Isolation and characterization of 14 polymorphic microsatellite markers for the blue and red shrimp, <i>Aristeus antennatus</i> (Crustacea, Decapoda)

Cannas, Rita; Buccoli, Simona; Sacco, Flavio; Marcias, Sandro; Salvadori, Susanna; Cau, Angelo; Deiana, A. M.

doi:10.1111/j.1755-0998.2008.02183.x

Cited by 7 publications

(14 citation statements)

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“…To avoid redundancy with previous reported microsatellites, a BLASTn search employing our entire database and using a significance threshold of e -values <10 −10 was performed against all published A. antennatus microsatellites ( Cannas et al , 2008 ). All contigs matching with already available A. antennatus microsatellites were not included in the further microsatellite development.…”

Section: Methodsmentioning

confidence: 99%

“…Mitochondrial DNA (mtDNA) diversity analyses of blue and red shrimp populations on a broad oceanographic scale have detected four genetic stocks: the Western Mediterranean Sea (WM), the Eastern Mediterranean Sea (EM), the Atlantic Ocean (AO) and the Indian Ocean (IO), but have failed to distinguish genetic divergence within the regional level ( Maggio et al , 2009 ; Roldán et al , 2009 ; Fernández et al , 2011 ; Marra et al , 2015 ). Surveys using a small number of microsatellites isolated using the FIASCO protocol ( Zane, Bargelloni & Patarnello , 2002 ) failed to distinguish any type of population structure within Italian fishing grounds ( Cannas et al , 2008 ; Cannas et al , 2012 ).…”

Section: Introductionmentioning

confidence: 96%

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Development and characterization of novel microsatellite markers by Next Generation Sequencing for the blue and red shrimpAristeus antennatus

Heras

Planella

Caldarazzo

et al. 2016

PeerJ

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The blue and red shrimp, Aristeus antennatus, is a commercially important crustacean, in the Mediterranean Sea, which has been listed as a priority species for fishery management. Hypervariable microsatellite markers could be a useful tool to identify genetic stocks among geographically close fishing grounds. Potential microsatellite markers (97) identified from next-generation sequencing of an individual shrimp using a 454 GS Junior Pyrosequencer were tested on a preliminary panel of 15 individuals representing the four worldwide genetic stocks of the species from which 35 polymorphic loci were identified and used to characterize an additional 20 individuals from the Western Mediterranean Sea. In the Western Mediterranean sample, 32 out of 35 were polymorphic loci and the number of alleles per locus ranged from 2 to 14 and expected heterozygosity ranged from 0.050 to 0.968. No linkage disequilibrium was detected, indicating the independence of the loci. These novel microsatellites provide additional tools to address questions relating to genetic diversity, parentage studies and connectivity patterns of A. antennatus populations and help develop effective strategies to ensure long-term sustainability of this resource.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 96%

Development and characterization of novel microsatellite markers by Next Generation Sequencing for the blue and red shrimpAristeus antennatus

Heras

Planella

Caldarazzo

et al. 2016

PeerJ

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“…1988) with minor modifications, and resuspended in ddH 2 O. Individual genotypes were obtained using eight polymorphic microsatellite loci (Table 2) and following PCR conditions as in Cannas et al. (2008).…”

Section: Methodsmentioning

confidence: 99%

“…Total DNA was extracted following the 'Salting Out' protocol (Miller et al 1988) with minor modifications, and resuspended in ddH 2 O. Individual genotypes were obtained using eight polymorphic microsatellite loci (Table 2) and following PCR conditions as in Cannas et al (2008). PCR products together with a ROX 400 size standard underwent capillary electrophoresis at BMR-Genomics Laboratories in Padova, Italy (http://www.bmr-genomics.com).…”

Section: Tissue Sampling Dna Extraction and Microsatellite Amplificamentioning

confidence: 99%

Genetic variability of the blue and red shrimp Aristeus antennatus in the Western Mediterranean Sea inferred by DNA microsatellite loci

et al. 2011

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Genetic variation at eight microsatellite loci was studied in nine populations of the blue and red shrimp Aristeus antennatus to investigate whether distinct stocks are present in the Western Mediterranean Sea. A high level of gene flow and no evidence of genetic partitioning were discovered. No significant variation was found (FST = 0.00673, P‐value = 0.067) even when shrimps from exploited and those from deep‐water unexploited grounds were compared. No evidence of reduction or expansion of population size in the recent past was found, as indicated by the bottleneck and interlocus g‐tests. Our results are consistent with previous studies using mitochondrial gene methods and allozymes, indicating that, for this species, extensive pelagic larval dispersal and adult migration are probably responsible for the genetic homogeneity observed. In particular, due to a different bathymetric distribution of males and females, reported to be associated with different water masses and hence with possible differential dispersal capacity between sexes, the hypothesis of sex‐biased dispersal was tested. Mean values of corrected assignment indices and mean relatedness values were higher for males, suggesting that females are the more widely dispersing sex. Molecular assessment of A. antennatus from the Western Mediterranean provides data of biological and evolutionary interest for the successful management of such a highly valuable fishery resource.

