Isolation and characterization of a mannan‐binding protein associated with the early chick embryo

Rutherford, Nicholas G.; Cook, G. M. W.

doi:10.1016/0014-5793(81)81224-0

Cited by 7 publications

(1 citation statement)

References 31 publications

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“…The lectins were localized on the membrane by the use of labeled (radioactive, fluorescent, or electron-dense) glycoproteins or neoglycoproteins (e.g., glycosylated albumin) in conjunction with quantitation of binding, fluorescence microscopy, flow cytofluorometry , or electron microscopy [20-231. kctins have been purified from extracts of many tissues and cells by affinity chromatography by using immobilized monosaccharides, oligosaccharides, or glycoproteins 115-211. A large group of lectins exhibiting specificity for galactosides has been characterized in various vertebrates [ 15-2 11; however, lectins with specificities for other sugars have also been described [21,24,25]. The most prevalent forms of galactosidespecific lectins are those with M,s of 14,000 and 29,OOO-34,000 [17][18][19]261.…”

Section: Mediation Of Adhesion Of Normal Cells By Endogenous Lectinsmentioning

confidence: 99%

Endogenous lectins as mediators of tumor cell adhesion

Lotan

Raz

1988

J of Cellular Biochemistry

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Endogenous carbohydrate-binding proteins have been found in various normal tissues and cells. Although lectins with different sugar-binding specificities have been described, the most prevalent ones are those that bind beta-galactosides. The ability of some normal and malignant cells to bind exogenous carbohydrate-containing ligands suggested that lectinlike activity is associated with the cell surface and that carbohydrate-binding proteins might mediate intercellular recognition and adhesion. We found that extracts of various cultured murine and human tumor cells exhibit a galactoside-inhibitable hemagglutinating activity. This activity was associated with two proteins of molecular weights of 34,000 and 14,500 daltons, which were purified by affinity chromatography by using immobilized asialofetuin. That these lectins are present on the cell surface was indicated by the binding of monoclonal antilectin antibodies to the surface of various tumor cells and by the immunoprecipitation of 125I-labeled lectins from solubilized cell-surface iodinated cells by polyclonal antilectin antibodies. That these cell surface lectins are functional was demonstrated by the ability of the galactose-terminating asialofetuin to enhance cell aggregation and of asialofetuin glycopeptides to block this homotypic aggregation as well as to suppress cell attachment to substratum, and by the inhibition of both asialofetuin-induced cell aggregation and cell attachment to substratum by the binding of monoclonal antilectin antibodies to the cell surface. These findings implicate cell surface lectins as mediators of cell-cell and cell-substratum adhesion. Some of these cellular interactions might be important determinants of tumor cell growth and metastasis.

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Section: Mediation Of Adhesion Of Normal Cells By Endogenous Lectinsmentioning

confidence: 99%

Endogenous lectins as mediators of tumor cell adhesion

Lotan

Raz

1988

J of Cellular Biochemistry

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Endogenous Lectins and Cell Adhesion in Embryonic Cells

Zalik

Milos

1986

The Cellular Basis of Morphogenesis

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Endogenous galactoside-binding lectins: a new class of functional tumor cell surface molecules related to metastasis

1987

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The formation of secondary tumors by circulating cancer cells (blood-borne metastasis) correlates with an increased tendency of the cells to form emboli by aggregation with other tumor cells or with host cells. Although it is evident that cell-cell recognition and adhesion are mediated by cell surface components, the identity of these molecules is only now being unraveled. Over the last decade an increasing number of studies have demonstrated the presence of endogenous carbohydrate-binding proteins on the surface of various normal cells, and it has been proposed that such lectin-like molecules might be involved in intercellular adhesion. We have shown that various tumor cell lines contain endogenous galactose-specific lectins. Lectin activity was detected at the cell surface by the binding of asialofetuin. This glycoprotein also enhanced the aggregation of the tumor cells. After purification by affinity chromatography on immobilized asialofetuin the lectin activity was associated with two proteins of Mr 14,500 and 34,000. By using polyclonal and monoclonal antilectin antibodies in conjunction with various immunologic techniques we have demonstrated that the endogenous lectins are present on the surface of different tumor cells. Quantitation of cell surface lectins by flow cytometric analyses of antilectin antibody binding revealed that among related tumor cells those exhibiting a higher metastatic potential expressed more lectin on their surface. The binding of monoclonal antilectin antibodies to metastatic cells decreased asialofetuin-induced homotypic aggregation in vitro and suppressed the ability of the cells to form lung metastases after intravenous injection in the tail vein of syngeneic mice. These results strongly implicate the tumor cell surface lectins in cell adhesion and metastasis. We propose that such lectins can increase the ability of tumor cells that enter the blood stream to form aggregates with other tumor cells, or to adhere to host cells or the extracellular matrix and thereby increase their metastatic potential. Other contributing components to tumor cell-host cell interactions are cell surface carbohydrate-binding proteins that have been detected on lymphocytes, platelets, macrophages, hepatocytes, and endothelial cells. These lectin-like molecules might recognize and bind carbohydrates expressed on the surface of tumor cells and enhance emboli formation and organ colonization.

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Isolation and characterization of a mannan‐binding protein associated with the early chick embryo

Cited by 7 publications

References 31 publications

Endogenous lectins as mediators of tumor cell adhesion

Endogenous lectins as mediators of tumor cell adhesion

Endogenous Lectins and Cell Adhesion in Embryonic Cells

Endogenous galactoside-binding lectins: a new class of functional tumor cell surface molecules related to metastasis

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