Isolation and characterization of 15 microsatellite markers from wild tea plant (Camellia taliensis) using FIASCO method

Abstract

Camellia taliensis is one of the most important wild tea plants in China, especially in Yunnan Province. In this study, we described the development of 15 microsatellite markers from the genome of C. taliensis using the protocol of fast isolation by AFLP of sequences containing repeats (FIASCO). Polymorphism of each locus was assessed in 24 samples collected from six wild populations of C. taliensis. The average allele number of the microsatellites was four per locus, ranging from 2 to 7. The observed and expected heterozygosities varied from 0.076 to 0.5833 and from 0.1560 to 0.6917, respectively. Crossspecies amplification in other three tea plants showed eleven of them holding promise for sister species. These polymorphic SSR markers would be useful tools for population genetics studies and assessing genetic variations to establish conservation strategy, molecular identification and molecular breeding on this tea plant and its allied species and varieties in section Thea genus Camellia.

Rapid Microsatellite Isolation from a Butterfly by De Novo Transcriptome Sequencing: Performance and a Comparison with AFLP-Derived Distances

“…, [14]–[17]). However, microsatellites isolated from EST libraries differ from those typically found in regions of the genome unassociated with genes.…”

Molecular Markers

“…Subsequently, SSR markers had been used by several workers for cultivar identifications, study of genetic diversity of tea as well as wild Camellia species (Table 6.2). At certain occasions, SSRs had been mined in silico and validated either on diverse genotypes of tea or tested for their cross transferability on different species of Camellia (Yang et al 2009). …”

Isolation and characterization of microsatellite markers for Axonopus compressus (Sw.) Beauv. (Poaceae) using 454 sequencing technology

“…Fast isolation by amplified fragment length polymorphism (AFLP) of sequences containing repeats (FIASCO) has been proven to be the most effective magnetic bead enrichment method for isolating SSR markers (Sun et al, 2008;Yang et al, 2009;Hou et al, 2011;Wang et al, 2014a). However, this method is laborious and difficult to use, and cannot obtain a large number of SSRs in one experiment (Wang et al, 2014b).…”