Isolation and characterisation ofSaccharomyces cerevisiae from hydrospheres

Abstract

To isolate novel strains of Saccharomyces cerevisiae, we screened the organisms in various Japanese hydrospheres for yeast strains that are ethanol-tolerant and efficient fermenters. One such strain, which was designated TY-2, was identified as S. cerevisiae after analysing its 28S rDNA sequence and physiological properties. Unlike the reference industry strain K7, this strain assimilated cellobiose, creatine and creatinine. It also produced ethanol slightly more efficiently than K7 (92.7 ± 3.7% vs. 87.8 ± 9.4%, respectively). It did not differ from K7 in its temperature and NaCl tolerance. The cellobiose assimilation activity of TY-2 may be useful for cellulose biomass fermentation processing.

High Ethanol Production by Marine-Derived Yeasts-<i>Saccharomyces cerevisiae</i> under Stress Pressures

“…The growing cells were centrifuged at 800 g for 5 min, and the cell pellet was gathered. A portion of the pellet was cultured at 25˚C for 48 hr in 100 ml of YPD 15 liquid medium (Dglucose 150 g/l, peptone 20 g/l, and yeast extract 10 g/l).…”

“…[14] [15]. In 2015, we reported the development of a simple isolation method for S. cerevisiae with marine origins [14].…”