Isolation and characterization of a novel human bladder cancer cell line: BK10

Roberson, Kathryn M.; Yancey, Donna; Padilla‐Nash, Hesed; Edwards, Darryl W.; Nash, William G.; Jacobs, S.; Padilla, Geraldine; Larchian, William A.; Robertson, Cary N.

doi:10.1007/s11626-998-0113-y

Cited by 5 publications

(6 citation statements)

References 35 publications

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“…Out of the 4 cases of surgical samples of bladder cancer harvested in the interim, we were unable to establish any primary cell lines using DMEM or RPMI-1640 media supplemented with fetal bovine serum. Previous reports have documented the need to supplement with additional growth factors, such as fibroblast growth factor (FGF) 47 , 48 . At this juncture, we have not optimized the culturing conditions to establish a primary bladder cancer cell line.…”

Section: Resultsmentioning

confidence: 99%

Establishment of xenografts of urological cancers on chicken chorioallantoic membrane (CAM) to study metastasis

Hu¹,

Ishihara²,

Chin

et al. 2019

Precision Clinical Medicine

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Cancer of the urological system commonly occurs in the kidney, bladder, and prostate gland. The clear cell subtype of renal cell carcinoma (ccRCC) constitutes the great majority of kidney cancer. Metastatic ccRCC portends a very poor outcome with no effective treatment available. Prostate cancer is the most common cancer in males in the US. Despite recent advances in selective kinase inhibitors and immunotherapies, the rate of developing new treatment from bench to bedside is slow. A time-consuming step is at the animal drug testing stage, in which the mouse model is the gold standard. In the pursuit to streamline the in vivo cancer biology research and drug development, we explored the feasibility of the chicken chorioallantoic membrane (CAM) model to establish xenografts. The CAM model greatly shortens the time of tumor growth and lowers the cost comparing to immunocompromised mice. We generated CAM xenografts from ccRCC, bladder and prostate cancer, with established cancer cell lines and freshly isolated patient-derived tissues, either as primary tumor cells or small pieces of tumors. The successful CAM engraftment rate from the different tumor sources is 70% or above. Using our previously established metastatic ccRCC mouse model, we showed that the CAM xenograft maintains the same tumor growth pattern and metastatic behavior as observed in mice. Taken together, CAM can serve as a valuable platform to establish new patient-derived xenografts (PDXs) to study tumor biology, thus accelerating the development of individualized treatment to halt the deadly metastatic stage of cancer.

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Section: Resultsmentioning

confidence: 99%

Establishment of xenografts of urological cancers on chicken chorioallantoic membrane (CAM) to study metastasis

Hu¹,

Ishihara²,

Chin

et al. 2019

Precision Clinical Medicine

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“…G-banding analysis of BK-10 identified 15 translocations in clone A and 18 in clone B. Multiple copies of chromosomes 9 and 20 were observed (Roberson et al, 1998).…”

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confidence: 98%

“…Image acquisition for SKY was performed as previously described . Twenty SKY images with their companion inverted-DAPI images were analyzed and compared to 20 G-banded karyotypes prepared in our initial study (Roberson et al, 1998).…”

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confidence: 99%

“…The potential of integrating banding and painting information is presented in Figure 2 for clone B using the large der(1q) marker as an example (Table 1). In order to determine breakpoints of the aberrations, inverted-DAPI chromosomes were aligned next to their respective SKY classified images and subsequently compared to their G-banded counterparts, taken from images produced in the previous G-band analysis (Roberson et al, 1998). The origin of all components of this marker could not be accurately identified with G-banding alone.…”

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Molecular cytogenetic analysis of the bladder carcinoma cell line BK-10 by spectral karyotyping

