Isolation and Characterization of a Bluegill-Degrading Microorganism, and Analysis of the Root Hair-Promoting Effect of the Degraded Products

Sanpa, Sirilak; Sumiyoshi, Sayoko; Kujira, Tadakazu; Matsumiya, Yoshiki; Kubo, Michinori

doi:10.1271/bbb.70.340

Cited by 7 publications

(2 citation statements)

References 28 publications

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“…The DNA extraction method for 16S rDNA sequencing and sequence data analysis were described previously (Sanpa et al 2006). The 16S rDNA of the isolates was amplified by polymerase chain reaction (PCR) with primers 20F (5 0 -TGTAA TCGTC GGCCA GTAGA GTTTG ATCCT GGCTC -3 0 ) and 1510R (5 0 -CAGGA AACAG CTATG ACCGG CTACC TTGTT ACGAC T-3 0 ).…”

Section: Methodsmentioning

confidence: 99%

Phylogenetic analysis of long-chain hydrocarbon-degrading bacteria and evaluation of their hydrocarbon-degradation by the 2,6-DCPIP assay

et al. 2008

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Thirty-six bacteria that degraded long-chain hydrocarbons were isolated from natural environments using long-chain hydrocarbons (waste car engine oil, base oil or the c-alkane fraction of base oil) as the sole carbon and energy source. A phylogenetic tree of the isolates constructed using their 16S rDNA sequences revealed that the isolates were divided into six genera plus one family (Acinetobacter, Rhodococcus, Gordonia, Pseudomonas, Ralstonia, Bacillus and Alcaligenaceae, respectively). Furthermore, most of the isolates (27 of 36) were classified into the genera Acinetobacter, Rhodococcus or Gordonia. The hydrocarbon-degradation similarity in each strain was confirmed by the 2,6-dichlorophenol indophenol (2,6-DCPIP) assay. Isolates belonging to the genus Acinetobacter degraded long-chain normal alkanes (n-alkanes) but did not degrade short-chain n-alkanes or cyclic alkanes (c-alkanes), while isolates belonging to the genera Rhodococcus and Gordonia degraded both long-chain n-alkanes and c-alkanes.

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Section: Methodsmentioning

confidence: 99%

Phylogenetic analysis of long-chain hydrocarbon-degrading bacteria and evaluation of their hydrocarbon-degradation by the 2,6-DCPIP assay

et al. 2008

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“…Cultures of the selected strains were analyzed for the WSP content, and those showing a higher amount (1,000 mg ml 1 ) were selected for taxonomic classification. For taxonomic classification, 16S rRNA gene sequences were analyzed following the procedures of Koma et al (2003) and Sanpa et al (2006). The 16S rRNA gene primers 20F (5 -TGTAATCGTCGGCCAG TAGAGTTTGATCCTGGCTC-3 ) and 1510R (5 -CAG were used for PCR to amplify the 16S rRNA gene.…”

Section: Methodsmentioning

confidence: 99%

Isolation and identification of phytate-degrading bacteria and their contribution to phytate mineralization in soil

Horii

Matsuno

Tagomori

et al. 2013

J. Gen. Appl. Microbiol.

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To better understand the phosphorus (P) cycling in an agricultural soil environment, amounts of total, organic and inorganic P in 10 agricultural soil samples were analyzed. Since a large proportion (57.8%) of the total P in the soils was in organic form, a method was developed to evaluate the mineralization rate of organic P in the soil by adding phytate to the soil and analyzing the change in water-soluble P (WSP) content after incubating it for 3 days. Moreover, the relationship between the phytate mineralization activity and bacterial biomass in 60 agricultural soils was also investigated, where the phytate mineralization activity ranged from 0 to 61.7% (average: 18.8%), and the R² value between phytate mineralization activity and indigenous bacterial biomass was 0.11 only. Phytate-degrading bacteria were isolated from the soil environment, and identified as Pseudomonas rhodesiae JT29, JT32, JT33, JT34, JT35, Pseudomonas sp. JT30, and Flavobacterium johnsoniae JT31. When P. rhodesiae JT29 and F. johnsoniae JT31 were inoculated into the agricultural soils, the phytate mineralization activities were increased up to 16 and 27 times, respectively. It was concluded that promotion of effective phytate-degrading bacterial strains could improve the sustainable P management in the agricultural soils.

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Effect on epidermal cell of soybean protein-degraded products and structural determination of the root hair promoting peptide

Matsumiya

Sumiyoshi

Matsukura

et al. 2007

Appl Microbiol Biotechnol

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Peptide(s) produced from degraded soybean protein by an alkaline protease from Bacillus circulans HA12 (degraded soybean-meal products; DSP) increased the number of both the root hair cells (trichoblasts) and hairless cells (atrichoblasts) of Brassica rapa by about 4.4 times and 1.9 times, respectively. To identify the root hair-promoting peptide(s) in DSP, the origin protein of the root hair-promoting peptide(s) was identified as Kunitz trypsin inhibitor (KTI). The root hair-promoting peptide in the degraded products of KTI was purified and produced a signal of 1,198.2 Da with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis. A search of the amino acid sequence of KTI located the peptide GGIRAAPTGNER, which had a molecular weight identical to 1,198.2 Da. The peptide GGIRAAPTGNER was chemically synthesized, and the synthetic peptide possessed root hair-promoting activity. Thus, it is concluded that this peptide in DSP is the foreign bioactive peptide promoting the differentiation of root hairs.

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Isolation and Characterization of a Bluegill-Degrading Microorganism, and Analysis of the Root Hair-Promoting Effect of the Degraded Products

Cited by 7 publications

References 28 publications

Phylogenetic analysis of long-chain hydrocarbon-degrading bacteria and evaluation of their hydrocarbon-degradation by the 2,6-DCPIP assay

Phylogenetic analysis of long-chain hydrocarbon-degrading bacteria and evaluation of their hydrocarbon-degradation by the 2,6-DCPIP assay

Isolation and identification of phytate-degrading bacteria and their contribution to phytate mineralization in soil

Effect on epidermal cell of soybean protein-degraded products and structural determination of the root hair promoting peptide

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