacillus sp. YC7 from intestines of Lasioderma serricorne degrades nicotine due to nicotine dehydrogenase

Skin Microbiota of the Captive Giant Panda (Ailuropoda Melanoleuca) and the Distribution of Opportunistic Skin Disease-Associated Bacteria in Different Seasons

Insights into Pharmacological Activities of Nicotine and 6-Hydroxy-L-nicotine, a Bacterial Nicotine Derivative: A Systematic Review

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