Isolation and characterization of 11 novel microsatellite DNA markers in the noble crayfish, <i>Astacus astacus</i>

Kõiv, Kuldar; Gross, Riho; Paaver, Tiit; Hurt, M.; Kuehn, Ralph

doi:10.1111/j.1365-2052.2008.01781.x

Cited by 10 publications

(3 citation statements)

References 5 publications

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“…From a subsample of 289 individuals, a total of six microsatellite loci, each with a dinucleotide repeat, were analysed using the species‐specific primer pairs Aas2, Aas6, Aas8, Aas11, Aas766 and Aas1198 (Koiv et al ., , ). The 289 individuals came from 22 populations from 12 river catchments and the hatchery in Augsburg (Table S1).…”

Section: Methodsmentioning

confidence: 99%

Phylogeography of noble crayfish (Astacus astacus) reveals multiple refugia

et al. 2013

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Summary Reconstructing the phylogeographic history of a species can aid in defining areas of conservation priority. For freshwater species, historical river structure plays a significant role in explaining genetic differentiation and population structure. However, human‐induced translocations can erase the natural genetic structure, especially for species of commercial interest such as the noble crayfish (Astacus astacus). Our aim was to reconstruct the current genetic structure of the endangered noble crayfish in central Europe to identify refugial areas that are hotspots of genetic diversity. We analysed a fragment of the mitochondrial cytochrome oxidase subunit I, and the 16S rRNA from 540 noble crayfish specimens from 156 sampling sites distributed around five European sea basins. Additionally, we conducted a microsatellite analysis of 289 individuals from 22 sites. Both mitochondrial and nuclear markers revealed genetically relatively homogenous populations in central Europe that had been influenced by anthropogenic translocations. However, some areas (Eider catchment in northern Germany and Rhineland‐Palatinate in south‐western Germany) show a distinct genetic structure with endemic haplotypes and private alleles indicating (i) that these areas were refugia for A. astacus in central Europe and (ii) that these populations have not been subject to anthropogenic translocations. Further, we found the highest genetic diversity in the Black Sea basin and particularly high differentiation between populations from the western Balkans and the remaining Black Sea populations. The split between Western Balkan and the remaining European populations is estimated to have occurred approximately 700 k years before present, whereas remaining differentiations occurred within the last 450 k years. Using migration modelling, we detected that the North Sea basin and the Baltic Sea basin were colonised independently via different colonisation paths from the eastern Black Sea basin, while the western Balkans did not contribute to this colonisation. Our results suggest the existence of at least two refugial areas in south‐eastern Europe. To conserve maximum genetic diversity, conservation priorities for noble crayfish should focus on the south‐eastern European genetic hotspots and on populations in central Europe that hold an autochthonous genetic structure (e.g. Langsee in the Eider catchment area). We further propose that each river catchment should form a separate management unit to reduce anthropogenic genetic homogenisation.

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Section: Methodsmentioning

confidence: 99%

Phylogeography of noble crayfish (Astacus astacus) reveals multiple refugia

et al. 2013

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“…SSR are highly polymorphic, abundant and evenly distributed throughout genomes (Schlötterer, 2004). This feature makes them a popular marker to analyse genetic diversity (Groeneveld et al, 2010), including of noble crayfish populations (Gross et al, 2013; Kõiv et al., 2008; Kõiv et al., 2009; Schrimpf et al, 2014; Schrimpf et al, 2017). Microsatellites provide many advantages, but they also have shortcomings.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity in natural populations of noble crayfish (Astacus astacusL.) in north-western Poland on the basis of combined SSR and AFLP data