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“…However, no allozyme divergence was found (Sardà et al 1998) and the differences were explained as being a response to hydrological and ecological characteristics of the 2 Mediterranean basins (Sarà 1985). Recently, new molecular markers (microsatellites, Cannas et al 2008; mitochondrial control region, Maggio et al 2009; 16S rDNA and cytochrome c oxidase subunit I [COI], Roldán et al 2009, Sardà et al 2010 were developed for the species, but until now their application has been limited to the Western and Central Mediterranean (Maggio et al 2009, Roldán et al 2009). In both studies, no genetic structuring was found within the study area.…”

Section: Introductionmentioning

confidence: 99%

Genetic structure in the blue and red shrimp Aristeus antennatus and the role played by hydrographical and oceanographical barriers

Fernández¹,

Heras²,

Maltagliati³

et al. 2011

Mar. Ecol. Prog. Ser.

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The blue and red shrimp Aristeus antenantus supports an important commercial fishery in the Western Mediterranean, adjacent Atlantic waters and Mozambique Channel (western Indian Ocean). This study investigates its genetic structure by examining a total of 506 individuals from Mediterranean, Atlantic and Indian Ocean locations. In order to identify putative genetic stocks, sequences from 16S rDNA (546 bp) and COI (514 bp) genes were used. Genetic diversity, estimated by haplotypic and nucleotidic diversity, was lower in the Western Mediterranean than in samples from other locations. The high haplotypic diversity of the Eastern Mediterranean, Atlantic and Indian Ocean samples reflects the occurrence of a number of private haplotypes, which are also responsible for significant genetic divergence between these samples and the Western Mediterranean ones. The analysis of mismatch distributions, neutrality tests, and star-like patterns present in the network of haplotypes provided consistent inference of past population expansion in the Western Mediterranean, Atlantic and Mozambique Channel regions. Our study provides the first evidence of genetic structuring in A. antennatus across its distributional range.KEY WORDS: Aristeus antennatus · COI · 16S rDNA · Genetic structure · Atlantic Ocean · Mediterranean Sea · Western Indian Ocean Resale or republication not permitted without written consent of the publisherMar Ecol Prog Ser 421: [163][164][165][166][167][168][169][170][171] 2011 roboration of Atlantic-Mediterranean differentiation has been provided by multiple examples of the population structure of marine species, from pioneer work on crustaceans (Chthamalus montagui, Dando & Southward 1981), mollusks (Mytilus galloprovincialis, Quesada et al. 1995a,b; Sepia officinalis, Pérez-Losada et al. 1999) and fishes (Platichthys flesus, Borsa et al. 1997; Merluccius merluccius, Roldán et al. 1998) down to the present day.The blue and red shrimp Aristeus antennatus (Risso, 1816) is an important commercial deep-sea species that inhabits the Mediterranean, northeast Atlantic waters from Portugal to the Cape Verde Islands (Holthuis 1980), and the Indian Ocean from the Maldive Islands to Zanzibar, Mozambique and South Africa (Freitas 1985). In recent years, it has also been recorded off the northern coasts of Brazil (Serejo et al. 2007). A. antennatus is a eurybathic species ranging from 80 to 3300 m depth, with peaks of abundance at 600 to 900 m depth (Campillo 1994, Sardà et al. 2004). Its abundance is also geographically variable, with density in the Western Mediterranean being higher than that in the eastern basin ). Significant morphological differences have been observed in individuals from the 2 basins. However, no allozyme divergence was found (Sardà et al. 1998) and the differences were explained as being a response to hydrological and ecological characteristics of the 2 Mediterranean basins (Sarà 1985). Recently, new molecular markers (microsatellites, Cannas et al. 2008; mitochondrial control region, ...

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Isolation and characterization of 14 polymorphic microsatellite markers for the blue and red shrimp, Aristeus antennatus (Crustacea, Decapoda)

Cited by 7 publications

References 10 publications

Development and characterization of novel microsatellite markers by Next Generation Sequencing for the blue and red shrimpAristeus antennatus

Development and characterization of novel microsatellite markers by Next Generation Sequencing for the blue and red shrimpAristeus antennatus

Genetic variability of the blue and red shrimp Aristeus antennatus in the Western Mediterranean Sea inferred by DNA microsatellite loci

Genetic structure in the blue and red shrimp Aristeus antennatus and the role played by hydrographical and oceanographical barriers

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