Isolation and characterization of 12 tetra‐repeated microsatellite loci from the Formosan grass lizard (<i>Takydromus formosanus</i>)

Lin, Si Min; Wang, Chao Jun; Hsu, Yu‐Kuei; Lue, Kuang Yang; Li, Shou Hsien

doi:10.1111/j.1471-8286.2005.01136.x

Cited by 4 publications

(5 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The result supported that the Platyplacopus merged into Takydromus and negated the validity of Platyplacopus . It is also consistent with the opinions of Arnold et al 21, 22 and Lin et al 8, 23, whom supported the combination of subgenera Platyplacopus and Takydromus into Takydromus .…”

Section: Discussionsupporting

confidence: 92%

“…However, after comparing representative specimens of Platyplacopus and Takydromus , Zhao et al, argued for the validity of Platyplacopus as a full genus on the basis of its distinctness in toe structure from Takydromus 7. Based on molecular data, Lin et al and Ota et al inferred phylogenetic relationships of the genus Takydromus , and negated the validity of Platyplacopus at any taxonomic level 8, 9. Our previous analyses of 12S rRNA sequences from 14 Takydromus spp.…”

Section: Introductionmentioning

confidence: 76%

See 1 more Smart Citation

Bioinformatics Analysis the Complete Sequences of Cytochrome b of Takydromus sylvaticus and Modeling the Tertiary Structure of Encoded Protein

Chen

Tang²,

Yao³

et al. 2009

Int. J. Biol. Sci.

View full text Add to dashboard Cite

Cytochrome b (cyt b) gene complete sequences (1143bp) of Takydromus sylvaticus were sequenced. In order to clarify the phylogenetic position of the Takydromus sylvaticus, we investigated the phylogeny of 15 Takydromus spp. distributed in East-Asia by Maximum Parsimony (MP), Bayesian Inference (BI), and Maximum Likelihood (ML) methods using DNA fragments of cyt b genes. The results supported that the Platyplacopus merged into Takydromus and negated the validity of Platyplacopus. Furthermore, the prediction of tertiary structures of cyt b exhibited the CD loop region contain two short helices forming a hairpin arrangement, namely cd1 and cd2. Thermostability analysis shows that the CD-loop region is unstable thermodynamically and may provide mobility to amino acids located at the heme, and might provide high flexibility to the top of ISP (iron-sulfur protein) and the cavity region of Qo binding site. It suggested that the two short helices of CD loop region of cyt b was a dominating portion for ISP binding site.

show abstract

Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 76%

Bioinformatics Analysis the Complete Sequences of Cytochrome b of Takydromus sylvaticus and Modeling the Tertiary Structure of Encoded Protein

Chen

Tang²,

Yao³

et al. 2009

Int. J. Biol. Sci.

View full text Add to dashboard Cite

show abstract

“…Ten microsatellite loci [37] were evaluated to estimate individual heterozygosity (Table S2). The polymerase chain reactions (PCRs) were set up in a volume of 10 µl containing 50–100 ng genomic DNA, 1× PCR buffer (PROMEGA), 0.15–0.2 µM each forward and reverse primers, 2.5 mM MgCl 2 , 0.2 mM dNTP, and 0.25 U Taq DNA polymerase (PROMEGA).…”

Section: Methodsmentioning

confidence: 99%

“…The polymerase chain reactions (PCRs) were set up in a volume of 10 µl containing 50–100 ng genomic DNA, 1× PCR buffer (PROMEGA), 0.15–0.2 µM each forward and reverse primers, 2.5 mM MgCl 2 , 0.2 mM dNTP, and 0.25 U Taq DNA polymerase (PROMEGA). PCR was carried out under standard conditions, with modifications for each locus [37]. The PCR products were electrophoresed in a MegaBASE™ 1000 autosequencer (Amersham Bioscience, New Jersey, USA) with size marker ET-400 (Amersham Bioscience New Jersey, USA).…”

Section: Methodsmentioning

confidence: 99%

Sex-Specific Correlations of Individual Heterozygosity, Parasite Load, and Scalation Asymmetry in a Sexually Dichromatic Lizard

et al. 2013

Self Cite

View full text Add to dashboard Cite

Heterozygosity-fitness correlations (HFCs) provide insights into the genetic bases of individual fitness variation in natural populations. However, despite decades of study, the biological significance of HFCs is still under debate. In this study, we investigated HFCs in a large population of the sexually dimorphic lizard Takydromus viridipunctatus (Lacertidae). Because of the high prevalence of parasitism from trombiculid mites in this lizard, we expect individual fitness (i.e., survival) to decrease with increasing parasite load. Furthermore, because morphological asymmetry is likely to influence individuals' mobility (i.e., limb asymmetry) and male biting ability during copulation (i.e., head asymmetry) in this species, we also hypothesize that individual fitness should decrease with increasing morphological asymmetry. Although we did not formally test the relationship between morphological asymmetry and fitness in this lizard, we demonstrated that survival decreased with increasing parasite load using a capture-mark-recapture data set. We used a separate sample of 140 lizards to test the correlations between individual heterozygosity (i.e., standardized mean d2 and HL based on 10 microsatellite loci) and the two fitness traits (i.e., parasite load and morphological asymmetry). We also evaluated and excluded the possibility that single-locus effects produced spurious HFCs. Our results suggest male-only, negative correlations between individual heterozygosity and parasite load and between individual heterozygosity and asymmetry, suggesting sex-specific, positive HFCs. Male T. viridipunctatus with higher heterozygosity tend to have lower parasite loads (i.e., higher survival) and lower asymmetry, providing a rare example of HFC in reptiles.

show abstract

“… 2002 ; Lin et al. 2006 ). In recent years, with the development of mitochondrial DNA (mtDNA) molecular marker technology, the complete mitochondrial genome of T. wolteri and T. sexlineatus is sequenced (Yu & Ji 2013 ; Qin et al.…”

mentioning

confidence: 99%

The complete mitochondrial genome of Takydromus amurensis (Squamata: Lacertidae)

Huan

Zhao

et al. 2016

Mitochondrial DNA Part B

View full text Add to dashboard Cite

The complete mitogenome sequence of Takydromus amurensis (Squamata: Lacertidae) is determined using long PCR for the first time in this study. It is a circular molecule of 17 333 bp in length (GenBank accession number: KU641018). Similar to the most other lizards, the complete mtDNA sequence of T. amurensis contained two rRNA genes (12S rRNA and 16S rRNA), 22 tRNA genes, 13 protein-coding genes (PCGs) and a control region (D-loop). The nucleotide composition was 31.23% A, 26.06% C, 13.91% G and 28.8% T. Mitochondrial genomes analyses based on NJ method yield phylogenetic trees, including 14 reported lizards belonging to three families (Lacertidae, Gekkonidae and Agamidae). These molecular data presented here provide a useful tool for systematic analyses of genus Takydromus .

show abstract

Isolation and characterization of 12 tetra‐repeated microsatellite loci from the Formosan grass lizard (Takydromus formosanus)

Cited by 4 publications

References 13 publications

Bioinformatics Analysis the Complete Sequences of Cytochrome b of Takydromus sylvaticus and Modeling the Tertiary Structure of Encoded Protein

Bioinformatics Analysis the Complete Sequences of Cytochrome b of Takydromus sylvaticus and Modeling the Tertiary Structure of Encoded Protein

Sex-Specific Correlations of Individual Heterozygosity, Parasite Load, and Scalation Asymmetry in a Sexually Dichromatic Lizard

The complete mitochondrial genome of Takydromus amurensis (Squamata: Lacertidae)

Contact Info

Product

Resources

About