Padilla‐Nash

Nash

Padilla

et al. 1999

Genes Chromosom. Cancer

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The bladder cancer cell line BK‐10 was established from a grade III–IV transitional cell carcinoma (TCC). BK‐10 is near‐tetraploid (±4n) and consists of two subclones with 20–25 structural aberrations. Here we report the cytogenetic analysis of BK‐10 by G‐banding, spectral karyotyping (SKY), and FISH. SKY refers to the hybridization of 24 differentially labeled chromosome painting probes and the simultaneous visualization of all human chromosomes using spectral imaging. SKY enabled us to confirm 12 markers in BK‐10 previously described by G‐banding, redefine 11 aberrations, and detect 4 hidden chromosomal rearrangements, 2 of which had been identified as normal or deleted copies of chromosome 20 and 1 as a normal chromosome 3. Twenty out of 21 translocations identified were unbalanced. FISH analysis of BK‐10 using chromosome arm‐specific paints, centromere probes, and oncogene/tumor suppressor gene‐specific probes revealed a deletion of CDKN2A (p16) in all copies of chromosome 9, a low‐level amplification of MYC (five copies), and loss of one copy of TP53; detected the presence of the Y chromosome in a hidden translocation; and detected four copies of ERBB‐2. A probe set for BCR and ABL verified breakpoints for all translocations involving chromosomes 9 and 22. A new karyotype presentation, “SKY‐gram,” is introduced by combining data from G‐banding, SKY, and FISH analysis. This study demonstrates the approach of combining molecular cytogenetic techniques to characterize fully the multiple complex chromosomal rearrangements found in the bladder cancer cell line BK‐10, and to refine the chromosomal breakpoints for all translocations. Genes Chromosomes Cancer 25:53–59, 1999 Published 1999 Wiley‐Liss, Inc.

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“…The culture conditions of the cell lines-except for BK-10 ( Roberson et al, 1998)are described by the ATTC. Cell lines were harvested for metaphase chromosomes by mitotic shake-off following Colcemid treatment (0.025 g/ ml, 1-4 hr).…”

Section: Description Of Cell Lines and Metaphase Preparationmentioning

confidence: 99%

Jumping translocations are common in solid tumor cell lines and result in recurrent fusions of whole chromosome arms

Padilla‐Nash

Heselmeyer‐Haddad

Wangsa

et al. 2001

Genes Chromosom. Cancer

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Jumping translocations (JTs) and segmental jumping translocations (SJTs) are unbalanced translocations involving a donor chromosome arm or chromosome segment that has fused to multiple recipient chromosomes. In leukemia, where JTs have been predominantly observed, the donor segment (usually 1q) preferentially fuses to the telomere regions of recipient chromosomes. In this study, spectral karyotyping (SKY) and FISH analysis revealed 188 JTs and SJTs in 10 cell lines derived from carcinomas of the bladder, prostate, breast, cervix, and pancreas. Multiple JTs and SJTs were detected in each cell line and contributed to recurrent unbalanced whole-arm translocations involving chromosome arms 5p, 14q, 15q, 20q, and 21q. Sixty percent (113/188) of JT breakpoints occurred within centromere or pericentromeric regions of the recipient chromosomes, whereas only 12% of the breakpoints were located in the telomere regions. JT breakpoints of both donor and recipient chromosomes coincided with numerous fragile sites as well as viral integration sites for human DNA viruses. The JTs within each tumor cell line promoted clonal progression, leading to the acquisition of extra copies of the donated chromosome segments that often contained oncogenes (MYC, ABL, HER2/NEU, etc.), consequently resulting in tumor-specific genomic imbalances. Published 2001 Wiley-Liss, Inc.

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Isolation and characterization of a novel human bladder cancer cell line: BK10

Cited by 5 publications

References 35 publications

Establishment of xenografts of urological cancers on chicken chorioallantoic membrane (CAM) to study metastasis

Establishment of xenografts of urological cancers on chicken chorioallantoic membrane (CAM) to study metastasis

Molecular cytogenetic analysis of the bladder carcinoma cell line BK-10 by spectral karyotyping

Jumping translocations are common in solid tumor cell lines and result in recurrent fusions of whole chromosome arms

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