Isolation and characterization of 32 microsatellite loci for topmouth culter (Culter alburnus Basilewsky)

Abstract

ABSTRACT. The topmouth culter (Culter alburnus) is an economically important freshwater fish, which is widely distributed throughout large rivers, reservoirs, and lake areas of China. We report here the isolation and characterization of 32 new polymorphic microsatellite loci isolated from genomic DNA in this species enriched by (CA) 12 and (GA) 12 probes. The variability of these microsatellites was tested on 30 individuals cultured. The average allele number was 6.6 per locus, ranging from 3 to 12. The observed heterozygosity was from 0.4667 to 0.9000, and the expected heterozygosity was from 0.6163 to 0.9085. After using Bonferroni's correction for multiple tests, there was no evidence of linkage disequilibrium between pairs of loci, but deviations from HardyWeinberg equilibrium were found in 3 loci. These microsatellites can be used to study QTL of economic importance, population genetic diversity and the construction of genetic maps for C. alburnus in the future.

Developing Microsatellite Multiplex PCR Panels for Topmouth Culter (Culter alburnus) and their Application in Parentage Assignment

“…We revealed that Na, Ho and He ranged from 7 to 22, 0.39 to 0.75, and 0.57 to 0.91, respectively. Genetic diversity parameters of microsatellite loci in C. alburnus were slightly higher in our study than previously reported (Liu et al, 2014), but lower than those reported in another study (Fu et al, 2013). It should be noted that the individuals tested and the detection methods were related to the diversity parameters (Liu et al, 2009).…”

“…The original sequences released on GenBank database (Liu et al, 2014) were chosen for designing primers. Novel primers were designed for microsatellite loci of differing product sizes.…”

“…Despite its ecological and economic importance, studies on topmouth culter genetics remain limited (Guo et al, 2018;Liu et al, 2014;Wang et al, 2007) and selection breeding programs non-existent. Therefore, in this study, we developed three multiplex PCR panels involving twelve microsatellite loci with redesigned primers based on released sequences on GenBank database, and conducted parentage analysis using parent and offspring samples from four independent artificial breeding pilots.…”

Effect of dissolved oxygen and ammonia nitrogen on Culter alburnus: Physiology, biochemistry, and molecular analyses

“…Culter alburnus (topmouth culter) is an economically valuable freshwater fish species in China [ 9 ]. C. alburnus are widely distributed in various water systems and affiliated lakes in the Yangtze River Basin [ 10 ]. However, insufficient tolerance to hypoxia and AN is an obstacle to the industrialisation of C. alburnus .…”

Chromosome-scale assembly and quantitative trait locus mapping for major economic traits of the Culter alburnus genome using Illumina and PacBio sequencing with Hi-C mapping information

“…To date, research on C. alburnus has mainly focused on nutrition, growth, artificial reproduction, pond culture, and genetic diversity ( Liu et al, 2014 ; Xiao et al, 2014 ; Sun et al, 2015 ). From the perspective of breeding, although C. alburnus has not shown an obvious economic decline phenomenon, molecular studies on the genetic diversity of breeding populations have shown that the genetic diversity of C. alburnus is at a low level ( Qi et al, 2013 ; Qi et al, 2015 ).…”