Isolation and characterization of a Drosophila gene that encodes multiple neuropeptides related to Phe-Met-Arg-Phe-NH2 (FMRFamide).

Schneider, Lynne E.; Taghert, Paul H.

doi:10.1073/pnas.85.6.1993

Cited by 231 publications

(113 citation statements)

References 32 publications

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“…The D. melanogaster FMRFamide-like peptide precursor also contains predicted peptides with FVRSamide, FIRFamide, FMHFamide and MDRYamide C-terminal motifs [48]. Mass spectrometry has only confirmed the expression of the FVRSamide, FIRFamide peptides in the CNS of this insect [43].…”

Section: Discussionmentioning

confidence: 99%

Genomic and peptidomic analyses of the neuropeptides from the emerging pest, Drosophila suzukii

2015

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Tel: +44 (0)1904 4626282 Highlights  In silico analysis identified 28 peptide precursors  Peptidome analysis of adult brain, corpus cardiacum, thoracico-abdominal ganglion identified peptides from 15 different families.  Myosuppressin was present in all tissue analysed, and was the only peptide identified in the crop nerve bundle AbstractDrosophila suzukii is a highly polyphagous invasive pest which has been recently introduced into Europe and North America, where it is causing severe economic losses through larval infestations of stone and berry fruits. The peptidome of the selected nervous tissues of adult D. suzukii was investigated as a first step in identifying potential targets for the development of novel insecticides.Through in silico analyses of the D. suzukii genome databases 28 neuropeptide families, comprising more than 72 predicted peptides were identified. Using a combination of liquid chromatography and mass spectrometry of tissue extracts, 33 predicted peptides, representing 13 different peptide families were identified by their molecular masses and a total of 17 peptide sequences were confirmed by ion fragmentation.A comparison between the peptides and precursors of D. suzukii and D. melanogaster shows they are highly conserved, with differences only identified in the amino acid sequences of the peptides encoded in the FMRFamide, hugin and ecydysis triggering hormone precursors. All other peptides predicted and identified from D. suzukii appear to be identical to those previously characterized from D. melanogaster.Adipokinetic hormone was only identified in the corpus cardiacum, other peptides present included short neuropeptide F, a pyrokinin and myosuppressin, the latter of which was the only peptide identified from the crop nerve bundle. Peptides present in extracts of the brain and/or thoracicoabdominal ganglion included allatostatins, cardioacceleratory peptide 2b, corazonin, extended FMRFamides, pyrokinins, myoinihibitory peptides, neuropeptide-like precursor 1, SIFamide, short neuropeptide F, kinin, sulfakinins and tachykinin related peptides.

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Section: Discussionmentioning

confidence: 99%

Genomic and peptidomic analyses of the neuropeptides from the emerging pest, Drosophila suzukii

2015

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“…The full complement of GPCRs has yet to be thoroughly characterized. At least 13 FMRFamide-like peptides exist in Drosophila (Schneider and Taghert, 1988), and it is therefore conceivable that other FMRFamide receptors may be found.…”

Section: Discussionmentioning

confidence: 99%

Peptide-Induced Modulation of Synaptic Transmission and Escape Response inDrosophilaRequires Two G-Protein-Coupled Receptors

Klose¹,

Dason²,

Atwood³

et al. 2010

J. Neurosci.

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Neuropeptides are found in both mammals and invertebrates and can modulate neural function through activation of G-protein-coupled receptors (GPCRS).The precise mechanisms by which many of these GPCRs modulate specific signaling cascades to regulate neural function are not well defined. We used Drosophila melanogaster as a model to examine both the cellular and behavioral effects of DPKQDFMRFamide, the most abundant peptide encoded by the dFMRF gene. We show that DPKQDFMRFamide enhanced synaptic transmission through activation of two G-protein-coupled receptors, Fmrf Receptor (FR) and Dromyosupressin Receptor-2 (DmsR-2). The peptide increased both the presynaptic Ca 2ϩ response and the quantal content of released transmitter. Peptide-induced modulation of synaptic function could be abrogated by depleting intracellular Ca 2ϩ stores or by interfering with Ca 2ϩ release from the endoplasmic reticulum through disruption of either the ryanodine receptor or the inositol 1,4,5-trisphosphate receptor. The peptide also altered behavior. Exogenous DPKQDFMRFamide enhanced fictive locomotion; this required both the FR and DmsR-2. Likewise, both receptors were required for an escape response to intense light exposure. Thus, coincident detection of a peptide by two GPCRs modulates synaptic function through effects of Ca 2ϩ -induced Ca 2ϩ release, and we hypothesize that these mechanisms are involved in behavioral responses to environmental stress.

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“…7 The fmrf gene products are widely distributed in the larval and adult Drosophila CNS. In total, 17 different DrmFMRFamide cell types might exist.…”

Section: The Fmrfamide Precursormentioning

confidence: 99%

Peptidomic analysis of the larval Drosophila melanogaster central nervous system by two‐dimensional capillary liquid chromatography quadrupole time‐of‐flight mass spectrometry

et al. 2005

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Peptides are the largest class of signalling molecules found in animals. Nevertheless, in most proteomic studies peptides are overlooked since they literally fall through the mazes of the net. In analogy with proteomics technology, where all proteins expressed in a cell or tissue are analyzed, the peptidomic approach aims at the simultaneous visualization and identification of the whole peptidome of a cell or tissue, i.e. all expressed peptides with their post-translational modifications. In this paper we describe the analysis of the larval fruit fly central nervous system using two-dimensional capillary liquid chromatography/quadrupole time-of-flight tandem mass spectrometry (LC/Q-TOF-MS/MS. Using the central nervous systems of only 50 larval Drosophila as starting material, we identified 38 peptides in a single analysis, 20 of which were not detected in a previous study that reported on the one-dimensional capillary LC/MS/MS analysis of the same tissue. Among the 38 sequenced peptides, some originate from precursors, such as the tachykinin and the IFamide precursor that were entirely missed in the first study. This clearly demonstrates that the two-dimensional capillary LC approach enhances the coverage of the peptidomic analysis.

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Isolation and characterization of a Drosophila gene that encodes multiple neuropeptides related to Phe-Met-Arg-Phe-NH2 (FMRFamide).

Cited by 231 publications

References 32 publications

Genomic and peptidomic analyses of the neuropeptides from the emerging pest, Drosophila suzukii

Genomic and peptidomic analyses of the neuropeptides from the emerging pest, Drosophila suzukii

Peptide-Induced Modulation of Synaptic Transmission and Escape Response inDrosophilaRequires Two G-Protein-Coupled Receptors

Peptidomic analysis of the larval Drosophila melanogaster central nervous system by two‐dimensional capillary liquid chromatography quadrupole time‐of‐flight mass spectrometry

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