Isolation and characterization of a heparin with high anticoagulant activity from Anomalocardia brasiliana

Dietrich, Carl P.; Paiva, JoséF.; Moraes, Carlos T.; Takahashi, Hélio K.; Porcionatto, Marimélia; Nader, Helena B.

doi:10.1016/0304-4165(85)90041-8

Cited by 55 publications

(34 citation statements)

References 25 publications

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“…These 1 H chemical shifts together with standard 1 H chemical shifts of ␤-glucuronic acids and ␣-glucosamines from the heparan sulfates described previously (24) facilitated unequivocal assignment for the majority of the cross-peaks in the 1 H/ 13 C HSQC spectrum (Fig. 5B) and subsequently fill out Table 2 with the respective 13 C chemical shifts of the novel HS from N. nodosus. These 1 H/ 13 C NMR signals show suitable agreement with the chemical shifts of other heparan sulfates from the literature with these same structural characteristics (24).…”

Section: Localization Of Glycosaminogycans In Nsupporting

confidence: 56%

“…Nonanimal sources of heparin for pharmaceutical use are currently not available. However, several authors reported the occurrence of heparin in different classes of invertebrate animals, such as crustaceans (9 -11), ascidians (12), and mollusks (13)(14)(15)(16). Heparin-like glycans with diverse disaccharide composition and anticoagulant activities varying from Ͻ5 up to 363 IU mg Ϫ1 (see Table 1) are present in marine bivalve mollusks from six families of the order Veneroida and two families from the order Pectinoida.…”

mentioning

confidence: 99%

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Unique Extracellular Matrix Heparan Sulfate from the Bivalve Nodipecten nodosus (Linnaeus, 1758) Safely Inhibits Arterial Thrombosis after Photochemically Induced Endothelial Lesion

Gomes

Kozlowski

Pomin

et al. 2010

Journal of Biological Chemistry

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Section: Localization Of Glycosaminogycans In Nsupporting

confidence: 56%

mentioning

confidence: 99%

Unique Extracellular Matrix Heparan Sulfate from the Bivalve Nodipecten nodosus (Linnaeus, 1758) Safely Inhibits Arterial Thrombosis after Photochemically Induced Endothelial Lesion

Gomes

Kozlowski

Pomin

et al. 2010

Journal of Biological Chemistry

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“…The length and abundance of these two regions vary according to the origin of heparin. Thus, bovine lung heparin is extremely rich in the region susceptible to heparinase (36), whereas bovine intestinal heparin and mollusc heparins contain significant amounts of the region susceptible to heparitinase II (37)(38)(39)(40)(41). The estimated abundance of the two regions is shown in Figure 9.…”

Section: Heparin In Invertebratesmentioning

confidence: 99%

Heparan sulfates and heparins: similar compounds performing the same functions in vertebrates and invertebrates?

Nader

Sf²,

Ea³

et al. 1999

Braz J Med Biol Res

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The distribution and structure of heparan sulfate and heparin are briefly reviewed. Heparan sulfate is a ubiquitous compound of animal cells whose structure has been maintained throughout evolution, showing an enormous variability regarding the relative amounts of its disaccharide units. Heparin, on the other hand, is present only in a few tissues and species of the animal kingdom and in the form of granules inside organelles in the cytoplasm of special cells. Thus, the distribution as well as the main structural features of the molecule, including its main disaccharide unit, have been maintained through evolution. These and other studies led to the proposal that heparan sulfate may be involved in the cell-cell recognition phenomena and control of cell growth, whereas heparin may be involved in defense mechanisms against bacteria and other foreign materials. All indications obtained thus far suggest that these molecules perform the same functions in vertebrates and invertebrates.

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“…These molecules were compared with standard dextran sulfate. The exopolysaccharides had different electrophoretic mobility for different buffer system depending on the structure of the polysaccharide (Dietrich et al, 1985).…”

Section: Discussionmentioning

confidence: 99%

Structural characterization and biological activity of exopolysaccharide from Lysinibacillus fusiformis

Mahendran¹,

Vijayabaskar²,

Saravanan³

et al. 2013

Afr. J. Microbiol. Res.

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The exopolysaccharide (EPS) of Lysinibacillus fusiformis were evaluated for their antioxidant properties. The total antioxidant capacity of EPS extracts was found to be maximum in malt medium 80.13 ± 0.26%. The hydrogen peroxide radical scavenging activity was found to be maximum in malt medium 78.3 ± 0.26%. The DPPH radical scavenging activity was found to be maximum in malt medium 48.39 ± 2.15%. 3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) inhibition assay was found to be maximum in malt medium 50.75 ± 3.85%. This study suggested that the EPS from L. fusiformis could potentially be used for antioxidant activity. Agarose gel electrophoresis was used to find the structure of mobility using acetate buffer (pH 3.6) and citrate buffer (pH 9.0). The physiochemical characterization of EPS was studied and the structure was confirmed by the Fourier transform infrared spectroscopy (FTIR), gas chromatography-mass spectrometry (GC-MS) and high performance liquid chromatography (HPLC) analysis.

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Isolation and characterization of a heparin with high anticoagulant activity from Anomalocardia brasiliana

Cited by 55 publications

References 25 publications

Unique Extracellular Matrix Heparan Sulfate from the Bivalve Nodipecten nodosus (Linnaeus, 1758) Safely Inhibits Arterial Thrombosis after Photochemically Induced Endothelial Lesion

Unique Extracellular Matrix Heparan Sulfate from the Bivalve Nodipecten nodosus (Linnaeus, 1758) Safely Inhibits Arterial Thrombosis after Photochemically Induced Endothelial Lesion

Heparan sulfates and heparins: similar compounds performing the same functions in vertebrates and invertebrates?

Structural characterization and biological activity of exopolysaccharide from Lysinibacillus fusiformis

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