Isolation and characterization of 15 microsatellite loci for the Japanese gudgeon<i> Sarcocheilichthys variegatus</i>

Fujita, Sari; Komiya, Takefumi; Takeshima, Hirohiko; Watanabe, Kinzo; Nishida, Mutsumi

doi:10.1111/j.1755-0998.2008.02303.x

Cited by 2 publications

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“…PCR conditions for each locus are described in Fujita et al [28]. PCR products were sized on an automated DNA sequencer (ABI Prism GA310; Applied Biosystems, Foster City, CA) using GeneScan version 3.1 (ABI) and ROX400HD as the size standard (ABI).…”

Section: Methodsmentioning

confidence: 99%

A Novel Resource Polymorphism in Fish, Driven by Differential Bottom Environments: An Example from an Ancient Lake in Japan

2011

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Divergent natural selection rooted in differential resource use can generate and maintain intraspecific eco-morphological divergence (i.e., resource polymorphism), ultimately leading to population splitting and speciation. Differing bottom environments create lake habitats with different benthos communities, which may cause selection in benthivorous fishes. Here, we document the nature of eco-morphological and genetic divergence among local populations of the Japanese gudgeon Sarcocheilichthys (Cyprinidae), which inhabits contrasting habitats in the littoral zones (rocky vs. pebbly habitats) in Lake Biwa, a representative ancient lake in East Asia. Eco-morphological analyses revealed that Sarcocheilichthys variegatus microoculus from rocky and pebbly zones differed in morphology and diet, and that populations from rocky environments had longer heads and deeper bodies, which are expected to be advantageous for capturing cryptic and/or attached prey in structurally complex, rocky habitats. Sarcocheilichthys biwaensis, a rock-dwelling specialist, exhibited similar morphologies to the sympatric congener, S. v. microoculus, except for body/fin coloration. Genetic analyses based on mitochondrial and nuclear microsatellite DNA data revealed no clear genetic differentiation among local populations within/between the gudgeon species. Although the morphogenetic factors that contribute to morphological divergence remain unclear, our results suggest that the gudgeon populations in Lake Biwa show a state of resource polymorphism associated with differences in the bottom environment. This is a novel example of resource polymorphism in fish within an Asian ancient lake, emphasizing the importance and generality of feeding adaptation as an evolutionary mechanism that generates morphological diversification.

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Section: Methodsmentioning

confidence: 99%

A Novel Resource Polymorphism in Fish, Driven by Differential Bottom Environments: An Example from an Ancient Lake in Japan

2011

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“…A total of 254 specimens from 15 localities, including 153 S. v. microoculus and 28 S. biwaensis specimens from 10 sites in/around Lake Biwa and 73 S. v. variegatus from 5 sites in western Japan, were used for the microsatellite analysis (Table 1). A total of 14 microsatellite loci isolated from S. v. microoculus (Fujita et al 2008) were analyzed, including 13 dinucleotide repeats (Svm03,Svm10,Svm32,Svm34,Svm46,Svm48,Svm49,Svm50,Svm53,Svm56,Svm72,Svm82,and Svm166) and one trinucleotide repeat (Svm51).…”

Section: Microsatellite Analysesmentioning

confidence: 99%

“…PCR conditions for each locus were described by Fujita et al (2008). PCR products were sized on an automated DNA sequencer (ABI Prism GA310; Applied Biosystems, Foster City, CA, USA) using GeneScan v3.1 and ROX400HD as the size standard (Applied Biosystems).…”

Section: Microsatellite Analysesmentioning

confidence: 99%

Multiple colonizations of Lake Biwa by Sarcocheilichthys fishes and their population history

2013

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Rapid adaptation and speciation have not been well documented for organisms in Lake Biwa, Japan, the oldest ancient lake in East Asia. To examine these processes, we focused on the divergence of Sarcocheilichthys gudgeons and conducted phylogeographic and population genetic analyses using mitochondrial DNA sequences and microsatellite data. No remarkable genetic differentiation was found between two species, S. biwaensis and S. variegatus microoculus, both endemic to Lake Biwa, whereas this species group, including S. v. variegatus, was genetically divided into three major regional groups: the Lake Biwa, Ise Bay, and western groups. Divergent mtDNA haplotypes were included within the Lake Biwa and western groups, strongly suggesting secondary contacts among allopatrically isolated populations. Dating for mtDNA phylogeny using a geological constraint suggested the colonization of Lake Biwa by multiple Sarcocheilichthys lineages that diverged from each other in the early-middle Pleistocene. Coalescent-based population analyses indicated that the local populations colonized the rocky bottom habitat in Lake Biwa from other habitats after the Last Glacial Maximum, likely reflecting past environmental changes in the lake, including the disappearance of rocky areas during the glacial climate. Divergent morphological adaptation in Sarcocheilichthys associated with substrate type may have rapidly proceeded along with environmental changes. Keywords Ancient lake • Central Japan • Gobioninae • Historical demography • Phylogeography • Trophic polymorphism (73%) haplotype (Fig. 2). A subclade of clade A, consisting of A15 and A16, and a tip haplotype, A18, were detected exclusively from the Chugoku region. Clade B haplotypes were obtained from the Lake Biwa, Kinki, and Chugoku regions, but showed some regional distribution patterns. The most interior haplotype of clade C (C03) was exclusively found in Kyushu, but the tip haplotypes were shared by specimens from the Lake Biwa, Kinki, and Chugoku regions. Clade D was endemic to the Yura River system (Kinki), and clade E was the earliest divergent group and was endemic to the Ise Bay area (Figs. 2, 3). Node tMRCA*(mean*±*SE) 95%*HPD

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Isolation and characterization of 15 microsatellite loci for the Japanese gudgeon Sarcocheilichthys variegatus

Cited by 2 publications

References 2 publications

A Novel Resource Polymorphism in Fish, Driven by Differential Bottom Environments: An Example from an Ancient Lake in Japan

A Novel Resource Polymorphism in Fish, Driven by Differential Bottom Environments: An Example from an Ancient Lake in Japan

Multiple colonizations of Lake Biwa by Sarcocheilichthys fishes and their population history

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