Panicz

Napora-Rutkowski

Keszka

et al. 2019

PeerJ

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Background Conservation of noble crayfish (Astacus astacus) populations is becoming particularly important since the number of individuals is rapidly declining across the distribution range of the species in Europe. Five crayfish populations in northwestern Poland have been constantly monitored for two decades. However, the genetic structure of these populations has not been analysed, although this information is important to devise effective conservation strategies. Methods Noble crayfish were collected in the autumn of 2014 by scuba diving in Lakes Graniczne, Babinki, Biwakowe, Sęki and Kwisno, all of which are situated in the Bytów Lakeland of northwestern Poland. Genetic diversity of the five populations was assessed based on allele variability in nine SSR regions and six AFLP primer combinations. Results Microsatellite results analysed with AMOVA showed that the diversity between populations corresponds to 18% of total variability, which was confirmed by similar results obtained using AFLP. Additionally, significant genetic diversity was revealed by high average FST values. All of the studied crayfish populations significantly deviated from the expected Hardy–Weinberg genetic equilibrium and were characterised by negative values of inbreeding coefficient (FIS). Discussion The invariably negative inbreeding coefficients (FIS) suggest a low number of mating individuals, a possible consequence of the phenomenon known as genetic bottleneck. However, additional comprehensive analyses are needed to assess the genetic structure, origin and vulnerability of the remaining populations of noble crayfish in the Bytów Lakeland of northwestern Poland, which have high conservation value and are particularly important as a live genetic bank for breeding and restitution purposes.

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“…Total DNA was extracted using a protocol based on Chelex (Walsh, Metzger, & Higuchi, 1991). A set of 15 microsatellite loci (Koiv, Gross, Paaver, Hurt, & Kuehn, 2009;Koiv, Gross, Paaver, & Kuehn, 2008; Appendix S2) were analysed in one multiplex polymerase chain reaction (PCR). For the PCR, 4 μl of Type-it polymerase (Qiagen, Hilden, Germany), 4 μl of primer mix, and 0.5 μl of template, with approximately 100 ng of DNA, were used.…”

Section: Microsatellite and Statistical Analysesmentioning

confidence: 99%

Colonization history and human translocations explain the population genetic structure of the noble crayfish (Astacus astacus) in Fennoscandia: Implications for the management of a critically endangered species

Dannewitz

Palm

Edsman

2021

Aquatic Conservation

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1. The noble crayfish (Astacus astacus) is an endangered freshwater species in Europe. The main threat is from lethal crayfish plague, caused by the oomycete Aphanomyces astaci that has been spread over Europe by introduced North American crayfish species, acting as chronic carriers of the disease.2. Most of the remaining noble crayfish populations are found in the Baltic Sea area, and there is an urgent need to implement conservation actions to slow down or halt the extinction rate in this region. However, limited knowledge about the genetic structure of populations in this area has so far precluded the development of conservation strategies that take genetic aspects into consideration.3. Key objectives of this large-scale genetic study, covering 77 locations mainly from northern Europe, were to describe the contemporary population genetic structure of the noble crayfish in the Fennoscandian peninsula (Sweden, Norway, and Finland), taking postglacial colonization history into account, and to evaluate how human activities such as stocking have affected the genetic structure of the populations.4. Analyses of 15 microsatellite markers revealed three main genetic clusters corresponding to populations in northern, middle, and southern Fennoscandia, with measures of genetic diversity being markedly higher within populations in the southern cluster. The observed genetic structure probably mirrors two main colonizations of the Baltic Sea basin after the last glaciation period. At the same time, several deviations from this pattern were observed, reflecting past human translocations of noble crayfish.5. The results are discussed in relation to the conservation and management of this critically endangered species. In particular, we recommend increased efforts to protect the few remaining noble crayfish populations in southern Fennoscandia and the use of genetic information when planning stocking activities, such as reintroductions following local extinctions.

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Isolation and characterization of 11 novel microsatellite DNA markers in the noble crayfish, Astacus astacus

Cited by 10 publications

References 5 publications

Phylogeography of noble crayfish (Astacus astacus) reveals multiple refugia

Phylogeography of noble crayfish (Astacus astacus) reveals multiple refugia

Genetic diversity in natural populations of noble crayfish (Astacus astacusL.) in north-western Poland on the basis of combined SSR and AFLP data

Colonization history and human translocations explain the population genetic structure of the noble crayfish (Astacus astacus) in Fennoscandia: Implications for the management of a critically endangered species

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