Isolation and characterization of a monoaromatic hydrocarbon-degrading bacterium,Pseudomonas aeruginosafrom crude oil

Abstract

The present study reports on the isolation and characterization of a Pseudomonas aeruginosa strain PTz-5 from crude oil from oil field sampled in Assam, India. It was capable to utilize hexadecane, benzene or toluene as a sole source of carbon aerobically. Strain PTz-5 was able to produce extracellular lipase that catalyzed triglycerides to free fatty acid and glycerol. The lipase activity was stable in the temperature range of 40 to 60 degrees C. Strain PTz-5 avidly adhered to the surface of hydrocarbon droplets during their growth in liquid culture medium. These properties could play an essential role in hydrocarbon degradation. The results presented here highlight the metabolic versatility and hydrocarbon biodegradative capability of strain PTz-5, signifying its great potential for the bioremediation of various hydrocarbon-contaminated environments.

Isolation and characterization of naphthalene biodegrading Methylobacterium radiotolerans bacterium from the eastern coastline of the Kingdom of Saudi Arabia

“…The members of the genus Pseudomonas are known to have diverse metabolic pathways and to grow using different substrates as a source of carbon. For instance, P. aeruginosa can grow in the presence of a variety of aliphatic and aromatic compounds such as lactate (Gao et aromatic fl uoranthene, phenanthrene (Zhang et al 2011), hexadecane, benzene, toluene (S Mukherjee et al 2010), paracetamol (Hu et al 2013), and 4-chlorobenzoate (Hoskeri et al 2011). Similar wide range of assimilation of substrates has been reported with other species of the same genus, such as Pseudomanas putida with biodegradation of aromatic compounds (Diaz et al 2008, Ebrahimi and Plettner 2013, El-Naas et al 2009, Fernandez et al 2012, Hwang et al 2009, Li et al 2011, Q Lin and Jianlong 2010, Phale et al 2013, Takeo et al 2006, You et al 2013) and alkane derivatives (Dunn et al 2005, Johnson and Hyman 2006, Smith and Hyman 2004.…”

IDENTIFICATION OF POTENTIAL DIESEL OIL-DEGRADING BACTERIA ISOLATED FROM MANADO SEA PORT BASED ON 16S rRNA GENE

“…According Rajasa (2004), if the percentage of homology ismore than 97% then it is the same species, between 93% and 97% belongs to the same genus with different species, and if less than 93% then there is the chance that it is a new species. Mukherjee et al (2010) reported that Pseudomonas aeruginosa isolated from crude oil at oil field in Assam, India, was capable to utilize hexadecane, benzene, or toluene as a sole carbon source aerobically. This bacterium produced extra cellular lipase that plays an essential role in hydrocarbon degradation, thus it is potential for 1000 bp 1500 bp 3000 bp 1400 bp bioremediation of various hydrocarboncontaminated environments.…”

Aerobic Degradation of Benzene by Escherichia spp. from Petroleum-contaminated Sites in Kolkata, West Bengal, India

“…Earlier reports of bacterial species capable of mineralizing benzene and other aromatic hydrocarbons, had been concentrated around oil-field and oil-refineries only [41][42][43] . In pure culture, benzene biodegradation have been reported by a number of aerobic bacterial genera like, Geobacter 44 , Desulfobacterium 45 , Dechloromonas 21 and Bacillus 46 ; and anaerobic genera like, Nitrosomonas 14 , Pseudomonas 16,17 and Acinetobacter 18 . In genus Escherichia, degradation of aromatic compounds like, hydroxyphenyl acetic acid, 3-hydroxyphenylpropionic acid; 3-hydroxycinnamic acid; and phenylacetic acid have been comprehensively studied 47 .…”

“…R e m e d i a t i o n o f g r o u n d w a t e r contaminated with benzene and other aromatic hydrocarbons has been proven to be an arduous task, due to the fact that these compounds are recalcitrant, water-insoluble and have the ability of diffusing rapidly once they are introduced into the aquifer system 13 . Mineralization of benzene has been a matter of priority for nearly three decades and microbial bioremediation of the same has been reported under aerobic conditions by genera, Nitrosomonas 14 , Pseudomonas 15,16,17 , Acinetobacter 18 , Lysinibacillus 19 as well as in the presence of other electron acceptors by genera, Geobacter 20 , Dechloromonas 21 , Desulfobacterium 22 . Studies on bacterial mineralization of aromatic compounds like benzene have been focused, and microorganisms capable of benzene biodegradation under aerobic conditions have been isolated from industrial areas, and areas adjoining oil fields and oil-refineries [22][23][24] .…”