Isolation and characteristics of sulfentrazone-degrading bacteria

Abstract

This study aimed to isolate and characterize bacteria able to use sulfentrazone in the commercial formulation as their sole carbon source. The isolation of the potential sulfentrazone-degrading bacteria was made from soil samples with a recent history of herbicide application and from isolates identified through rDNA sequencing. Subsequently, we assessed the growth of the isolates and their sulfentrazone degradation ability using high-performance liquid chromatography. Twenty-six potential sulfentrazone-degrading bacterial isolates were obtained in pure culture. Through analysis of the rDNA sequences, the predominance of bacterial species of the genus Pseudomonas was found. The isolates presented a differentiated ability of sulfentrazone degradation. The presence of herbicide in the culture medium reduced the log phase of four isolates. Pseudomonas putida, Pseudomonas lutea, Pseudomonas plecoglossicida and three isolates of Pseudomonas sp. showed higher sulfentrazone degradation capacity, which varied from 4 to 15%. This is the first report of the Pseudomonas genre capable of sulfentrazone degradation. The isolates obtained present potential use in bioremediation programs for soil contaminated with sulfentrazone.

Microbial activity of soil with sulfentrazone associated with phytoremediator species and inoculation with a bacterial consortium

“…The bacterial consortium used was selected based on the degradation potential presented by the isolates in a previous study (Melo et al 2016). The consortium consisted of 6 isolates identified as Pseudomonas putida, P. lutea, and P. plecoglossicida, as well as 3 isolates of Pseudomonas sp.…”

Pseudomonas spp. as growth promoting agents of sunflower and jack bean in soil with sulfentrazone

“…Half of the pots were inoculated with a bacterial consortium selected based on the potential degradation of the isolates, according to a previous study (Melo et al, 2017). The consortium consisted of six isolates identified as Pseudomonas putida, Pseudomonas lutea, Pseudomonas plecoglossicida and three isolates of Pseudomonas sp.…”

“…The selection of sulfentrazone-degrading bacteria (Melo et al, 2017), combined with their possibility of acting as plant growth promoters, can increase the chances of success and optimization of the bioremediation process. Given the above, the objective of this study was to evaluate the inoculation response of bacteria of the genus Pseudomonas in sulfentrazone-contaminated soil on the growth of jack bean (Canavalia ensiformis) and sunflower (Helianthus annuus).…”

Herbicides Tolerance in a Pseudomonas Strain Is Associated With Metabolic Plasticity of Antioxidative Enzymes Regardless of Selection

“…Even for some enzymes, there are no recent reports of the effects on bacteria, but only indirect on enzymes from bacteria-colonized plants, such as guaiacol peroxidase (GPX) (Eke et al, 2019), and ascorbate peroxidase (APX) (Fan et al, 2020). The genus Pseudomonas stands out for its tolerance to chemical stressors because of its metabolic and physiological versatility, and it can be isolated from soil, fresh water, biofilms, and other places, with potential for application in bioremediation (Venturi, 2006;Karami et al, 2016;Melo et al, 2016). Lima et al (2020) obtained collections of bacteria capable of tolerating different herbicides, including Pseudomonas sp.…”