Isolation and Characterization of a Mouse Homolog of the Drosophila Segment Polarity Gene dishevelled

Sussman, Daniel L.; Klingensmith, John; Salinas, Patricia C.; Adams, Pamela S.; Nusse, Roel; Perrimon, Norbert

doi:10.1006/dbio.1994.1297

Cited by 166 publications

(128 citation statements)

References 31 publications

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“…3, A-C). Fetal expression of each Dvl mRNA has been previously reported (38,44,45). The isoform specificity of the three antiDvl antibodies was ascertained by immunoblotting using total cell extracts from HEK-293 cells transfected with full-length Dvl1, Dvl2, or Dvl3 cDNAs (Fig.…”

Section: Vangl2 Mutations Impair Interaction With Dishevelled Familymentioning

confidence: 98%

Independent Mutations in Mouse Vangl2 That Cause Neural Tube Defects in Looptail Mice Impair Interaction with Members of the Dishevelled Family

Torban¹,

Wang

Groulx

et al. 2004

Journal of Biological Chemistry

157

171

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Section: Vangl2 Mutations Impair Interaction With Dishevelled Familymentioning

confidence: 98%

Independent Mutations in Mouse Vangl2 That Cause Neural Tube Defects in Looptail Mice Impair Interaction with Members of the Dishevelled Family

Torban¹,

Wang

Groulx

et al. 2004

Journal of Biological Chemistry

157

171

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“…Role of Nkd1 in Spermatogenesis-It has been reported that Wnt ligands and receptors and Dvl-1 are expressed in mouse and human adult testes at high levels (29,(33)(34)(35)(36). In Drosophila, disruption of Dwnt-2 gene causes loss of the male-specific pigment cells in the reproductive tract sheath (37), indicating that Wnt signaling carries crucial functions in testicular development.…”

Section: Role Of Nkd1 In Mouse Embryonicmentioning

confidence: 99%

A Targeted Mutation of Nkd1 Impairs Mouse Spermatogenesis

Ishikawa

Miyoshi

et al. 2005

Journal of Biological Chemistry

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Nkd1 is an antagonist of the canonical Wnt/␤-catenin signaling pathway. The EF-hand motif of Nkd1 is required for its inhibitory function. Early studies suggested that Nkd1 might play important roles in mouse embryonic development and tumorigenesis. We constructed Nkd1 ؊/؊ mice whose Nkd1 protein lacked the EF-hand and was unable to inhibit Wnt/␤-catenin signaling. The homozygotes were viable and grew normally, but their fertility in males was reduced. In wild-type adult testes, Nkd1 mRNA was expressed more abundantly in the elongating spermatids than in the round spermatids. Lack of EF-hand caused reductions in the testis weight and sperm count by 30 and 60%, respectively. During testis development, Nkd1 mRNA expression started at the 25th day after birth, coincident with the onset of Wnt1 expression. Nuclear localization of ␤-catenin increased in the elongating spermatids, suggesting that the mutant Nkd1 failed to inhibit the Wnt/ ␤-catenin pathway. These results suggest that deletion of the EF-hand from Nkd1 reduces the number of the elongating spermatids at haploid stage. In contrast, the mutant Nkd1 did not affect intestinal polyposis in Apc ⌬716 mice.

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“…Three Dvl genes have been identified in mammals and all Dvl proteins contain three highly conserved regions: an amino-terminal DIX domain, a central PDZ domain and a carboxyl-terminal DEP domain [19][20][21][22]. The requirement of these domains for distinct signaling pathways varies: the DIX domain is essential for β-catenin activation, the DEP domain is implicated in the activation of the JNK pathway, while the PDZ domain is required for both [22].…”

Section: Introductionmentioning

confidence: 99%

Dishevelled interacts with p65 and acts as a repressor of NF-κB-mediated transcription

Deng

Wang

et al. 2010

Cell Res

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Dishevelled (Dvl) is a highly conserved protein family that plays an important role in mediating Wnt signaling from membrane to cytoplasm. Recently we reported that Dvl also functions in the nucleus by stabilizing the β-catenin/TCFs transcriptional complex. Here we describe that Dvl may function as a repressor of NF-κB. Our data show that Dvl directly binds to p65 and their interaction occurs in the nucleus. Dvl expression inhibits p65-mediated or TNF-α-stimulated activation of the NF-κB dependent reporter. This action of Dvl, however, is not dependent on Wnt or its downstream effector β-catenin. Chromatin immunoprecipitation assay shows that recruitment of p65 to the promoters of NF-κB target genes is significantly enhanced when expression of Dvl is knocked down. Consistently, the expression level of a subset of NF-κB target genes is also increased after knock-down of Dvl. Moreover, our data suggest that Dvl may relieve the anti-apoptotic effect of NF-κB, thus play a role in promoting apoptosis. Therefore, this work demonstrates a novel function of Dvl in modulating NF-κB-regulated gene transcription.

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Isolation and Characterization of a Mouse Homolog of the Drosophila Segment Polarity Gene dishevelled

Cited by 166 publications

References 31 publications

Independent Mutations in Mouse Vangl2 That Cause Neural Tube Defects in Looptail Mice Impair Interaction with Members of the Dishevelled Family

Independent Mutations in Mouse Vangl2 That Cause Neural Tube Defects in Looptail Mice Impair Interaction with Members of the Dishevelled Family

A Targeted Mutation of Nkd1 Impairs Mouse Spermatogenesis

Dishevelled interacts with p65 and acts as a repressor of NF-κB-mediated transcription

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